Experimental Study on Gut Mucosal CellMediated Immunity after Major Hepatectomy and Its Relation to Bacteria Translocation in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YANG Zhibin ¹ , ZHANG Bingyan ² , ZHU Hong ² , WANG Binghuang ² , GUO Yongzhang ²

Oncology Research Laboratory, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University, Shanghai 200438, China;

Corresponding author：

Keywords：

HepatectomyGut mucosaCellmediated immunityT lymphocyte

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ObjectiveTo investigate the change of cellmediated immunity in gut mucosa after major hepatectomy and to study its relationship with the bacteria translocation.MethodsFortyeight Spraguedawley adult male rats were randomly allocated into two groups, the sham operation group and the operation group. Besides without the hepatectomy, the sham operation group has the same course with the operation group. Seventy percent hepatectomy rats are divided as postoperative 6 h group (n=6)，12 h group (n=6)，24 h group (n=6) and 72 h group (n=6). Sixhour, 12hour, 24hour and 72hour after operation specimens were taken from jejunoileum respectively. Immunohistochemical staining was performed on frozen sections and image pattern analysis was used. We also investigate the change of liver function. ResultsTwentyfour hours and 72 hours after 70% hepatectomy, there was a significant reduction in the number of CD3+，CD4+and CD8+ T lymphocytes in the mucosal lamina propria of the operation group compared with the sham operation group （P lt;0.05）. There was significant difference between these two groups in liver function change （P lt;0.05）.ConclusionThere is an altered pattern of intestinal mucosal T lymphocytes after major hepatectomy, then the local cellmediated immunity was depressed, which may be the cause of translocation of enteric bacteria.

Citation： YANG Zhibin,ZHANG Bingyan,ZHU Hong,WANG Binghuang,GUO Yongzhang. Experimental Study on Gut Mucosal CellMediated Immunity after Major Hepatectomy and Its Relation to Bacteria Translocation in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(5): 469-470. doi: Copy

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1. Wang X, Andersson R， Soltesz V, et al. Bacterial translocation after major hepatectomy in patients and rats [Ｊ]. Arch Surg, 1992; 127(9)∶ 1101.
2. Osterberg J, Johnsson C, Gannedahl G, et al. Alterations in mucosal immune cell distribution in septic rats [Ｊ]. Shock, 1997; 7(3)∶ 182.
3. Emond J, CapronLaudereau M, Meriggi F， et al. Extent of hepatectomy in the rat: evaluation of basal condition and effect of therapy [Ｊ]. Eur Surg Res, 1989; 21(5)∶ 251.
4. Woodcock NP, Robertson J, Morgan DR, et al. Bacterial translocation and immunohistochemical measurement of gut immune function [Ｊ]. J Clin Pathol, 2001; 54(8)∶ 619.
5. Gautreaux MD, Deitch EA, Berg RD. T lymphocyte in the host defense against translocation from the gastrointestinal tract [Ｊ]. Infect immun, 1994; 62(7)∶ 2874.
6. Wang X, Andersson R, Soltesz V， et al. Effect of portal hypertension on bacterial translocation induced by major liver resection in rats [Ｊ]. Eur J Surg, 1993; 159(6-7)∶ 343.
7. Nishimura M, Fujiyama Y, Niwakawa M, et al. In vivo cytokine responses in gutassociated lymphoid tissue (GALT) and spleen following oral administration of staphylococcal enterotoxin B [Ｊ]. Immunol Lett, 2002; 81(1)∶ 77.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Experimental Study on Gut Mucosal CellMediated Immunity after Major Hepatectomy and Its Relation to Bacteria Translocation in Rats

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