JIN Bin 1 , JIANG Xihong 2 , WANG Xuehao 1 , ZHANG Feng 1 , WANG Wei 3 , LIU Xianxi. 3
  • Department of Liver Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;
Gallbladder carcinoma Telomerase Ribozyme Gene expression
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【Abstract】Objective  To design the hammerhead ribozyme gene according to the hTR sequence in the gallbladder cancer cell, and build it into the eukaryon expression vector pTriEx-4.
Methods  According to the hTR cDNA sequence, the authors designed the primers and take the hTR template area gene from the gallbladder cancer cells by RT-PCR.The hammerhead ribozyme gene was synthesize according to the result of sequencing, and combine them with eukaryon expressing vector. Identified the exactitude of recombine vector by digestion.
Results  The 68 bp sequence extracted from the cell through the RT-PCR had the same template sequence comparing with the hTR cDNA. The recombinant plasmid with the hammerhead ribozyme gene was correct by digestion identification.
Conclusion  The RT-PCR method can extract the gallbladder cancer cell’s hTR gene. We construct the eukaryon expression vector containing the hammerhead ribozyme gene successfully which is the foundation for gene therapy of gallbladder cancer.

Citation： JIN Bin,JIANG Xihong,WANG Xuehao,ZHANG Feng,WANG Wei,LIU Xianxi.. Constructing the Hammerhead Ribozyme Gene’s Eukaryon Express Vectors for hTR Sequence of Gallbladder Carcin-oma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(5): 436-439. doi: Copy