ObjectiveTo investigate the expression of proapoptosis gene bad in intrahepatic cholangiocarcinoma (ICC) and its relationship to differentiation of the tumor.MethodsThe immunohistochemistry technique by Dako Envision system and rabbit antihuman bad polyclonal antibodies were adopted. The expression of bad was detected in 48 cases of ICC and 25 cases of control tissues.ResultsBad immunoreactivity in 48 cases of ICC was higher than that of bile duct epithelium in 25 cases of control tissues. And contrasted with 21 cases of well differentiated ICC, bad immunoreactivity was higher in 27 cases of middle and poor differentiated ICC.ConclusionThe expression of bad gene may be related to the differentiation of ICC.
Citation：
YAN Qingguo,SHI Jianguo,WANG Wenyong,LI Yusong,HUANG Gaosheng,WANG Wenliang. The Expression of ProApoptosis Gene Bad in Intrahepatic Cholangiocarcinoma and Its Relationship to Differentiation of the Tumor. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(3): 253-255. doi:
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Hattori T, Ookawa N, Fujita R, et al. Heterodimerization of bcl2 and bclx(L) with bax and bad in colorectal cancer [J]. Acta Oncol, 2000; 39(4)∶495.
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- 1. Van Cruchten S, Van Den Broeck W. Morphological and biochemical aspects of apoptosis, oncosis and necrosis [J]. Anat Histol Embryol, 2002; 31(4)∶214.
- 2. 闫庆国，王文亮，黄高昇. 促细胞凋亡bax在胆管癌中的表达与意义 [J]. 第四军医大学学报，1997； 18（2）∶139.
- 3. 闫庆国，王文亮，黄高昇. 胆管癌中抗凋亡基因bcl2的表达及意义 [J]. 临床肝胆病杂志，1997； 13（4）∶194.
- 4. Ottilie S, Diaz JL, Horne W, et al. Dimerization properties of human BAD. Identification of a BH3 domain and analysis of its binding to mutant bcl2 and bclxL proteins [J]. J Biol Chem,1997; 272(49)∶30866.
- 5. Kelekar A, Chang BS, Harlan JE, et al. Bad is a BH3 domaincontaining protein that forms an inactivating dimer with bclxL [J]. Mol Cell Biol, 1997; 17(12)∶7040.
- 6. Hong JR, Wu JL.Induction of apoptotic death in cells via bad gene expression by infectious pancreatic necrosis virus infection [J]. Cell Death Differ, 2002; 9(2)∶113.
- 7. Ichinose M, Liu XH, Hagihara N, et al.Extracellular bad fused to toxin transport domains induces apoptosis [J]. Cancer Res, 2002; 62(5)∶1433.
- 8. Kohler T, Schill C, Deininger MW, et al.High bad and bax mRNA expression correlate with negative outcome in acute myeloid leukemia (AML) [J]. Leukemia, 2002; 16(1)∶22.
- 9. Schimmer AD, Hedley DW, Pham NA, et al. BAD induces apoptosis in cells overexpressing Bcl2 or BclxL without loss of mitochondrial membrane potential [J]. Leuk Lymphoma,2001; 42(3)∶429.
- 10. Hattori T, Ookawa N, Fujita R, et al. Heterodimerization of bcl2 and bclx(L) with bax and bad in colorectal cancer [J]. Acta Oncol, 2000; 39(4)∶495.
