Objective To investigate the changes and significance of intercellular adhesion molecule-1 (ICAM-1) and tumor necrosis factor-α (TNF-α) in intestinal mucosa in rats with obstructive jaundice. MethodsTwenty-four SD rats were randomly divided into sham operation (SO) group and bile duct ligation (BDL) group, and each group were randomly divided into the day 7 and day 14 subgroup. The expression of ICAM-1 was assayed by immunohistochemistry. The level of TNF-α was determined by ELISA. The activity of myeloperoxidase (MPO) and diamine oxidase (DAO) was determined as well.ResultsOn the day 7 and 14, in the bile duct ligation group, the ICAM-1 protein was mainly expressed in the intestinal epithelia and increased with the time on (P＜0.05); the level of TNFα increased from （14.25±1.01） ng/g to （23.83±1.43） ng/g （P＜0.01）； the intestinal DAO activity decreased from （1.70±0.36） U/mg to （1.22±0.41） U/mg （P＜0.01），and plasma DAO activity increased from （6.44±1.74）U/ml to （8.93±1.29） U/ml （P＜0.01）; the MPO activity increased from （2.85±1.22 ） U/mg to （4.93±1.37） U/mg （P＜0.01）. ConclusionThe ICAM-1 expression is significantly upregulated and the level of TNFα significantly increases after bile duct ligation, which may be involved in the PMNmediated injury to intestinal mucosa.
Citation：
MIAO Huilai,LIN Musheng,CHEN Ming,CHEN Nianping,BAO Shiting.. Changes and Significance of Intercellular Adhesion Molecule-1 and Tumor Necrosis Factor-α in Intestinal Mucosa in Rats with Obstructive Jaundice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(4): 331-334. doi:
Copy
Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
| 1. |
Wang X, Sun Z, Borjesson A, et al. Inhibition of plateletactivating factor, intercellular adhesion molecule 1 and platelet endothelial cell adhesion molecule 1 reduces experimental pancreatitisassociated gut endothelial barrier dysfunction [Ｊ]. Br J Surg， 1999; 86(3)∶ 411.
|
| 2. |
Jackson GD, Dai Y, Sewell WA. Bile mediates intestinal pathology in endotoxemia in rats [Ｊ]. Infect Immun， 2000; 68(8)∶4714.
|
| 3. |
Wardle EN, Wright NA. Endotoxin and acute renal failure associated with obstructive jaundice [Ｊ]. Br Med J， 1970; 4(733)∶472.
|
| 4. |
Kubes P, Arfors KE, Granger DN. Plateletactivating factorinduced mucosal dysfunction: role of oxidants and granulocytes [Ｊ]. Am J Physiol， 1991; 260(6 Pt 1)∶G965.
|
| 5. |
Moore EE, Moore FA, Franciose RJ, et al. The postischemic gut serves as a priming bed for circulating neutrophils that provoke multiple organ failure [Ｊ]. J Trauma, 1994; 37(6)∶881.
|
| 6. |
Wang X, Andersson R. The role of endothelial cells in the systemic inflammatory response syndrome and multiple system organ failure [Ｊ]. Eur J Surg, 1995; 161(10)∶703.
|
| 7. |
Essani NA, Fisher MA, Farhood A, et al. Cytokineinduced upregulation of hepatic intercellular adhesion molecule1 messenger RNA expression and its role in the pathophysiology of murine endotoxin shock and acute liver failure [Ｊ]. Hepatology, 1995; 21(6)∶1632.
|
- 1. Wang X, Sun Z, Borjesson A, et al. Inhibition of plateletactivating factor, intercellular adhesion molecule 1 and platelet endothelial cell adhesion molecule 1 reduces experimental pancreatitisassociated gut endothelial barrier dysfunction [Ｊ]. Br J Surg， 1999; 86(3)∶ 411.
- 2. Jackson GD, Dai Y, Sewell WA. Bile mediates intestinal pathology in endotoxemia in rats [Ｊ]. Infect Immun， 2000; 68(8)∶4714.
- 3. Wardle EN, Wright NA. Endotoxin and acute renal failure associated with obstructive jaundice [Ｊ]. Br Med J， 1970; 4(733)∶472.
- 4. Kubes P, Arfors KE, Granger DN. Plateletactivating factorinduced mucosal dysfunction: role of oxidants and granulocytes [Ｊ]. Am J Physiol， 1991; 260(6 Pt 1)∶G965.
- 5. Moore EE, Moore FA, Franciose RJ, et al. The postischemic gut serves as a priming bed for circulating neutrophils that provoke multiple organ failure [Ｊ]. J Trauma, 1994; 37(6)∶881.
- 6. Wang X, Andersson R. The role of endothelial cells in the systemic inflammatory response syndrome and multiple system organ failure [Ｊ]. Eur J Surg, 1995; 161(10)∶703.
- 7. Essani NA, Fisher MA, Farhood A, et al. Cytokineinduced upregulation of hepatic intercellular adhesion molecule1 messenger RNA expression and its role in the pathophysiology of murine endotoxin shock and acute liver failure [Ｊ]. Hepatology, 1995; 21(6)∶1632.
