In Vitro Effects of Recombinant Human Growth Hormone on Proliferation of Human Rectal Cancer Cell Line,HR8348 Cell_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

CHEN Jiayong , GAN Ping , LIANG Daoming , TAN Jing , ZHANG Yi

Department of General Surgery, The Second Affiliated Hospital of Kunming Medical College, Kunming 650101, China;

Corresponding author：

Keywords：

Human growth hormone,Rectal neoplasm,Cell culture,Cell cycle

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ObjectiveTo study the effects of recombinant human growth hormone (rhGH) on proliferation of human rectal cancer cell in vitro. MethodsThe experiment was divided into control group,rhGH group,Oxaliplatin (LOHP) group and rhGH+LOHP group. The double proliferation time of cells,cell inhibition rate,cell cycle, proliferation index (PI) and DNA inhibition rate of human rectal cancer line,HR8348,were studied by cell culture, MTT assay and flow cytometry on different concentration of rhGH. ResultsIn vitro the markedly accelerated effects of rhGH on multiplication of HR8348 cell line were not found: there was no statistical significance as compared rhGH group with control group or compared rhGH+LOHP group and LOHP group （P gt;0.05）. The double proliferation time of cells was markedly lengthened, cell inhibition rate and the cells arrested in G0-G1 phase were obviously increased, meanwhile, the cells in S phase （P＜0.05） and G2-M phase and PI were markedly decreased and DNA inhibition rate was obviously risen as compared rhGH+LOHP group with control group or rhGH+LOHP group and rhGH group （P＜0.01）.Conclusion
In vitro rhGH does not accelerate the multiplication of human rectal cancer cells.

Citation： CHEN Jiayong,GAN Ping,LIANG Daoming,TAN Jing,ZHANG Yi. In Vitro Effects of Recombinant Human Growth Hormone on Proliferation of Human Rectal Cancer Cell Line,HR8348 Cell. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(4): 362-365. doi: Copy

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1. 祁小平，黎介寿，陈彻. 人生长激素对癌细胞株周期动力学的影响 [Ｊ]. 肠内与肠外营养， 2000; 7（2）∶83.
2. Estrov Z, Meir R, Barak Y, et al. Human growth hormone and insulinlike growth factor1 enhance the proliferation of human leukemic blasts [Ｊ]. J Clin Oncol, 1991; 9(3)∶394.
3. Raff T, Germann G. Growth hormone in surgeryan assessment of current knowledge [Ｊ]. Chirurg, 1997; 68(10)∶995.
4. 陈嘉勇，张捷，谭晶，等. 胃、结肠癌患者术后生长激素应用的价值评估 [Ｊ]. 中国普外基础与临床杂志， 1999; 6（6）∶365.
5. OgilvyStuart AL, Shalet SM. Tumour occurrence and recurrence [Ｊ]. Horm Res, 1992; 38(Suppl 1)∶50.
6. Jauch KW, Heiss MM, Gruetzner U, et al. Prognostic significance of bone marrow micrometastases in patients with gastric cancer [Ｊ]. J Clin Oncol, 1996; 14(6)∶1810.
7. Heiss MM, Allgayer H, Gruetzner KU, et al. Individual development and uPAreceptor expression of disseminated tumour cells in bone marrow: a reference to early systemic disease in solid cancer [Ｊ]. Nat Med, 1995; 1(10)∶1035.
8. Fiebig HH, Dengler W, Hendriks HR. No evidence of tumor growth stimulation in human tumors in vitro following treatment with recombinant human growth hormone [Ｊ]. Anticancer Drugs, 2000; 11(8)∶659.
9. Bartlett DL, Stein TP, Torosian MH, et al. Effect of growth hormone and protein intake on tumor growth and host cachexia [Ｊ]. Surgery, 1995; 117(3)∶260.
10. Torosian MH，Donoway RB. Growth hormone inhibits tumors metastasis [Ｊ]. Cancer, 1991; 67(9)∶2280.
11. Wennbo H, GebreMedhin M, GritliLinde A, et al. Activation of the prolactin receptor but not the growth hormone receptor is important for induction of mammary tumors in transgenic mice [Ｊ]. J Clin Invest, 1997; 100(11)∶2744.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

In Vitro Effects of Recombinant Human Growth Hormone on Proliferation of Human Rectal Cancer Cell Line,HR8348 Cell

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