The Effects of Somatostatin Analogue on Proliferation and Apoptosis of Human Cholangiocarcinoma Cell Line_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

CHEN Hua , HE Zhenping , MA Kuansheng , et al.

Department of Hepatobiliary Surgery, Southwest Hospital, The Third Military Medical College, Chongqing 430038;

Corresponding author：

Keywords：

Somatostatin Cell apoptosis Gene expression Human cholangiocarcinoma SKChA1 cell line

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Objective To investigate the regulatory effect of somatostatin analogue (SMS201995,SMS) on proliferation and apoptosis in human cholangiocarcinoma cell line in vitro. MethodsProliferation curve, flow cytometry, agarose gel electrophoresis, Annexin VFITC and flow cytometric immunofluorescent technique were performed to identify the inhibitory effect on cell proliferation and the induction of apoptosis of human cholangiocarcinoma cells (SKChA1). ResultsSMS significantly reduced the SKChA1 cell growth by serum in long experiments and transiently accumulated it in G0/G1 phase. Dotplot analysis of cells duallabeled with Annexin VFITC and PI confirmed the induction of apoptosis by SMS in SKChA1 cells.AnnexinVFITC labeling was markedly enhanced following treatment with SMS for 24 h. DNA of treated SKChA1 cells appeared a ladder pattern characteristic of apoptosis. Besides, timedependent increase in bax and decrease in bcl2 occured during SMS treatment. Conclusion SMS could inhibit the proliferation activity and induce apoptosis of cholangiocarcinoma cell line SKChA1. The mechanisms of apoptosis might be correlated with the expression of apoptosisregulatory gene bax and bcl2.

Citation： CHEN Hua,HE Zhenping,MA Kuansheng,et al.. The Effects of Somatostatin Analogue on Proliferation and Apoptosis of Human Cholangiocarcinoma Cell Line. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2002, 9(3): 164-167. doi: Copy

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1. 何双梧，赵言明，沈康强. 生长抑素对人结肠癌裸鼠移植瘤的作用及机理 [J].中国普外基础与临床杂志, 1999； 6（1）∶1.
2. Shih SC, Stutman O. Cell cycledependent tumor necrosis factor apoptosis [J]. Cancer Res,1996； 56(7)∶1591.
3. Teplan I. Peptides and antitumor activity. Development and investigation of some peptides with antitumor activity [J]. Acta Biol Hung， 2000； 51(1)∶1.
4. Dancygier H， Klein U， Leuschner U， et al. Somatostatincontaining cells in the extrahepatic biliary tract of humans [J]. Gastroenterology，1984； 86(5 Pt 1)∶892.
5. Pagliacci MC. Inhibition of human breast cancer cell (MCF7) growth in vitro by the somatostain analog SMS201995: effects on cell cycle parameters and apoptotic cell death [J]. Endocrinology, 1991； 129(5)∶2555.
6. Cheung NW, Boyages SC. Somatostatin14 and its analog octreotide exert a cytostatic effect on GH3 rat pituitary tumor cell proliferation via a transient G0/G1 cell cycle block [J].Endocrinology,1995； 136(10)∶4174.
7. 刘战培, 严律南. Bcl2蛋白在肝细胞肝癌发生过程中的表达及其与肝细胞凋亡变化的关系 [J]. 中国普外基础与临床杂志, 1999； 6(4)∶193.
8. Brinder C, Marx D, Binder L, et al. Expression of bax in relation to bcl2 and other predictive parameters in breast cancer [J].Ann Oncol,1996； 7(2)∶129.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

The Effects of Somatostatin Analogue on Proliferation and Apoptosis of Human Cholangiocarcinoma Cell Line

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