Specific Expression and Distribution of Toll-Like Receptor 4 in Rat Pancreatic Tissue_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Yuan ¹ , ZHOU Zongguang ¹ , ZHANG Jie ² , ZHENG Xuelian ¹ , HU Tingze. ³

Gastroenterological Surgery &;
Institute of Digestive Surgery, West China Hospital, Sichuan University, Chengdu 610041,China;

Corresponding author：

Keywords：

Pancreas Innate immunity Toll-like receptor 4

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【Abstract】Objective To explore Toll-like receptor 4 (TLR4) expression and distribution in rat pancreas.
Methods Reverse transcriptase-polymerase chain reaction (RTPCR) and immunohistochemistry (IHC) were applied to detect expression of TLR4-mRNA and TLR4 protein respectively.
Results RT-PCR of RNA isolated from rat pancreatic tissue yielded the predicted amplicon for the TLR4. IHC/immunofluorescence revealed TLR4 protein mainly distributed in the epithelium of the pancreatic duct, vascular endothelium of the exocrine section, endocrine islet also had some signs of distribution. No TLR4 protein signal could be detected in the acinar cells.
Conclusion TLR4 could be detected in rat pancreas. Its distribution is consistent with its roles in immune surveillance, mainly in tissues exposed to the external environment such as pancreatic duct as well as in immunologically important settings such as pancreatic vascular endothelium. Islet also has some signs of distribution. No TLR4 expression in acinar cells, suggesting TLR4 immunological involvement in the pathophysiology of pancreas.

Citation： LI Yuan,ZHOU Zongguang,ZHANG Jie,ZHENG Xuelian,HU Tingze.. Specific Expression and Distribution of Toll-Like Receptor 4 in Rat Pancreatic Tissue. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(5): 420-423. doi: Copy

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1. Modlin RL, Brightbill HD, Godowski PJ. The toll of innate immunity on microbial pathogens [Ｊ]. N Engl J Med， 1999; 340(23)∶1834.
2. Johnson GB, Brunn GJ, Platt JL. Cutting edge: an endogenous pathway to systemic inflammatory response syndrome (SIRS)like reactions through Tolllike receptor 4 [Ｊ]. J Immunol， 2004; 172(1)∶20.
3. Kobayashi K, Hernandez LD, Galan JE, et al. IRAKM is a negative regulator of Tolllike receptor signaling [Ｊ]. Cell, 2002; 110(2)∶191.
4. Mak TW, Yeh WC. Immunology: a block at the toll gate [Ｊ]. Nature, 2002; 418(6900)∶835.
5. Lien E, Ingalls RR. Tolllike receptors [Ｊ]. Crit Care Med, 2002; 30(1 Suppl)∶S1.
6. Akira S. Mammalian Tolllike receptors [Ｊ]. Curr Opin Immunol, 2003; 15(1)∶5.
7. Konok GP, Thompson AG. Pancreatic ductal mucosa as a protective barrier in the pathogenesis of pancreatitis [Ｊ]. Am J Surg， 1969; 117(1)∶18.
8. Arendt T. Bileinduced acute pancreatitis in cats. Roles of bile, bacteria, and pancreatic duct pressure [Ｊ]. Dig Dis Sci， 1993; 38(1)∶39.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Specific Expression and Distribution of Toll-Like Receptor 4 in Rat Pancreatic Tissue

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