ObjectiveTo study the relationship between hepatocellular apoptosis and glycogen contents during hepatic cold preservationreperfusion and its mechanism.MethodsBased on the model of four groups of rabbit livers with different hepatocellular glycogen contents, hepatocellular apoptosis and bax gene expression were observed during hepatic cold preservationreperfusion.ResultsApoptotic hepatocytes were obviously found in 60 minute reperfusing livers subsequent to 9 hour cold storage, and there was significant difference in the numbers of apoptotic hepatocytes among all the groups. In the same time, there was the close relationship between the levels of bax gene expression and the glycogen contents of hepatocytes.ConclusionIntracellular abundant glycogen may significantly depress the hepatocellular apoptosis during hepatic cold preservationreperfusion by decreasing hepatocellular bax gene expression.
Citation：
TANG Lijun,TIAN Fuzhou,WANG Yu,GAO Xiaomei. Relationship Between Hepatocellular Apoptosis and Glycogen Contents During Hepatic Cold PreservationReperfusion and Its Mechanism. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(3): 226-229. doi:
Copy
Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
| 1. |
Thiel M,Imendorffer S,Chouker A,et al. Expression of adhesion molecules on circulating polymorphonuclear leukocytes during orthotopic liver transplantation [Ｊ]. Hepatology， 1998; 28(6)∶1538.
|
| 2. |
Jamieson NV,Lindell S, Sundberg R, et al.An analysis of the components in UW solution using the isolated perfused rabbit liver [Ｊ]. Transplantation, 1988; 46(4)∶512.
|
| 3. |
汤礼军,田伏洲,王雨, 等. 兔肝脏保存再灌注损伤模型的建立及评价 [Ｊ]. 肝胆外科杂志， 1998; 6（2）∶123.
|
| 4. |
Cywes R, Greig PD, Sanabria JR, et al. Effect of intraportal glucose infusion on hepatic glycogen content and degradation, and outcome of liver transplantation [Ｊ]. Ann Surg， 1992； 216（3）∶235.
|
| 5. |
Gavrieli Y, Sherman Y, BenSasson SA. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation [Ｊ]. J Cell Biol， 1992; 119（3）∶493.
|
| 6. |
王雨, 田伏洲, 汤礼军, 等. 兔移植肝糖原在缺血再灌注期间的代谢特点 [Ｊ]. 中华普通外科杂志， 2001; 16(7)∶442.
|
| 7. |
Schumer M, Colombel MC, Sawczuk IS, et al. Morphologic，biochemicals， and molecular evidence of apoptosis during the reperfusion phase after brief periods renal ischemia [Ｊ]. Am J Pathol, 1992; 140(4)∶831.
|
| 8. |
汤礼军, 田伏洲, 王雨, 等. 离体肝脏低温保存再灌注期间肝细胞凋亡现象的观察 [Ｊ]. 中国普外基础与临床杂志， 2000; 7(4)∶214.
|
| 9. |
Jurgensmeier JM, Xie Z, Deveraux Q, et al. Bax directly induces release of cytochrome C from isolated mitochondria [Ｊ]. Proc Natl Acad Sci USA， 1998; 95(9)∶4997.
|
- 1. Thiel M,Imendorffer S,Chouker A,et al. Expression of adhesion molecules on circulating polymorphonuclear leukocytes during orthotopic liver transplantation [Ｊ]. Hepatology， 1998; 28(6)∶1538.
- 2. Jamieson NV,Lindell S, Sundberg R, et al.An analysis of the components in UW solution using the isolated perfused rabbit liver [Ｊ]. Transplantation, 1988; 46(4)∶512.
- 3. 汤礼军,田伏洲,王雨, 等. 兔肝脏保存再灌注损伤模型的建立及评价 [Ｊ]. 肝胆外科杂志， 1998; 6（2）∶123.
- 4. Cywes R, Greig PD, Sanabria JR, et al. Effect of intraportal glucose infusion on hepatic glycogen content and degradation, and outcome of liver transplantation [Ｊ]. Ann Surg， 1992； 216（3）∶235.
- 5. Gavrieli Y, Sherman Y, BenSasson SA. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation [Ｊ]. J Cell Biol， 1992; 119（3）∶493.
- 6. 王雨, 田伏洲, 汤礼军, 等. 兔移植肝糖原在缺血再灌注期间的代谢特点 [Ｊ]. 中华普通外科杂志， 2001; 16(7)∶442.
- 7. Schumer M, Colombel MC, Sawczuk IS, et al. Morphologic，biochemicals， and molecular evidence of apoptosis during the reperfusion phase after brief periods renal ischemia [Ｊ]. Am J Pathol, 1992; 140(4)∶831.
- 8. 汤礼军, 田伏洲, 王雨, 等. 离体肝脏低温保存再灌注期间肝细胞凋亡现象的观察 [Ｊ]. 中国普外基础与临床杂志， 2000; 7(4)∶214.
- 9. Jurgensmeier JM, Xie Z, Deveraux Q, et al. Bax directly induces release of cytochrome C from isolated mitochondria [Ｊ]. Proc Natl Acad Sci USA， 1998; 95(9)∶4997.
