Regulation of Nuclear FactorKappa B on Tumor Necrosis Factorα Expression in the Liver and Its Role in Liver Injury in Rats with Acute Pancreatitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

SHI Li ¹ , TIAN Fuzhou ¹ , LI Xu ¹ , HUANG Darong ¹ , ZHAO Bi ² , GU Dayong. ²

The PLA Center of General Surgery, General Hospital of Chengdu Military Area, Chengdu 610041, China;

Corresponding author：

Keywords：

Acute pancreatitisNuclear factorkappa BTumor necrosis factorαLiver injury

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ObjectiveTo explore the regulation of nuclear factorκB (NFκB) on tumor necrosis factorα (TNFα) expression in the liver and its role in liver injury in rats with acute pancreatitis.MethodsSeventytwo Wistar rats were randomly divided into three groups: acute pancreatitis group (AP), acute pancreatitis treated with pyrrolidine dithiocarbamate (PDTC) group (APP) and sham operation group (SO). The hepatic NFκB activities were determined with electrophoretic mobility shift assays. The expressions of hepatic TNFα mRNA were detected with RTPCR. The levels of serum alanine aminotransferase (ALT) were also measured.ResultsThe NFκB activities were significantly higher in AP and APP groups than those in SO group 3-6 hours after operation. The expressions of TNFα mRNA were ber in AP and APP groups than those in SO group 3-24 hours after operation. The levels of serum ALT were also significantly higher in these two groups than those in SO group 3-24 hours after operation. However, compared with AP group, the activities of NFκB, the expressions of TNFα mRNA and the levels of ALT significantly decreased in APP group.ConclusionThe activation of hepatic NFκB is associated with the liver injury by regulating TNFα mRNA expression in acute pancreatitis.

Citation： SHI Li,TIAN Fuzhou,LI Xu,HUANG Darong,ZHAO Bi,GU Dayong.. Regulation of Nuclear FactorKappa B on Tumor Necrosis Factorα Expression in the Liver and Its Role in Liver Injury in Rats with Acute Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(3): 228-230. doi: Copy

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1. Miyahara S, Isaji S. Liver injury in acute pancreatitis and mitigation by continuous arterial infusion of an antibiotic via the superior mesenteric artery [Ｊ]. Pancreas, 2001; 23(2)∶204.
2. 石力，黄大熔，田伏洲，等. 急性胰腺炎大鼠肝脏核因子κB活化与肝损伤的关系 [Ｊ]. 世界华人消化杂志， 2002； 10（5）∶554.
3. Jaffray C, Yang J, Norman J. Elastase mimics pancreatitisinduced hepatic injury via inflammatory mediators [Ｊ]. J Surg Res, 2000; 90(2)∶95.
4. Deryckere F, Gannon F. A onehour minipreparation technique for extraction of DNAbinding proteins from animal tissues [Ｊ]. Biotechniques, 1994; 16(3)∶405.
5. Chomczynski P, Sacchi N. Singlestep method of RNA isolation by acid guanidinium thiocyanatephenolchloroform extraction [Ｊ]. Anal Biochem, 1987; 162(1)∶156.
6. Pitchumoni CS, Agarwal N, Jain NK. Systemic complications of acute pancreatitis [Ｊ]. Am J Gastroenterol, 1988; 83(6)∶597.
7. Valen G, Yan ZQ, Hansson GK. Nuclear factor kappaB and the heart [Ｊ]. J Am Coll Cardiol, 2001; 38(2)∶307.
8. Wright G, Singh IS, Hasday JD, et al. Endotoxin stressresponse in cardiomyocytes: NFkappa B activation and tumor necrosis factoralpha expression [Ｊ]. Am J Physiol Heart Circ Physiol, 2002; 282(3)∶H872.
9. Moine P, McIntyre R, Schwartz MD, et al. NFkappa B regulatory mechanisms in alveolar macrophages from patients with acute respiratory distress syndrome [Ｊ]. Shock, 2000; 13(2)∶85.
10. Udalova IA, Knight JC, Vidal V, et al. Complex NFkappa B interactions at the distal tumor necrosis factor promoter region in human monocytes [Ｊ]. J Biol Chem, 1998; 273(33)∶21178.
11. Essani NA, Fisher MA, Jaeschke H. Inhibition of NFkappa B activation by dimethyl sulfoxide correlates with suppression of TNFalpha formation, reduced ICAM1 gene transcription, and protection against endotoxininduced liver injury [Ｊ]. Shock, 1997; 7(2)∶90.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Regulation of Nuclear FactorKappa B on Tumor Necrosis Factorα Expression in the Liver and Its Role in Liver Injury in Rats with Acute Pancreatitis

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