The Clinical Significance of Micrometastasis Detection in Sentinel Lymph Node of Breast Carcinoma_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

NIE Qiang , WU Chengyi.

Department of General Surgery, The First Affiliated Hospital of Chongqing University of Medical Science, Chongqing 400016;

Corresponding author：

Keywords：

Breast carcinoma Sentinel lymph node Micrometastasis Serial sectionImmuno histochemical staining

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ObjectiveTo study the detection methods of micrometastasis in sentinel lymph nodes (SLN) and their clinical significance. MethodsFifty women with breast carcinoma were included. SLN in fifty breast carcinoma was identified by using methylene blue staining to detect and remove them for routine hematoxylin and eosin stain and histological exam. All negative SLNs were examined by serial section (SS) with the section interval of 250 μm and HE stain for microscopic examination and immunohistochemical (IHC) exam was performed with CK19 monoclonal antibody. Then the above three detection methods were analyzed. All patients had axillary lymph node dissection (ALND)，and all none sentinel lymph nodes (NSLN) were examined by H amp;E staining.ResultsThe SLNs were identified in 45 of 50 patients with a detection rate of 90%. Sixteen SLNs were found positive with routine histological exam, the positive detecting rate was 35.56%, while the other 29 negative SLNs were found 7 and 6 cases of micrometastasis using SS and IHC methods，therefore the positive detecting rate was increased by 15.55% and 13.33%, respectively.Conclusion SS and IHC methods could detect the micrometastasis in negative SLN with routinely histological exam, increasing the positive detecting rate and decreasing the false negative rate.

Citation： NIE Qiang,WU Chengyi.. The Clinical Significance of Micrometastasis Detection in Sentinel Lymph Node of Breast Carcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2002, 9(5): 310-312. doi: Copy

1.	耿贯一主编. 流行病学 [M]. 第3版.北京：人民卫生出版社，1994∶72~80.
2.	Czerniecki BJ, Scheft AM, Callans LS,et al. Immunohistochemistry with pancytokeratins improves the sensitivity of sentinel lymph node biopsy in patients with breast carcinoma [J]. Cancer, 1999; 85(5)∶1098.
3.	Viale G, Bosari S,Mazzarol G, et al.Intraoperative examination of axillary sentinel lymph nodes in breast carcinoma patients [J]. Cancer,1999; 85(11)∶2433.
4.	McGuckin MA, Cummings MC, Walsh MD,et al. Occult axillary node metastasis in breast cancer,their detection and prognostic significance [J]. Br J Cancer, 1996; 73(1)∶88.
5.	Meyer JS. Sentinel lymph node biopsy strategies for pathologic examination of the specimen [J]. J Surg Oncol, 1998; 69(4)∶212.
6.	Dowlatshahi K, Fan M,Bloom KJ,et al. Occult metastasis in the sentinel lymph node of patients with early breast carcinoma. A preliminary study [J]. Cancer, 1999; 86(6)∶990.
7.	李志宇，赵扬冰. 腋窝前哨淋巴结活检在早期乳腺癌中的应用 [J]. 中国普外基础与临床杂志，1999； 6(5)∶317.
8.	Giuliano AE, Kirgan DM, Guenther JM, et al. Lymphatic mapping and sentinel lymphadenectomy for breast cancer [J]. Ann Surg, 1994; 220(3)∶391.
9.	Jamnink I, Fan M, Nagy S,et al. Serial sectioning of sentinel nodes in patients with breast cancer: a pilot study [J]. Ann Surg Oncol, 1998; 5(4)∶310.
10.	Turner RR, Ollila DW,Krasne DL, et al. Histopathologic validation of the sentinel lymph node hypothesis for breast carcinoma [J]. Ann Surg, 1997; 226(3)∶271.
11.	Noguchi S, Aihara T, Nakamori S,et al. The detection of breast carcinoma micrometastasis in axillary lymph nodes by means of reverse transcriptasepolymerase chain reaction [J]. Cancer, 1994; 74(5)∶1595.
12.	Nasser IA,Lee AK, Bosari S, et al. Occult axillary lymph node metastasis in “nodenegative” breast carcinoma [J]. Hum Pathol, 1993； 24(9)∶950.

1. 耿贯一主编. 流行病学 [M]. 第3版.北京：人民卫生出版社，1994∶72~80.
2. Czerniecki BJ, Scheft AM, Callans LS,et al. Immunohistochemistry with pancytokeratins improves the sensitivity of sentinel lymph node biopsy in patients with breast carcinoma [J]. Cancer, 1999; 85(5)∶1098.
3. Viale G, Bosari S,Mazzarol G, et al.Intraoperative examination of axillary sentinel lymph nodes in breast carcinoma patients [J]. Cancer,1999; 85(11)∶2433.
4. McGuckin MA, Cummings MC, Walsh MD,et al. Occult axillary node metastasis in breast cancer,their detection and prognostic significance [J]. Br J Cancer, 1996; 73(1)∶88.
5. Meyer JS. Sentinel lymph node biopsy strategies for pathologic examination of the specimen [J]. J Surg Oncol, 1998; 69(4)∶212.
6. Dowlatshahi K, Fan M,Bloom KJ,et al. Occult metastasis in the sentinel lymph node of patients with early breast carcinoma. A preliminary study [J]. Cancer, 1999; 86(6)∶990.
7. 李志宇，赵扬冰. 腋窝前哨淋巴结活检在早期乳腺癌中的应用 [J]. 中国普外基础与临床杂志，1999； 6(5)∶317.
8. Giuliano AE, Kirgan DM, Guenther JM, et al. Lymphatic mapping and sentinel lymphadenectomy for breast cancer [J]. Ann Surg, 1994; 220(3)∶391.
9. Jamnink I, Fan M, Nagy S,et al. Serial sectioning of sentinel nodes in patients with breast cancer: a pilot study [J]. Ann Surg Oncol, 1998; 5(4)∶310.
10. Turner RR, Ollila DW,Krasne DL, et al. Histopathologic validation of the sentinel lymph node hypothesis for breast carcinoma [J]. Ann Surg, 1997; 226(3)∶271.
11. Noguchi S, Aihara T, Nakamori S,et al. The detection of breast carcinoma micrometastasis in axillary lymph nodes by means of reverse transcriptasepolymerase chain reaction [J]. Cancer, 1994; 74(5)∶1595.
12. Nasser IA,Lee AK, Bosari S, et al. Occult axillary lymph node metastasis in “nodenegative” breast carcinoma [J]. Hum Pathol, 1993； 24(9)∶950.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

The Clinical Significance of Micrometastasis Detection in Sentinel Lymph Node of Breast Carcinoma

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