ObjectiveTo study the mechanism of reducing the intratumoral microvessel density (MVD) by Ginsenoside Rg3 (Rg3) combined with cytotoxic agent in xenotransplanted human breast infiltrating duct carcinoma in nude mice. MethodsSixteen female nude mice were randomly divided into 4 groups to receive cyclophosphamid (16 mg/kg,qd) combined with Rg3 (10 mg/kg, qd),Rg3(10 mg/kg,qd) alone,cyclophosphamid (16 mg/kg,qd) alone and 0.5% sodium carboxymethyl cellulose (0.5 ml,qd) respectively for 55 days. Breast cancer mass were weighed and sampled for light microscopic observation. The intratumor MVD was examined by immunohistochemical staining. ResultsThe tumor weight of treated group was significantly lower than that of control group. The tumor weight of the Rg3 combined with CTX group was lower than that of Rg3 group. The MVD value of Rg3 group was significantly lower than that of CTX group and control group. The MVD was significantly reduced in the Rg3 combined with CTX group than that in the others.ConclusionRg3 combined with CTX can inhibit the growth of xenotransplanted human breast infiltrating duct carcinoma, and reduce the intratumoral MVD.
Citation：
CHEN Dafu,ZHAO Yangbing,BAI Shaohuai,et al.. Effect of Ginsenoside Rg3 Combined with Cytotoxic Agent on the Progression of Xenotransplanted Human Breast Infiltrating Duct Carcinoma in Nude Mice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2002, 9(5): 300-302. doi:
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- 3. 白绍槐，赵扬冰，张旋波，等，人乳腺浸润性导管癌裸鼠移植模型的建立 \[J\].华西医科大学学报，1999； 30（2）∶220.
- 4. Gasparini G, Harris AL. Clinical importance of the determination of tumor angiogenesis in breast carcinoma: much more than a new prognostic tool \[J\]. J Clin Oncol,1995； 13（3）∶765.
- 5. Gasparini G, Biganzoli E， Bonoldi E， et al. Angiogenesis sustains tumor dormancy in patients with breast cancer treated with adjuvant chemotherapy \[J\]. Breast Cancer Res Treat， 2001； 65（1）∶71.
- 6. Teicher BA， Holden SA, Ara G, et al. Potentiation of cytotoxic cancer therapies by TNP470 alone and with other antiangiogenic agents \[J\]. Int J Cancer，1994； 57（6）∶920.
- 7. Kachel DL, Martin WJ 2nd. Cyclophosphamideinduced lung toxicity: mechanism of endothelial cell injury \[J\]. J Pharmacol Exp Ther, 1994; 268（1）∶42.
- 8. Brem H，Gresser I, Grosfeld J, et al. The combination of antiangiogenic agents to inhibit primary tumor growth and metastasis \[J\]. J Pediatr Surg, 1993； 28（10）∶1253.
