Research on the Induction of Apoptosis in MCF7 Cells Enhanced by Thapsigargin_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

HAN Jiangtao

Department of Genaral Surgery,Yibin First People’s Hospital, Yibin 644000, Sichuan, China;

Corresponding author：

Keywords：

ThapsigarginBreast carcinomaApoptotic induction

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ObjectiveTo study the apoptotic induction effect of Thapsigargin on estrogen receptor positive human breast cancer cell lines MCF7. MethodsCells were treated with Thapsigargin and 5FU in vitro. The rate of cell apoptosis and distribution of cell cycle were detected on flow cytometry. The cell viability was measured by MTT assay and ultrastructural changes in apoptotic cells were confirmed by transmission electron microscopy.ResultsThapsigargin could increase the rates both of cell apoptosis and growth supression of MCF7 cells induced by 5FU and alter the distribution of cell cycle. Under electron microscope, apoptotic bodies in MCF7 cells considerably increased.ConclusionThapsigargin apparently enhances the effect of apoptotic induction of 5FU on MCF7 cells, it is worthy of being further studied.

Citation： HAN Jiangtao,YAO Zhenxiang. Research on the Induction of Apoptosis in MCF7 Cells Enhanced by Thapsigargin. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(3): 231-233. doi: Copy

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1. Kimura M, Lasker N, Aviv A. Thapsigarginevoked changes in human platelet Ca2+, Na+, pH and membrane potential [Ｊ]. J Physiol， 1993; 464∶ 1.
2. Korge P, Weiss JN. Thapsigargin directly induces the mitochondrial permeability transition [Ｊ]. Eur J Biochem， 1999; 265(1)∶273.
3. 周光飚，张广森. 吲哚美辛诱导K562细胞凋亡过程中半胱天冬酶及细胞内游离钙浓度的变化 [Ｊ]. 中华血液学杂志， 2001； 22（5）∶241.
4. Shen ZY, Shen WY, Chen MH, et al. Nitric oxide and calcium ions in apoptotic esophageal carcinoma cells induced by arsenite [Ｊ]. World J Gastroenterol, 2002; 8(1)∶40.
5. 杨光华主编. 病理学 [M] . 第5版. 北京：人民卫生出版社， 2001∶84～92.
6. 刘大川，姚榛祥，李非，等. 雌激素受体阴性乳癌细胞株中Cyclin D1和Ki67表达与细胞凋亡的关系 [Ｊ]. 中国普外基础与临床杂志， 2003； 10（1）∶86.
7. 姚榛祥. 乳腺癌诊治的进展 [Ｊ]. 中国普外基础与临床杂志， 2002； 9（5）∶293.
8. Lucas M, Diaz P. Thapsigargininduced calcium entry and apoptotic death of neutrophils are blocked by activation of protein kinase C [Ｊ]. Pharmacology， 2001; 63(3)∶191.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Research on the Induction of Apoptosis in MCF7 Cells Enhanced by Thapsigargin

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