【Abstract】Objective To explore the dynamic expression of TNF-α and VEGF in the development of esophageal varices in rats with portal hypertension.
Methods Sixty male SD rats were randomly divided into the experimental group and the control group. In the experimental group, a two-stage ligation of portal vein plus ligation of the left adrenal vein was performed.After establishment of the model, the expression of TNF-α、VEGF and PCNA in the lower esophagus was detected with immunohistochemical SP technique on 7 d、14 d、21 d and comparision of these data with control group was performed respectively. In the control group, a sham-operation was performed, was also divided.
Results The portal venous pressure in the experimental group was significantly higher than that of the control, so did the vessel number and the total vascular area of the submucosal veins in the lower esophagus. Compared with the control subgroups, the expression of TNF-α and VEGF on the 21 d subgroup was ber, while PCNA was ber on the 14 d and 21 d.
Conclusion In the development of esophageal varices of portal hypertension, VEGF possibly plays a role in the varices developemt, and TNF-α may be responsible for the damage of esophageal mucosa.
Citation：
YIN Zhaohui,LIU Xunyang,LU Huanyuan,HUANG Ranglang,REN Shuping.. Expression and Significance of TNF-α and VEGF in Development of Esophageal Varices in Portal Hypertensive Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(6): 508-511. doi:
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Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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- 1. Tanoue K, Kitano S, Hashizume M, et al. A rat model of esophageal varices [Ｊ]. Hepatology, 1991; 13(2)∶ 353.
- 2. LopezTalavera JC, Merrill WW, Groszmann RJ. Tumor necrosis factor alpha: a major contributor to the hyperdynamic circulation in prehepatic portalhypertensive rats [Ｊ]. Gastroenterology, 1995; 108(3)∶761.
- 3. LopezTalavera JC, Cadelina G, Olchowski J, et al. Thalidomide inhibits tumor necrosis factor alpha, decreases nitric oxide synthesis, and ameliorates the hyperdynamic circulatory syndrome in portalhypertensive rats [Ｊ]. Hepatology, 1996; 23(6)∶1616.
- 4. Ohta M, Tarnawski AS, Itani R, et al. Tumor necrosis factor alpha regulates nitric oxide synthase expression in portal hypertensive gastric mucosa of rats [Ｊ]. Hepatology, 1998; 27(4)∶906.
- 5. Marsden PA, Brenner BM. Transcriptional regulation of the endothelin1 gene by TNFalpha [Ｊ]. Am J Physiol, 1992; 262(4 Pt 1)∶C854.
- 6. Shalaby F, Rossant J, Yamaguchi TP, et al. Failure of bloodisland formation and vasculogenesis in Flk1deficient mice [Ｊ]. Nature, 1995; 376(6535)∶62.
- 7. Fong GH, Rossant J, Gertsenstein M, et al. Role of the Flt1 receptor tyrosine kinase in regulating the assembly of vascular endothelium [Ｊ]. Nature, 1995; 376(6535)∶66.
- 8. Kroll J, Waltenberger J. VEGFA induces expression of eNOS and iNOS in endothelial cells via VEGF receptor2 (KDR) [Ｊ]. Biochem Biophys Res Commun, 1998; 252(3)∶743.
- 9. Kroll J, Waltenberger J. A novel function of VEGF receptor2 (KDR): rapid release of nitric oxide in response to VEGFA stimulation in endothelial cells [Ｊ]. Biochem Biophys Res Commun, 1999; 265(3)∶636.
- 10. Celis JE, Madsen P, Nielsen S, et al. Nuclear patterns of cyclin (PCNA) antigen distribution subdivide Sphase in cultured cellssome applications of PCNA antibodies [Ｊ].Leuk Res, 1986; 10(3)∶237.
