Objective To investigate the pathological characteristics of hepatic energy metabolism changes following hepatic inflow occlusion and the tolerant limit to ischemia in cholestatic rats.
Methods On the day 7 after rats biliary obstruction, the survival rate, hepatic mitochondrial respiratory function, content of ATP, and the ketone body ratio in arterial blood were investigated following the different duration of hepatic ischemia and reperfusion with portal blood bypass.
Results The survival rate on postoperative day 10 was 100%, 100% and 40% subjected to 30, 60 and 90min of hepatic vascular occlusion. The hepatic energy metabolic function was decreased markedly following ischemia, and was increased markedly on 24 hours following reperfusion subjected to 30, 60min of hepatic vascular occlusion, but it had less increase with 90min of hepatic vascular occlusion.
Conclusion The hepatic energy metabolic function injury is reversible in cholestatic rats, and the rats can tolerate hepatic inflow occlusion within 60min, but the hepatic energy metabolic function injury is irreversible after 90min of hepatic occlusion.
Citation：
ZHANG Leida,DONG Jiahong,HAN Benli,et al.. THE EFFECT OF HEPATIC INFLOW OCCLUSION ON HEPATIC ENERGY METABOLISM IN CHOLESTATIC RATS. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2001, 8(1): 12-14. doi:
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- 1. Yamamoto S, Nitta N, Kamiyama Y, et al. Effects of partial deprivation of portal blood on arterial blood ketone body ratio in rabbits [J]. Eur Surg Res, 1989; 21(1)∶98.
- 2. 陈孝平，吴在德，裘法祖.家兔常温下阻断肝门最大耐受时限的实验研究 [J].中华外科杂志，1984； 22(3)∶153.
- 3. 何效东.大鼠门静脉转流下耐受入肝血流阻断安全时限的实验研究[D].第三军医大学硕士研究生论文，1998∶1～46.
- 4. Johnson D.Isolation of liver or kidney mitochondria [J].Methods in Enzymology, 1967; 10(1)∶94.
- 5. Estabrook RW. Mitochondrial respiratory control and the polarographic measurement of ADP/O ratio [J].Methods In Enzymology, 1967; 10(1)∶41.
- 6. 严涛，张平，段恒春等.组织ATP、ADP、AMP水平的荧光素酶系测定 [J].第三军医大学学报，1987； 9(3)∶294.
- 7. Williamson DH, Mellanby J. βHydroxybutyrate. In:Edited by HV Bergmeyer. Methods of enzymatic analysis [M]. New York:Academic press, In C. 1974∶18361839.
- 8. Mellanby J, Williamson DH. Acetoacetate. In:Edited by HV Bergmeyer. Methods of enzymatic analysis[M].New York:Academic press,In C. 1974∶1446-1451.
