EFFECTS OF PHOSPHOROTHIOATE ANTISENSE OLIGONUCLEOTIDES TARGETING MRP mRNA ON DRUGRESISTANT CELL LINE SMMC-7721／ADM_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

Department of General Surgery, First Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041;

Corresponding author：

Keywords：

Phosphorothioate antisense oligonucleotides Multidrug resistance Human hepatocellular carcinoma cell

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the effect of phosphorothioate antisense oligonucleotides(AS-ODN) on suppressing multidrug resistance-associated protein gene(MRP) in human drug-resistant hepatocellular carcinoma cell line (SMMC-7721/ADM).
Methods Cell line was transfected with a synthetic S-ODN complementary to the coding region of MRP mRNA, Lipofectamine acting as carrier. The drug sensitivity was measured by MTT assay. The expression of MRP mRNA was detected by RT-PCR and the expression of P190 was detected by flow cytometry.
Results AS-ODN inhibited expression of MRP mRNA and P190 and promoted sensitivity to daunorubicinum and adriamycinum.
Conclusion AS-ODN can reduce the expression of MRP gene. MDR caused by MRP is an important cause of multidrug resistance of SMMC-7721/ADM.

Citation： YANG Jiayin,LUO Huayou,LIN Qiyuan,et al.. EFFECTS OF PHOSPHOROTHIOATE ANTISENSE OLIGONUCLEOTIDES TARGETING MRP mRNA ON DRUGRESISTANT CELL LINE SMMC-7721／ADM. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2001, 8(3): 141-144. doi: Copy

1.	Cole SP, Bhardwaj G, Gerlach JH, et al. Overexpression of transporter gene in a multidrugresistant human lung cancer cell line 〔J〕. Science, 1992； 258(5088)∶1650.
2.	Stewart AJ, Canitrot Y, Banacchini E, et al. Reduction of expression of the multidrug resistance protein(MRP) in human tumor cells by antisense phosphorothioate oligonucleotides 〔J〕. Biochem Pharm, 1996； 51(4)∶461.
3.	刘炳仁，杨纯正，栾凤君等.用逆转录多聚酶链反应检测白血病患者多药耐药基因的表达〔J〕.中华血液学杂志，1995； 16(2)∶65.
4.	Sargent JM, Taylor CG. Appraisal of the MTT assay as a rapid test of chemosensitivity in acute myeioid lekaemia 〔J〕. Br J Cancer, 1989； 60(2)∶206.
5.	Nakamura H, Oda Y, Iwai S, et al. How does Rnase H recognize a DNARNA hybrid 〔J〕? Pro Natl Acad Sci USA, 1991； 88(24)∶11535.
6.	Helene C, Toulme JJ. Specific regulation of gene expression by antisense, sense and antigene nucleic acids 〔J〕. Biochem Biophys Acta, 1990； 1049(2)∶99.
7.	Carter G, Lemoine NR. Antisense technology for cancer therapy: does it make sense 〔J〕? Br J Cancer, 1993； 67(5)∶869.
8.	Richert ND, Aldwin L, Nitesci D, et al. Stability and covalent modification of Pglycoprotein in multidrug resistant KB cells 〔J〕. Biochemistry, 1988； 27(20)∶7607.

1. Cole SP, Bhardwaj G, Gerlach JH, et al. Overexpression of transporter gene in a multidrugresistant human lung cancer cell line 〔J〕. Science, 1992； 258(5088)∶1650.
2. Stewart AJ, Canitrot Y, Banacchini E, et al. Reduction of expression of the multidrug resistance protein(MRP) in human tumor cells by antisense phosphorothioate oligonucleotides 〔J〕. Biochem Pharm, 1996； 51(4)∶461.
3. 刘炳仁，杨纯正，栾凤君等.用逆转录多聚酶链反应检测白血病患者多药耐药基因的表达〔J〕.中华血液学杂志，1995； 16(2)∶65.
4. Sargent JM, Taylor CG. Appraisal of the MTT assay as a rapid test of chemosensitivity in acute myeioid lekaemia 〔J〕. Br J Cancer, 1989； 60(2)∶206.
5. Nakamura H, Oda Y, Iwai S, et al. How does Rnase H recognize a DNARNA hybrid 〔J〕? Pro Natl Acad Sci USA, 1991； 88(24)∶11535.
6. Helene C, Toulme JJ. Specific regulation of gene expression by antisense, sense and antigene nucleic acids 〔J〕. Biochem Biophys Acta, 1990； 1049(2)∶99.
7. Carter G, Lemoine NR. Antisense technology for cancer therapy: does it make sense 〔J〕? Br J Cancer, 1993； 67(5)∶869.
8. Richert ND, Aldwin L, Nitesci D, et al. Stability and covalent modification of Pglycoprotein in multidrug resistant KB cells 〔J〕. Biochemistry, 1988； 27(20)∶7607.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

EFFECTS OF PHOSPHOROTHIOATE ANTISENSE OLIGONUCLEOTIDES TARGETING MRP mRNA ON DRUGRESISTANT CELL LINE SMMC-7721／ADM

Abstract Full text Figures/Tables Video References Cited by

Format

Content