The Study of Blood Metastasis of Colorectal Cancer and Cancer Metastasis Related Factors_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

SUN Nianxu , WANG Dehu , TANG Bo.

Department of General Surgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038;

Corresponding author：

Keywords：

Colonic neoplasm Rectal neoplasm Metastasis factor

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Objective To study the relationship between blood metastasis of colorectal cancer and cancer metastasis related factors.MethodsCK20 mRNA in peripheral blood was investigated by reverse transcription polymerase chain reaction (RTPCR) and proteins of CD44v6 and p53 in cancer tissues were examined by immunohistochemical in 50 cases of colorectal neoplasm.　ResultsThe results showed that the positive rates of peripheral blood micrometastasis of colorectal cancer were 68%. It escalated along with the rising of the Dukes stage, the rates in Dukes C and D stage were significantly higher than that in Dukes A and B stage. The positive rates of CD44v6,p53 expression in colorectal cancer were 74% and 62% respectively. The positive rates of CD44v6 and p53 in Dukes A and B stage were significantly lower than those in Dukes C and D stage,in peripheral blood and colorectal cancer micrometastasispositive group were significantly higher than that in the micrometastasisnegative group. CK20 mRNA was significantly correlated with expressions of CD44v6 and p53 in cancer tissues. Conclusion The detection of CK20 mRNA in blood before operation and after operation examination of CD44v6 and p53 in cancer tissues are helpful for prediction of blood metastasis of colorectal neoplasm and postoperative treatment.

Citation： SUN Nianxu,WANG Dehu,TANG Bo.. The Study of Blood Metastasis of Colorectal Cancer and Cancer Metastasis Related Factors. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2002, 9(5): 348-351. doi: Copy

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1. 高进主编. 癌的侵袭与转移 [M].第1版. 北京：北京医科大学中国协和医科大学联合出版社，1996∶200～206.
2. 郭君其综述. 大肠癌微转移的研究现状及进展 [J].国外医学肿瘤学分册，1997； 24(5)∶281.
3. Garcia RL,Coltrera MD,Gown AM.Analysis of proliferative grade using antiPCNA/cyclin monoclonal antibodies in fixed,embedded tissues. Comparison with flow cytometric analysis [J]. Am J Pathol， 1989; 134(4)∶733.
4. 骆成玉，李世拥. 大肠癌患者门静脉血癌细胞的检测及临床意义 [J].中华外科杂志，1999； 37（4）∶214.
5. Hillaire S,Barbu V,Boucher E, et al. Albumin messenger RNA as a marker of circulating hepatocytes in hepatocellular carcinoma [J]. Gastroenterology, 1994; 106(1)∶239.
6. Moll R,Lowe A,Laufer J, et al. Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies [J]. Am J Pathol, 1992; 140(2)∶427.
7. Datta YH, Adams PT,Drobyski WR, et al. Sensitive detection of occult breast cancer by the reversetranscriptase polymerase chain reaction [J]. J Clin Oncol, 1994; 12(3)∶475.
8. Dammrich J, Vollmers HP, Heider KH, et al. Importance of different CD44v6 expression in human gastric intestinal and diffuse type cancers for metastatic lymphogenic spreading [J].J Mol Med, 1995; 73(8)∶395.
9. Herrlich P, Pals S, Ponta H. CD44 in colon cancer [J].Eur J Cancer， 1995; 31A(7-8)∶1110.
10. Rodriguez C, Monges G, Rouanet P, et al.CD44 expression patterns in breast and colon tumors: a PCRbased study of splice variants [J]. Int J Cancer, 1995; 64(5)∶347.
11. Gunthert U,Hofmann M, Rudy W, et al. A new variant of glycoprotein CD44 confers metastatic potential to rat carcinoma cells [J]. Cell, 1991； 65(1)∶13.
12. Seiter S, Arch R, Reber S, et al. Prevention of tumor metastasis formation by antivariant CD44 [J]. J Exp Med, 1993; 177(2)∶443.
13. Hollstein M, Sidransky D,Vogelstein B,et al. p53 mutations in human cancers [J]. Science, 1991; 253(5015)∶49.
14. Merritt AJ, Potten CS,Kemp CJ, et al. The role of p53 in spontaneous and radiationinduced apoptosis in the gastrointestinal tract of normal and p53deficient mice [J]. Cancer Res, 1994; 54(3)∶614.
15. 武忠弼主编. 病理学 [M].第4版.北京：人民卫生出版社，1997∶93～96.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

The Study of Blood Metastasis of Colorectal Cancer and Cancer Metastasis Related Factors

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