EFFECTS OF ARSENIC TRIOXIDE ON HEPATOMA CELL LINE BEL-7402_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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Department of General Surgery,The First Affiliated Hospital,Harbin University of Medical Sciences,Harbin 150001;

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Hepatocellular carcinoma Apoptosis Arsenic trioxide

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Objective To observe the effect of a wide range of concentration of arsenic trioxide on hepatoma cell line BEL-7402 with variable duration. Methods The cell activity and morphologic changes were studied after treated with different concentration. The apoptosis were detected by flow cytometry assay and DNA Gel electrophoresis. Results The effect of arsenic trioxide on hepatoma cell lines were dependent on the time and concentration obviously. Hepatoma cells cultured with different concentration presented apoptosis features: i.e. intact cell membrane, chromatin condensation, nucleic fragmentation and apoptotic body formation; flow cytometry analysis showed an arrestment at G2/M phase and a subG1 cell peak, DNA gel electrophoresis showed a marked DNA ladder. Conclusion Arsenic trioxide can obviously inhibit the growth of hepatoma cell lines through inducing hepatoma cell apoptosis.

Citation： LIU Lianxin,ZHU Anlong,JIANG Hongchi,et al.. EFFECTS OF ARSENIC TRIOXIDE ON HEPATOMA CELL LINE BEL-7402. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2001, 8(4): 209-211下转217. doi: Copy

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1. 倪建华, 陈国强. 砷的生物学作用及其在临床上的应用前景〔Ｊ〕.国外医学输血及血液学分册, 1997; 20(4)∶210.
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3. 张鹏, 王树叶, 胡龙虎等. 三氧化二砷注射液治疗72例急性早幼粒细胞性白血病〔Ｊ〕. 中华血液学杂志, 1996; 17(1)∶58.
4. 邓友平, 林晨, 梁萧等. 三氧化二砷诱导人宫颈癌HeLa细胞凋亡及bcl2保护作用的机制研究〔Ｊ〕. 中国科学C辑, 1999; 29(4)∶426.
5. 郁宝铭, 崔巍, 陆爱国等. 三氧化二砷对结肠癌细胞抑制作用的实验研究〔Ｊ〕. 肿瘤, 1999; 19(5)∶289.
6. 陈其奎, 袁世珍, 黄世清等. 三氧化二砷诱导胰腺癌细胞周期阻滞与凋亡作用〔Ｊ〕. 中华医学杂志, 1998; 78(8)∶578.
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8. 唐韦, 陈国强, 史桂英等. 三氧化二砷对急性早幼粒细胞性白血病细胞株的双重效应研究〔Ｊ〕. 中华医学杂志, 1997; 77(8)∶509.
9. Wenlin S, Micro F, Fabiana F. Arsenic trioxide as an inducer of apoptosis and loss of PML/RAR α protein in acute promyelocytic leukemia cells 〔Ｊ〕. Natl Cancer Institute, 1998; 90(2)∶124.
10. 王振义. 开展砷剂治疗白血病的临床和机制研究〔Ｊ〕. 中华血液学杂志, 1996; 17(1)∶57.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

EFFECTS OF ARSENIC TRIOXIDE ON HEPATOMA CELL LINE BEL-7402

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