ObjectiveTo investigate the clinicopathological significance of integrin α5β1 expression and microvessel density(MVD) in gastric cancer(GC) and the correlation of MVD with integrin α5β1. MethodsThe expression of integrin α5β1 was detected by means of immunohistochemical staining (SP method) on paraffinembeded tissue specimens from 35 primary gastric carcinoma(PGC), 10 metastasic lymph node of gastric cancer and 8 chronic superficial gastritis (CSG). Vascular endothelial cells were stained immunohistochemically using antiCD34 monoclonal antibody to recognize microvessel(MV) in 35 cases of PGC and 8 CSG, MV was counted in 4 hot spot per slide under lightmicroscope (×400) and the average was defined as MVD. The results combined with clinicopathological parameters were analyzed statistically to characterize the role of integrin α5β1 and MVD in the progression of gastric cancer. ResultsIntegrin α5β1 expression and MVD in PGC were significantly higher than those in CSG respectively （t=3.32, P lt;0.01; t=2.30, P lt;0.05）; the expression of integrin α5β1 in PGC showed only a correlation with the invasion depth of tumor (t=2.29, P lt;0.05） while MVD showed all correlations with invasion depth,lymph node status and TNM stage （t=3.07, P lt;0.01; t=2.48, P lt;0.05; t=2.94,P lt;0.01）. Neither integrin α5β1expression nor MVD showed a relation with differential of PGC （t=0.15, P gt;0.05; t=0.41, P gt;0.05）. Integrin α5β1 was significantly overexpressed in lymph node metastatic cancer compared with that in corresponding PGC （t=2.45, P lt;0.05）; the difference of MVD showed no statistical significance among levels of integrin α5β1 expression in PGC （F =1.43,P＞0.05） and it showed no correlation with integrin α5β1 expression（r= 0.156, P=0.37）.Conclusion Overexpression of integrin α5β1 is present in GC and associates with the progression of tumor, implying that it may be viewed as the indicator of invasion and metastasis and the candidate target of gene therapy of gastric cancer. However, integrin α5β1 may not play an important role in the vascularization of GC.
Citation：
ZHOU Yejiang,SHI De,WU Bing.. Clinicopathological Significance of the Integrin α5β1 Expression and Microvessel Density in Gastric Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(3): 256-259. doi:
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- 3. 苏剑敏，王丽影，桂津，等. FAK和整合蛋白α5β1亚基在胃癌中表达的比较及其临床意义 [J]. 中华实验外科杂志，2001； 18（4）∶ 315.
- 4. Gong J, Wang D, Sun L, et al. Role of alpha 5 beta 1 integrin in determining malignant properties of colon carcinoma cells [J]. Cell Growth Differ, 1997； 8（1）∶ 83.
- 5. 方新初，苏剑敏，周国飞，等. 整合蛋白α5β1亚基的过表达对肝癌细胞粘附和迁移的影响 [J]. 中国癌症杂志，2001； 11(1)∶ 1.
- 6. 夏加增，朱正纲，燕敏，等. 胃癌血管生成及其与周围静脉血微转移的关系 [J]. 中华外科杂志， 2001； 39（7）∶ 511.
- 7. Senger DR, Claffey KP, Benes JB, et al. Angiogenesis promoted by vascular endothelial growth factor： regulation through α1β1 and α2β1 integrins [J]. Pro Natl Acad Sci USA, 1997； 94（25）∶ 13612.
- 8. Carron CP, Meyer DM, Pegg JA, et al. A peptidomimetic antagonist of the integrin αvβ3 inhibits leydig cell tumor growth and development of hypercalcemia of malignancy [J]. Cancer Res,1998； 58（9）∶ 1930.
- 9. Kim S, Bell K, Mousa SA, et al. Regulation of angiogenesis in vivo by ligation of integrin alpha 5 beta 1 with the central cellbinding domain of fibronectin [J]. Am J Patho, 2000； 156(4)∶ 1345.
