Changes and Significance of Intercellular Adhesion Molecule-1 and Tumor Necrosis Factor-α in Intestinal Mucosa in Rats with Obstructive Jaundice_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

MIAO Huilai , LIN Musheng , CHEN Ming , CHEN Nianping , BAO Shiting.

Department of Hepatobiliary Surgery, The Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China;

Corresponding author：

Keywords：

Obstructive jaundice; Intercellular adhesion molecule-1; Tumor necrosis factor-α; Polymorphonuclear neutrophils

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Objective To investigate the changes and significance of intercellular adhesion molecule-1 (ICAM-1) and tumor necrosis factor-α (TNF-α) in intestinal mucosa in rats with obstructive jaundice. MethodsTwenty-four SD rats were randomly divided into sham operation (SO) group and bile duct ligation (BDL) group, and each group were randomly divided into the day 7 and day 14 subgroup. The expression of ICAM-1 was assayed by immunohistochemistry. The level of TNF-α was determined by ELISA. The activity of myeloperoxidase (MPO) and diamine oxidase (DAO) was determined as well.ResultsOn the day 7 and 14, in the bile duct ligation group, the ICAM-1 protein was mainly expressed in the intestinal epithelia and increased with the time on (P＜0.05); the level of TNFα increased from （14.25±1.01） ng/g to （23.83±1.43） ng/g （P＜0.01）； the intestinal DAO activity decreased from （1.70±0.36） U/mg to （1.22±0.41） U/mg （P＜0.01），and plasma DAO activity increased from （6.44±1.74）U/ml to （8.93±1.29） U/ml （P＜0.01）; the MPO activity increased from （2.85±1.22 ） U/mg to （4.93±1.37） U/mg （P＜0.01）. ConclusionThe ICAM-1 expression is significantly upregulated and the level of TNFα significantly increases after bile duct ligation, which may be involved in the PMNmediated injury to intestinal mucosa.

Citation： MIAO Huilai,LIN Musheng,CHEN Ming,CHEN Nianping,BAO Shiting.. Changes and Significance of Intercellular Adhesion Molecule-1 and Tumor Necrosis Factor-α in Intestinal Mucosa in Rats with Obstructive Jaundice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(4): 331-334. doi: Copy

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1. Wang X, Sun Z, Borjesson A, et al. Inhibition of plateletactivating factor, intercellular adhesion molecule 1 and platelet endothelial cell adhesion molecule 1 reduces experimental pancreatitisassociated gut endothelial barrier dysfunction [Ｊ]. Br J Surg， 1999; 86(3)∶ 411.
2. Jackson GD, Dai Y, Sewell WA. Bile mediates intestinal pathology in endotoxemia in rats [Ｊ]. Infect Immun， 2000; 68(8)∶4714.
3. Wardle EN, Wright NA. Endotoxin and acute renal failure associated with obstructive jaundice [Ｊ]. Br Med J， 1970; 4(733)∶472.
4. Kubes P, Arfors KE, Granger DN. Plateletactivating factorinduced mucosal dysfunction: role of oxidants and granulocytes [Ｊ]. Am J Physiol， 1991; 260(6 Pt 1)∶G965.
5. Moore EE, Moore FA, Franciose RJ, et al. The postischemic gut serves as a priming bed for circulating neutrophils that provoke multiple organ failure [Ｊ]. J Trauma, 1994; 37(6)∶881.
6. Wang X, Andersson R. The role of endothelial cells in the systemic inflammatory response syndrome and multiple system organ failure [Ｊ]. Eur J Surg, 1995; 161(10)∶703.
7. Essani NA, Fisher MA, Farhood A, et al. Cytokineinduced upregulation of hepatic intercellular adhesion molecule1 messenger RNA expression and its role in the pathophysiology of murine endotoxin shock and acute liver failure [Ｊ]. Hepatology, 1995; 21(6)∶1632.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Changes and Significance of Intercellular Adhesion Molecule-1 and Tumor Necrosis Factor-α in Intestinal Mucosa in Rats with Obstructive Jaundice

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