Experimental Study on Allotransplantation of Cultured Parathyroid Cells in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

DUAN Xiuqing ¹ , SONG Chun ² , WANG Jiangbin ¹ , SHI Yubo ² , XU Ping ¹ , SONG Chunfang. ¹

Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China;

Corresponding author：

Keywords：

Parathyroid gland Cell culture Transplantation

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【Abstract】Objective To study the influence of transplantation of cultured parathyroid cells on the survival of the allografts in rats.
Methods Parathyroid cells digested with collagenase and trypsin were cultured and transplanted under the left renal capsule. The survival time of the allografts was recorded and the allografts were examined by transmission electron microscopy.
Results In fresh parathyroid cells group, the mean survival time was (9.25±3.45) days. While in cultured parathyroid cells group, the survival time was (46.25±7.44) days (P＜0.01). During the 50 days of observation, serum calcium and PTH remained normal in 6 of 8 rats. There were intact parathyroid cells in the allografts which had abundant rough endoplasmic reticula,mitochondria and secretory granules.
Conclusion Transplantation of cultured parathyroid cells in rats can prolong the survival time of allografts and is a potent way to cure hypoparathyroidism.

Citation： DUAN Xiuqing,SONG Chun,WANG Jiangbin,SHI Yubo,XU Ping,SONG Chunfang.. Experimental Study on Allotransplantation of Cultured Parathyroid Cells in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(6): 486-488. doi: Copy

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1. Tolloczko T, Wozniewicz B, Sawicki A, et al. Allotransplantation of cultured human parathyroid cells: present status and perspectives [Ｊ]. Transplant Proc, 1997; 29(1-2)∶998.
2. Starling JR, Fidler R, Corry RJ. Prolongation of survival of rat parathyroid allografts by enhancing serum and tissue culture [Ｊ].Surgery, 1977; 81(6)∶668.
3. Feind CR, Weber CJ, Derenoncourt F, et al. Survival and allotransplantation of cultured human parathyroids [Ｊ].Transplant Proc, 1979; 11(1)∶1011.
4. Schachter P, Christy MD, Leight GS Jr, et al. Function in athymic nude mice of parathyroid heterografts from patients with primary hyperparathyroidism and secondary hyperparathyroidism [Ｊ].Surgery, 1990; 108(6)∶1040.
5. Tsuji K, Fuchinoue S, Kai K, et al. Culture of human parathyroid cells for transplantation [Ｊ].Transplant Proc, 1999; 31(7)∶2697.
6. Faustman D, Coe C. Prevention of xenograft rejection by masking donor HLA class I antigens [Ｊ]. Science, 1991; 252(5013)∶1700.
7. 王颜刚, 王桂兰. 甲状旁腺移植的进展 [Ｊ]. 国外医学外科学分册, 1994; 21(3)∶143.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Experimental Study on Allotransplantation of Cultured Parathyroid Cells in Rats

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