ObjectiveTo study the cell apoptosis and the dynamic expression and significance of apoptosis-related genes in graft veins. MethodsA rat experimental model of autogenous graft vein was established by transplanting the right external jugular vein to infrarenal abdominal aorta in 100 Wistar rats. TUNEL and immunohistochemistry were used to detect the apoptosis, the expression of apoptosis-related genes bcl-2 and bax in vascular smooth muscle cells (VSMCs) of graft veins. ResultsWithin the 8 weeks after transplantation, the apoptotic VSMCs in the graft veins were much more than those in the control group with the apoptotic rate reaching the peak〔（28.5±16.6）%〕 on the 2nd week and dropping to （8.1±2.8）% during the 4th to 8th week. There was statistical difference compared to the control group 〔(0.5±0.2)%, P＜0.01〕. From 1 to 2 weeks, the positive rate of bcl-2 was (22.1±5.4)% which was higher than that of the control group and the 4-8 week group (P＜0.01); from 1 to 6 weeks, the expression of bax was higher than that of the control group 〔(5.5±2.3)%〕 and the postoperative 8th week group 〔(8.2±2.9)%, P＜0.01〕.Conclusionbcl-2 and bax protein may be involved in the regulation of apoptosis of VSMCs. Apoptosis of VSMCs may be an important factor in graft remodeling and graft vein stenosis.
Citation：
FENG Yong,HU Haidi,DUAN Zhiquan,LQingjie.. Dynamic Expression and Significance of Apoptosis-Related Genes bcl-2 and bax in Autogenous Graft Veins in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(4): 295-299. doi:
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| 1. |
冯勇，胡海地，段志泉，等. 自体移植静脉平滑肌细胞凋亡与增殖关系的实验研究 [Ｊ]. 中国医科大学学报, 2000； 29（2）∶103.
|
| 2. |
Reed JC. Bcl2 and the regulation of programmed cell death [Ｊ]. J Cell Biol， 1994; 124(1-2)∶1.
|
| 3. |
Oltvai ZN, Milliman CL, Korsmeyer SJ. Bcl2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death [Ｊ]. Cell， 1993; 74(4)∶609.
|
| 4. |
Gavrieli Y, Sherman Y, BenSasson SA. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation [Ｊ]. J Cell Biol， 1992; 119(3)∶493.
|
| 5. |
Bennett MR, Evan GI, Schwartz SM. Apoptosis of human vascular smooth muscle cells derived from normal vessels and coronary atherosclerotic plaques [Ｊ]. J Clin Invest， 1995; 95(5)∶2266.
|
| 6. |
冯勇，胡海地，段志泉，等.大鼠自体移植静脉术后血浆内皮素、降钙素基因相关肽与平滑肌细胞增殖的关系 [Ｊ]. 中华外科杂志， 2000； 5（38）∶400.
|
| 7. |
Krajewski S, Tanaka S, Takayama S, et al. Investigation of the subcellular distribution of the bcl2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes [Ｊ]. Cancer Res, 1993; 53(19)∶4701.
|
| 8. |
徐宏勇，李开宗，付由池，等. 雌激素受体和凋亡相关基因bcl及bax在人原发性胆囊癌中表达的临床意义 [Ｊ]. 中国普外基础与临床杂志， 2002； 9(2)∶86.
|
- 1. 冯勇，胡海地，段志泉，等. 自体移植静脉平滑肌细胞凋亡与增殖关系的实验研究 [Ｊ]. 中国医科大学学报, 2000； 29（2）∶103.
- 2. Reed JC. Bcl2 and the regulation of programmed cell death [Ｊ]. J Cell Biol， 1994; 124(1-2)∶1.
- 3. Oltvai ZN, Milliman CL, Korsmeyer SJ. Bcl2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death [Ｊ]. Cell， 1993; 74(4)∶609.
- 4. Gavrieli Y, Sherman Y, BenSasson SA. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation [Ｊ]. J Cell Biol， 1992; 119(3)∶493.
- 5. Bennett MR, Evan GI, Schwartz SM. Apoptosis of human vascular smooth muscle cells derived from normal vessels and coronary atherosclerotic plaques [Ｊ]. J Clin Invest， 1995; 95(5)∶2266.
- 6. 冯勇，胡海地，段志泉，等.大鼠自体移植静脉术后血浆内皮素、降钙素基因相关肽与平滑肌细胞增殖的关系 [Ｊ]. 中华外科杂志， 2000； 5（38）∶400.
- 7. Krajewski S, Tanaka S, Takayama S, et al. Investigation of the subcellular distribution of the bcl2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes [Ｊ]. Cancer Res, 1993; 53(19)∶4701.
- 8. 徐宏勇，李开宗，付由池，等. 雌激素受体和凋亡相关基因bcl及bax在人原发性胆囊癌中表达的临床意义 [Ｊ]. 中国普外基础与临床杂志， 2002； 9(2)∶86.
