Studies on Induction of Experimental Autoimmune Thyroiditis with Excessive Iodine and Thyroglobulin in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

GAO Fuzhi ¹ , GENG Zhihui ² , CHEN Xiaomei ³ , YAN Jingze ¹ , ZHANG Deheng ⁴ , ZHENG Zelin. ⁴

Department of General Surgery, The 461Hospital of PLA, Changchun 130021, China;

Corresponding author：

Keywords：

Excessive iodine Thyroglobulin Autoimmune thyroiditis Rat

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【Abstract】Objective To study the relation between iodine and experimental autoimmune thyroiditis(EAT).
Methods Establishment of animal model was performed with iodine and thyroglobulin(TG).The rats were randomly divided into 5 groups: normal control group(NC), low iodine group (LI,500 μg/L), high iodine group(HI,500 mg/L), TG+Freund adjuvant group(TG) and TG+Freund adjuvant+HI group(TG+HI). The rats in TG group and TG+HI group were rejected hypodermically with TG emulsified by complete Freund adjuvant, and strengthen immunity was conducted with TG emulsified by incomplete Freund adjuvant on 15 days. After that, strengthen immunity was done weekly till the end of the experiment. Serum TGAb and TPOAb were measured by radioimmunoassay. Observation of the pathological changes of thyroid gland was also done.
Results Thyroid follicular destruction and lymphocytic infiltration in the TG+HI group (3.83±1.72) and HI group (3.00±0.89) were significantly higher than that of the NC group(0.67±0.82)，P＜0.05. The results of the TG group were higher compared with the NC group, but there were no significant differences between them（P gt;0.05）. The levels of TGAb in the TG+HI (4.990±1.505)，HI (3.589±1.240) and TG group (4.883±1.198) were significant higher than those of the NC group (0.642±0.454) and the LI group (0.707±0.240)，P＜0.01. The levels of TPOAb in TG+HI group (1.475±0.523) and TG group (1.316±0.606) were significantly higher than those of the NC group (0.365±0.196) and the LI group（P＜0.01）. Serum TGAb and TPOAb levels were positively correlated with the histological grades of lymphocytic thyroiditis(r=0.9,P＜0.05）.
Conclusion Excessive iodine intake may induce the occurrence of EAT. The induction of EAT with excessive iodine and TG may be more efficient.

Citation： GAO Fuzhi,GENG Zhihui,CHEN Xiaomei,YAN Jingze,ZHANG Deheng,ZHENG Zelin.. Studies on Induction of Experimental Autoimmune Thyroiditis with Excessive Iodine and Thyroglobulin in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(6): 489-492. doi: Copy

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2.	Allen EM, Appel MC, Braverman LE. The effect of iodide ingestion on the development of spontaneous lymphocytic thyroiditis in the diabetesprone BB/W rat [Ｊ]. Endocrinology， 1986; 118(5)∶1977.
3.	Tomer Y. Antithyroglobulin autoantibodies in autoimmune thyroid diseases: crossreactive or pathogenic? [Ｊ]. Clin Immunol Immunopathol， 1997; 82(1)∶3.
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9.	田恩江,张佳丽,赵树君,等. 碘对诱发易感和非易感小鼠自体免疫甲状腺炎的实验研究 [Ｊ]. 中国地方病学杂志, 2003; 1(1)∶5.
10.	Rodien P, Madec AM, Ruf J, et al. Antibodydependent cellmediated cytotoxicity in autoimmune thyroid disease: relationship to antithyroperoxidase antibodies [Ｊ]. J Clin Endocrinol Metab, 1996; 81(7)∶2595.
11.	Harach HR, Escalante DA, Onativia A, et al. Thyroid carcinoma and thyroiditis in an endemic goitre region before and after iodine prophylaxis [Ｊ]. Acta Endocrinol (Copenh), 1985; 108(1)∶55.
12.	房维堂，王金彪，李平春，等. 引高碘水居民甲状腺功能和甲状腺的自身免疫状况 [Ｊ]. 中国地方病杂志, 1994; 3(1)∶9.
13.	Park YK, Harland BF, Vanderveen JE, et al. Estimation of dietary iodine intake of Americans in recent years [Ｊ]. J Am Diet Assoc, 1981; 79(1)∶17.
14.	Safran M, Paul TL, Roti E, et al. Environmental factors affecting autoimmune thyroid disease [Ｊ]. Endocrinol Metab Clin North Am, 1987; 16(2)∶327.
15.	Ahmann AJ, Burman KD. The role of T lymphocytes in autoimmune thyroid disease [Ｊ]. Endocrinol Metab Clin North Am, 1987; 16(2)∶287.
16.	龙振洲. 医学免疫学 [M]. 北京: 人民卫生出版社，1995∶53~63.
17.	Many MC, Papadopoulos J, Martin C, et al. Iodine induced damage in mouse hyperlastic thyroid is associated to lipid peroxidation. In: Gordon Gross & Hennemann. eds. Progress in Thyroid Research [M]. Rotterdam： Balkema 1991∶635-637.
18.	Bagchi N, Brown TR, Urdanivia E, et al. Induction of autoimmune thyroiditis in chickens by dietary iodine [J]. Science, 1985; 230(4723)∶325.

