Objective To study the relationship between the expression of p53，bcl-2 and nm23 and clinicopathology in the patients with gastric cancer and to probe the correlation among the expression of three kinds of oncogene.Methods Eighty cases of different differentiated gastric cancer, 37 cases of peri-tumor atypical mucosa hyperplasia and 20 cases of normal gastric mucosa were stained by immunohistochemistry (SP method).The expression of p53,bcl-2 and nm23 was analyzed with their relation to histologic type，differentiation，lymph node metastasis and prognosis of gastric carcinoma.Results The positive rates of bcl-2 protein expression in mild,middle and severe peritumor atypical hyperplasia were 7.1%, 18.1% and 25.0%. There was no obvious difference among 3 groups (P gt;0.05). The bcl-2 positive rates of welldifferentiated and poordifferentiated gastric cancer were 78.2% and 48.5% respectively, the difference was obvious (P＜0.05). The positive expression rate of p53 protein in welldifferentiated gastric cancer was 72.5%, obviously lower than that of poordifferentiated gastric cancer (86.2%,P＜0.05). The positive rate of nm23 in welldifferentiated gastric cancer was 84.3%, obviously higher than that of poordifferentiated (17.2%),and the expression rate of nonlymph node metastasis group was higher than that of lymph node metastasis group (P＜0.05). The expression rate of nm23 protein was closely correlated to tumor differentiation and lymph node metastasis (P＜0.05). There was a negative correlation between p53 and bcl-2 expression. Conclusion bcl-2 and p53 can be important indices for tumor differentiation and prognosis. nm23 protein plays an important inhibiting role in the process of gastric cancer metastasis and may be a molecular biological basis for the evaluation of patients prognosis in gastric cancer.
Citation：
ZHU Xinyong,ZHANG Tongquan,WANG Fuchun,FANG Chihua,MA Junxun,ZHANG Wei,CAO Rui. Relation of p53，bc1-2 and nm23 Protein Expression to the Biological Characteristics of Gastric Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(6): 589-592. doi:
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- 1. 詹镕洲. p53、 cerb B2基因在乳腺癌预后中的意义 [Ｊ]. 中国普外基础与临床杂志， 1998； 5（5）∶298.
- 2. 骆成玉， 李世拥. 大肠癌bcl2基因表达和重排的研究 [Ｊ].中国普外基础与临床杂志， 1999； 6（1）∶4.
- 3. Silvestrini R, Veneroni S， Daidone MG, et al. The Bcl2 protein: a prognostic indicator strongly related to p53 protein in lymph nodenegative breast cancer patients [Ｊ]. J Natl Cancer Inst, 1994； 86(7)∶499.
- 4. Steeg PS， Bevilacque G, Kopper L, et al. Evidence for a novel gene associated with low tumor metastatic potential [Ｊ]. J Natl Cancer Inst, 1988; 80（3）∶200.
- 5. 谷化平, 倪灿荣, 尚培中, 等. 乳腺癌组织中CD44V6和nm23H1的mRNA表达及意义 [Ｊ].中国普外基础与临床杂志, 2001; 8（5）∶307.
- 6. 谭建美， 方国恩， 闻兆章， 等. 胃癌中nm23蛋白表达及临床意义 [Ｊ]. 中国普外基础与临床杂志, 1999; 6（3）∶129.
- 7. Kodera Y, Isobe K, Yamauchi M, et al. Expression of nm23H1 RNA levels in human gastric cancer tissue. A negative correlation with nodal metastasis [Ｊ].Cancer, 1994; 73(2)∶259.
- 8. Ura H, Denno R, Hirata K. The significance of nm23 protein expression in human gastric carcinoma [Ｊ]. Surg Today, 1996; 26(12)∶957.
- 9. Yoshida K, Yasui W, Yokozaki H, et al. New molecular prognostic markers in gastric carcinoma [Ｊ].Gan To Kagaku Ryoho, 1998; 25(13)∶2021.
- 10. 张声， 叶圣华， 林华. p53和p21过表达与胃癌淋巴结转移呈正相关 [Ｊ].中国肿瘤临床， 1996; 23（9）∶639.
- 11. Mosnier JF, Perret AG, Vindimian M, et al. An immunohistochemical study of the simultaneous expression of bcl2 and p53 oncoproteins in epithelial tumor of the colon and rectum [Ｊ]. Arch Pathal Lab Med, 1996; 120(7)∶654.
