ObjectiveTo explore the effect of endotoxin on insulin secretion from islet βcell of rat pancreas.MethodsAfter the model of endotoxemia was established in rats with intraperitoneal injection of LPS (2 mg/kg),the changes of insulin level in the serum and pancreas were dynamically determined, the expression of inducible nitric oxide synthase (iNOS) by situ hybridization and DNA damage in islet cells were also observed, the effect of sodium nitroprusside (exogenous NO) on synthesis and secretion of insulin from isolated islet βcell of normal rat pancreas under high glucose stimulation was also evaluated.ResultsThe level of glucose and insulin in plasma were significantly increased at 12th and 6th h, respectively and kept on 3 d after injection of LPS,but the insulin level in pancreas was not remarkably altered.The expression of iNOS and DNA damaged significantly enhanced at 6 d after endotoxemia. The high glucosestimulated insulin synthesis and secretion were bly inhibited by exogenous NO.ConclusionThese findings suggest that LPS be stimulate the expression of iNOS and NO product,which inhibites synthesis and secretion of insulin in islet βcells,but it stimulates insulin secretion by another mechanism,and results in dysfunction and destruction of the rat pancreas.
Citation：
XU Linshui,LI Qiang.. Study of Dysfunction of Insulin Secretion in Islet βCells During Endoxemia in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2002, 9(6): 395-397. doi:
Copy
Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
| 1. |
俞志海,许霖水. 内毒素在烧伤大鼠胰岛素受体信号传导障碍中作用机制的研究 [Ｊ]. 第三军医大学学报,1999; 21(8)∶563.
|
| 2. |
李强,许霖水. NO对内毒素血症大鼠胰岛细胞的作用 [Ｊ]. 第三军医大学学报,1999; 21(1)∶42.
|
| 3. |
许霖水,王占科. 内毒素血症小鼠肝脏TNFα的表达及其意义 [Ｊ]. 中国普外基础与临床杂志,2000; 7(4)∶226.
|
| 4. |
蔡文琴,王泊云主编. 实用免疫细胞化学与核酸分子杂交技术 [M]. 成都: 四川科学技术出版社,1994∶406~412.
|
| 5. |
Ross GM, McMillan TJ, Wilcox P, et al.The single cell microgel electrophoresis assay (comet assay): technical aspects and application. Report on the 5th LH Gray Trust Workshop, Institute of Cancer Research, 1994[Ｊ]. Mut Res, 1995; 337(1)∶57.
|
| 6. |
Arnush M, Heitmeier MR, Scarim AL, et al. IL1 produced and released endogenously within human islets inhibits beta cell function [Ｊ]. J Clin Invest,1998; 102(3)∶516.
|
| 7. |
LeeperWoodford SK, Tobin BW. Tumor necrosis factor activity of pancreatic islets [Ｊ]. Am J Physiol,1997; 273(2 Pt 1)∶E433.
|
| 8. |
Arnush M, Scarim AL, Heitmeier MR,et al. Potential role of resident islet macrophage activation in the initiation of autoimmune diabetes [Ｊ]. J Immunol, 1998; 160(6)∶2684.
|
| 9. |
Yelich MR. Effects of endotoxin and interleukin1 on glucagon and insulin secretion from the perfused rat panereas [Ｊ]. Pancreas,1992; 7(3)∶358.
|
| 10. |
Elased KM, Furman BL. Acute streeinduced hyperinsulinemia in the pertussis toxintreated rat: possible role of humoral βcelltropic factors [Ｊ]. Metabolism,1994; 43(10)∶1221.
|
- 1. 俞志海,许霖水. 内毒素在烧伤大鼠胰岛素受体信号传导障碍中作用机制的研究 [Ｊ]. 第三军医大学学报,1999; 21(8)∶563.
- 2. 李强,许霖水. NO对内毒素血症大鼠胰岛细胞的作用 [Ｊ]. 第三军医大学学报,1999; 21(1)∶42.
- 3. 许霖水,王占科. 内毒素血症小鼠肝脏TNFα的表达及其意义 [Ｊ]. 中国普外基础与临床杂志,2000; 7(4)∶226.
- 4. 蔡文琴,王泊云主编. 实用免疫细胞化学与核酸分子杂交技术 [M]. 成都: 四川科学技术出版社,1994∶406~412.
- 5. Ross GM, McMillan TJ, Wilcox P, et al.The single cell microgel electrophoresis assay (comet assay): technical aspects and application. Report on the 5th LH Gray Trust Workshop, Institute of Cancer Research, 1994[Ｊ]. Mut Res, 1995; 337(1)∶57.
- 6. Arnush M, Heitmeier MR, Scarim AL, et al. IL1 produced and released endogenously within human islets inhibits beta cell function [Ｊ]. J Clin Invest,1998; 102(3)∶516.
- 7. LeeperWoodford SK, Tobin BW. Tumor necrosis factor activity of pancreatic islets [Ｊ]. Am J Physiol,1997; 273(2 Pt 1)∶E433.
- 8. Arnush M, Scarim AL, Heitmeier MR,et al. Potential role of resident islet macrophage activation in the initiation of autoimmune diabetes [Ｊ]. J Immunol, 1998; 160(6)∶2684.
- 9. Yelich MR. Effects of endotoxin and interleukin1 on glucagon and insulin secretion from the perfused rat panereas [Ｊ]. Pancreas,1992; 7(3)∶358.
- 10. Elased KM, Furman BL. Acute streeinduced hyperinsulinemia in the pertussis toxintreated rat: possible role of humoral βcelltropic factors [Ｊ]. Metabolism,1994; 43(10)∶1221.