1. Li M, Eastman CJ, Boyages SC. Iodine induced lymphocytic thyroiditis in the BB/W rat: early and late immune phenomena [Ｊ]. Autoimmunity， 1993; 14(3)∶181.
2. Allen EM, Appel MC, Braverman LE. The effect of iodide ingestion on the development of spontaneous lymphocytic thyroiditis in the diabetesprone BB/W rat [Ｊ]. Endocrinology， 1986; 118(5)∶1977.
3. Tomer Y. Antithyroglobulin autoantibodies in autoimmune thyroid diseases: crossreactive or pathogenic? [Ｊ]. Clin Immunol Immunopathol， 1997; 82(1)∶3.
4. 朱惠民,王风荣,尹桂山,等.高碘地方性甲状腺肿发病机理的实验研究 [Ｊ]. 中国地方病学杂志, 1989； 8(4)∶220.
5. Rasooly L, Burek CL, Rose NR. Iodineinduced autoimmune thyroiditis in NODH2h4 mice [Ｊ]. Clin Immunol Immunopathol， 1996; 81(3)∶287.
6. 孙崴,宋光华,贺斌. 碘和甲状腺球蛋白诱导大鼠实验性自身免疫性甲状腺炎的研究 [Ｊ]. 中华内科杂志, 2000; 39(12)∶841.
7. 戴蔼善，马冰彬，陈恒，等. 自体免疫甲状腺炎在上海地区的人群患病情况及其诊断标准探讨 [Ｊ]. 中华内分泌代谢杂志， 1989; 5（2）∶67.
8. 王聆，郑五飞. ELISA测定TMAb和TGAb的临床意义 [Ｊ]. 中华内分泌代谢杂志， 1989； 5（2）∶70.
9. 田恩江,张佳丽,赵树君,等. 碘对诱发易感和非易感小鼠自体免疫甲状腺炎的实验研究 [Ｊ]. 中国地方病学杂志, 2003; 1(1)∶5.
10. Rodien P, Madec AM, Ruf J, et al. Antibodydependent cellmediated cytotoxicity in autoimmune thyroid disease: relationship to antithyroperoxidase antibodies [Ｊ]. J Clin Endocrinol Metab, 1996; 81(7)∶2595.
11. Harach HR, Escalante DA, Onativia A, et al. Thyroid carcinoma and thyroiditis in an endemic goitre region before and after iodine prophylaxis [Ｊ]. Acta Endocrinol (Copenh), 1985; 108(1)∶55.
12. 房维堂，王金彪，李平春，等. 引高碘水居民甲状腺功能和甲状腺的自身免疫状况 [Ｊ]. 中国地方病杂志, 1994; 3(1)∶9.
13. Park YK, Harland BF, Vanderveen JE, et al. Estimation of dietary iodine intake of Americans in recent years [Ｊ]. J Am Diet Assoc, 1981; 79(1)∶17.
14. Safran M, Paul TL, Roti E, et al. Environmental factors affecting autoimmune thyroid disease [Ｊ]. Endocrinol Metab Clin North Am, 1987; 16(2)∶327.
15. Ahmann AJ, Burman KD. The role of T lymphocytes in autoimmune thyroid disease [Ｊ]. Endocrinol Metab Clin North Am, 1987; 16(2)∶287.
16. 龙振洲. 医学免疫学 [M]. 北京: 人民卫生出版社，1995∶53~63.
17. Many MC, Papadopoulos J, Martin C, et al. Iodine induced damage in mouse hyperlastic thyroid is associated to lipid peroxidation. In: Gordon Gross & Hennemann. eds. Progress in Thyroid Research [M]. Rotterdam： Balkema 1991∶635-637.
18. Bagchi N, Brown TR, Urdanivia E, et al. Induction of autoimmune thyroiditis in chickens by dietary iodine [J]. Science, 1985; 230(4723)∶325.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Studies on Induction of Experimental Autoimmune Thyroiditis with Excessive Iodine and Thyroglobulin in Rats

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