【Abstract】Objective To explore the changes of expression of AFP mRNA in human hepatocellular carcinoma （HCC） tissues after oral Xeloda therapy.
Methods Total RNA was extracted from HCC tissue samples collect after operation and nested reverse transcription polymerase chain reaction （RT-nested PCR） assay was performed to determine the expression of AFP mRNA in this study.
Results The final product of AFP mRNA amplified by RT-PCR was 174 bp and by RT-nested PCR was 101 bp. The AFP mRNA is positive in 12 of 21 patients (positive rate 57.14%) amplified by RT-nested PCR assay in Xeloda treatment group which is much lower than control group: 18 of 20 patients (positive rate 90.00%),P＜0.05.The serum AFP value of Xeloda treatment group 〔(23.2±12.8) μg/L〕 is much lower than that of control group 〔(39.6±24.3) μg/L〕 four weeks after operation (P＜0.05). However, There was no difference between two groups in serum AFP value before operation.
Conclusion Xeloda can effectively suppress the expression of AFP mRNA in human HCC tissues and lower it’s product serum AFP value.The clinical application of Xeloda in HCC patients deserve further study.
Citation：
LI Li,WU Chunbo,SUN Feng,RAN Jianghua,LI Xiaoyan.. Experimental Study on Changes of Expression of AFP mRNA in Human Hepatocellular Carcinoma Tissues after Oral Xeloda Therapy. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(5): 401-404. doi:
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- 1. Komeda T, Fukuda Y, Sando T, et al. Sensitive detection of circulating hepatocellular carcinoma cells in peripheral venous blood [Ｊ]. Cancer, 1995; 75(9)∶2214.
- 2. Kedda MA, Kew MC, Skelton M, et al. Nonspecificity of messenger RNA of alphafetoprotein in peripheral blood in detecting early spread of hepatocellular carcinoma in black Africans [Ｊ]. J Gastroenterol Hepatol, 1998; 13(9)∶885.
- 3. 刘迎娣，梁敏，孙晓华，等.肝癌组织甲胎蛋白mRNA的定量检测与临床意义 [Ｊ]. 解放军医学杂志，2000； 25(4)∶279.
- 4. 杨大明，姚登福，朱云松，等.人肝癌组织和外周血甲胎蛋白基因片段扩增及临床应用 [Ｊ]. 中国实验诊断学, 2003； 9(1)∶38.
- 5. Jiang SY, Shyu RY, Huang MF, et el. Detection of alphafetoproteinexpressing cells in the blood of patients with hepatoma and hepatitis [Ｊ]. Br J Cancer, 1997; 75(6)∶928.
- 6. 胡成进，杨道理，李劲松.人肝细胞癌中AFP基因的表达 [Ｊ].中国肿瘤临床，1998； 25（5）∶341.
- 7. Pickard M, Dive C, Kinsella AR. Differences in resistance to 5fluorouracil as a function of cell cycle delay and not apoptosis [Ｊ]. Br J Cancer, 1995; 72(6)∶ 1389.
- 8. Olivier M, Eric S,Arlette H, et al. Sensitization of cancer cells treated with cytotoxic drugs to Fasmediated cytotoxicity [Ｊ]. Journal of the National Cancer Institute, 1997; 89(11)∶784.
- 9. 周志宏，郑建伟，商少宏，等.TNF和5Fu联合诱导结肠癌细胞凋亡及bcl2基因表达的实验研究 [Ｊ]. 肿瘤研究与临床， 2001； 13(16)∶ 377.
- 10. 杨连粤，易彤波. Caspas8、Caspase3与Fas介导凋亡信号传导及其在肝癌细胞凋亡中的作用 [Ｊ].中国现代医学杂志，2000； 10(8)∶27.
