ObjectiveTo investigate the protective effect of SadenosylLmethionine on liver regeneration and
liver function in cirrhotic rats after hepatectomy. MethodsCirrhosis was successfully induced by injection of 40%
CCl4.Then,partial hepatectomy (about 30%) was performed in all rats. Cirrhotic rats were divided into 3
groups,namely,cirrhotic group (normal saline 5 ml/d,for 15 postoperative days，n=20),treatment group 1 〔S
adenosylLmethionine 10 mg/(kg·d),for 15 postoperative days,n=16〕 and treatment group 2 〔SadenosylL
methionine 20 mg/(kg·d),15 postoperative days,n=16〕,and normal control group was also established. Animals were
sacrificed at the 15th postoperative day and 30th postoperative day to take samples for detection of liver
function (Alb,ALT,TB,TBA) and serum TNFα.Liver tissues were also observed under light microscope and electron
microscope. ResultsIn two treatment groups,at the time point (15 postoperative days or 30 postoperative
days),concentrations of ALT,TB,TBA,Alb and TNFα were decreased significantly as compared with cirrhotic group (P
＜0.01),and concentration of Alb was increased significantly (P＜0.01).In contrast, there were no obvious difference
in the same time point of different dosetreatment groups (P gt;0.05),but the decrease of ALT,TB,TBA,TNFα and the
increase of Alb were more significant at the second time point (30th postoperative day) than the first time
point (15th postoperative day) when treated with same dose (P＜0.01).At the same time,concentration between TNF
α and ALT,TB,TBA showed a positive correlation (P＜0.01),and the concentration between TNFα and Alb showed a
negative correlation (P＜0.01).In addition, the histopathology showed SadenosylLmethionine had effects of
protecting liver function and enhancing liver regeneration. ConclusionThe study suggests that SadenosylL
methionine has the efficacy of enhancing liver regeneration and improving liver function.
Citation：
ZHANG Jie,LIAO Chuanwen,ZHU Hong,TIAN Daguang,GUO Yongzhang. An Observation of the Protective Effects of SAdenosylLMethionine on Liver Function in Cirrhotic Rats after Hepatectomy. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(4): 332-335. doi:
Copy
Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
| 1. |
2Higgins GM. Experimental pathology of liver restoration of the liver of the white rat following partial surgical
removal [Ｊ]. Aech Pathol, 1991; 12(2)∶186.
|
| 2. |
3Almasio P, Pagliaro L. Ademetionine: the state of the art and future prospects [Ｊ]. Ann Ital Med Int， 1993;
8(1 Suppl)∶52S.
|
| 3. |
4VarelaMoreiras G, AlonsoAperte E, Rubio M,et al. Carbon tetrachlorideinduced hepatic injury is associated
with global DNA hypomethylation and homocysteinemia: effect of Sadenosylmethionine treatment [Ｊ]. Hepatology,
1995; 22(4 Pt 1)∶1310.
|
| 4. |
5Holecek M, Skopec F, Sprongl L. Influence of buthionine sulfoximine, Sadenosylmethionine and glutathione on liver
regeneration following partial hepatectomy [Ｊ]. Arzneimittelforschung， 2000; 50(12)∶1093.
|
| 5. |
6Mato JM, Corrales FJ, Lu SC,et al. SAdenosylmethionine: a control switch that regulates liver function
[Ｊ].FASEB J, 2002; 16(1)∶15.
|
| 6. |
7Feo F,Garcea R,Daino L,et al. Early stimulation of polyamine biosynthesis during promotion by phenobartial of
diethylnitrosanineinduced rat liver carcinogenesis. The effect of variations of the SadenosylLmethionine
cellular pool [Ｊ].Carcinogenesis,1985; 6(12)∶1713.
|
| 7. |
8Lieber CS. Role of oxidative stress and antioxidant therapy in alcoholic and nonalcoholicliver diseases [Ｊ].
Adv Pharmacol， 1997; 38(8)∶601.
|
| 8. |
9Drahota Z, Rauchova H, Sedlak V, et al. The effect of triiodothyronine on changes of membrane fluidity in
regenerating rat liver [Ｊ]. Physiol Res， 1999; 48(2)∶167.
|
| 9. |
11Watson WH,Zhao Y,Chawla RK.Sadenosylmethionine attenuates the lipopolysaccharideinduced expression of the gene
for tumour necrosis alpha [Ｊ].Biochem J, 1999; 342(Pt 1)∶21.
|
| 10. |
孙自勤，王要军，权启镇，等. 肝纤维化大鼠血清有机磷酸酯酶的变化 [Ｊ].新消化病杂志， 1994; 2（4）∶206.
|
| 11. |
缪明永，蔡在龙，刘鹏飞,等.大鼠在肝再生过程中线粒体氧化磷酸化的调控 [Ｊ]. 中国病理生理杂志，1999; 15(5)∶398.
|
- 1. 2Higgins GM. Experimental pathology of liver restoration of the liver of the white rat following partial surgical
removal [Ｊ]. Aech Pathol, 1991; 12(2)∶186.
- 2. 3Almasio P, Pagliaro L. Ademetionine: the state of the art and future prospects [Ｊ]. Ann Ital Med Int， 1993;
8(1 Suppl)∶52S.
- 3. 4VarelaMoreiras G, AlonsoAperte E, Rubio M,et al. Carbon tetrachlorideinduced hepatic injury is associated
with global DNA hypomethylation and homocysteinemia: effect of Sadenosylmethionine treatment [Ｊ]. Hepatology,
1995; 22(4 Pt 1)∶1310.
- 4. 5Holecek M, Skopec F, Sprongl L. Influence of buthionine sulfoximine, Sadenosylmethionine and glutathione on liver
regeneration following partial hepatectomy [Ｊ]. Arzneimittelforschung， 2000; 50(12)∶1093.
- 5. 6Mato JM, Corrales FJ, Lu SC,et al. SAdenosylmethionine: a control switch that regulates liver function
[Ｊ].FASEB J, 2002; 16(1)∶15.
- 6. 7Feo F,Garcea R,Daino L,et al. Early stimulation of polyamine biosynthesis during promotion by phenobartial of
diethylnitrosanineinduced rat liver carcinogenesis. The effect of variations of the SadenosylLmethionine
cellular pool [Ｊ].Carcinogenesis,1985; 6(12)∶1713.
- 7. 8Lieber CS. Role of oxidative stress and antioxidant therapy in alcoholic and nonalcoholicliver diseases [Ｊ].
Adv Pharmacol， 1997; 38(8)∶601.
- 8. 9Drahota Z, Rauchova H, Sedlak V, et al. The effect of triiodothyronine on changes of membrane fluidity in
regenerating rat liver [Ｊ]. Physiol Res， 1999; 48(2)∶167.
- 9. 11Watson WH,Zhao Y,Chawla RK.Sadenosylmethionine attenuates the lipopolysaccharideinduced expression of the gene
for tumour necrosis alpha [Ｊ].Biochem J, 1999; 342(Pt 1)∶21.
- 10. 孙自勤，王要军，权启镇，等. 肝纤维化大鼠血清有机磷酸酯酶的变化 [Ｊ].新消化病杂志， 1994; 2（4）∶206.
- 11. 缪明永，蔡在龙，刘鹏飞,等.大鼠在肝再生过程中线粒体氧化磷酸化的调控 [Ｊ]. 中国病理生理杂志，1999; 15(5)∶398.
