Objective To investigate the reversal effect of antisense phosphorothioate oligonucleotide （ASOND) on human hepatoma resistant cells.
Methods Human hepatoma resistant cells SMMC-7721 was transfected with synthetic antisense phosphorothioate oligonucleotide complementary to the 5′ region flanking the AUG initiation codon mediated by lipofectamine. In vitro drug sensitivity was measured by MTT assay. The expression of P-170 was determined by flow cytometry and mRNA was assessed by RT-PCR.
Results ASOND inhibited the expression of mRNA and p-170 in SMMC-7721, enhanced the sensitivity of SMMC-7721 to chemotherapeutic drug. The best inhibitory effect was achived by the dose of 0.5μmol/L.
Conclusion ASOND enhanced the sensitivity of SMMC-7721 to chemotherapeutic drug and reversed the multidrug resistance of SMMC-7721 partially.
Citation：
LUO Huayou,YANG Jiayin,LIN Qiyuan,et al.. REVERSAL OF MULTIDRUG RESISTANCE OF HUMAN HEPATOMA RESISTANT CELLS IN VITRO BY ANTISENSE PHOSPHOROTHIOATE OLIGONUCLEOTIDE. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2001, 8(4): 220-222. doi:
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| 1. |
Christensen JG, LeBlanc GA. Reversal of multidrug resistance in vivo by dietary administration of the photochemical indole3carbinol 〔Ｊ〕. Cancer Res, 1996; 56(3)∶574.
|
| 2. |
Alahari SK, Dean MH, Fisher R, et al. Inhition of expression of the multidrug resistance associated Pglycoprotein by phosphorothioate and 5′cholesterolconjugated phosphorothioate antisense oligonucleotides 〔Ｊ〕. Molecular Pharmacology, 1996; 50(4)∶808.
|
| 3. |
Noonan KE, Beck C, Holzmayer TA, et al. Quantitative analysis of MDR1 (multidrug resistance) gene expression in human tumors by polymerase chain reaction 〔Ｊ〕. Proc Natl Acad Sci USA, 1990; 87(18)∶7160.
|
| 4. |
Sargent JM, Taylor CG. Appraisal of the MTT assay as a rapid test of chemosensitivity in acute myeioid lekaemia 〔Ｊ〕. Br J Cancer, 1989; 60(2)∶206.
|
| 5. |
Roninson IB. The role of MDR1(Pglycoprotein) gene in multidrug resistance in vitro and in vivo 〔Ｊ〕. Biochem Pharmacol, 1992; 43(1)∶95.
|
| 6. |
Behr JP, Demeneix B, Loeffler JP, et al. Efficient gene transfer into mammalian primary endocrine cells with lipopolyaminecoated DNA 〔Ｊ〕. Proc Natl Acad Sci USA, 1989; 86(18)∶6982.
|
| 7. |
Wagner RW. Gene inhibition using antisense oligodeoxynuxleotides 〔Ｊ〕. Nature, 1994; 372(6504)∶333.
|
| 8. |
Richert ND, Laldwin D, Nilescki MM, et al. Stability and covalent modification of Pglycoprotein in multidrug resistance KB cells 〔Ｊ〕. Biochemistry, 1988; 27(20)∶7607.
|
| 9. |
Walder RY, Walder JA. Role of Rnase H in hybridarrested translation by antisense oligonucleotides 〔Ｊ〕. Proc Natl Acad Sci USA, 1988; 85(14)∶5011.
|
| 10. |
Kulka M, Simith CC, Aurelian R, et al. Site specificity of the inhibitory effects of oligo (nucleotide methylphonate)s complementary to the acceptor splice junction of herpes simplex virus type1 immediate early mRNA 〔Ｊ〕. Proc Natl Acad Sci USA, 1989; 86(18)∶6868.
|
| 11. |
Boiziau C, Kurfurst C, Cazenave V, et al. Inhibition of translation initiation by antisense oligonucleotides via an RnaseH independent mechanism 〔Ｊ〕. Nucleic Acids Res, 1991; 19(5)∶1113.
|
- 1. Christensen JG, LeBlanc GA. Reversal of multidrug resistance in vivo by dietary administration of the photochemical indole3carbinol 〔Ｊ〕. Cancer Res, 1996; 56(3)∶574.
- 2. Alahari SK, Dean MH, Fisher R, et al. Inhition of expression of the multidrug resistance associated Pglycoprotein by phosphorothioate and 5′cholesterolconjugated phosphorothioate antisense oligonucleotides 〔Ｊ〕. Molecular Pharmacology, 1996; 50(4)∶808.
- 3. Noonan KE, Beck C, Holzmayer TA, et al. Quantitative analysis of MDR1 (multidrug resistance) gene expression in human tumors by polymerase chain reaction 〔Ｊ〕. Proc Natl Acad Sci USA, 1990; 87(18)∶7160.
- 4. Sargent JM, Taylor CG. Appraisal of the MTT assay as a rapid test of chemosensitivity in acute myeioid lekaemia 〔Ｊ〕. Br J Cancer, 1989; 60(2)∶206.
- 5. Roninson IB. The role of MDR1(Pglycoprotein) gene in multidrug resistance in vitro and in vivo 〔Ｊ〕. Biochem Pharmacol, 1992; 43(1)∶95.
- 6. Behr JP, Demeneix B, Loeffler JP, et al. Efficient gene transfer into mammalian primary endocrine cells with lipopolyaminecoated DNA 〔Ｊ〕. Proc Natl Acad Sci USA, 1989; 86(18)∶6982.
- 7. Wagner RW. Gene inhibition using antisense oligodeoxynuxleotides 〔Ｊ〕. Nature, 1994; 372(6504)∶333.
- 8. Richert ND, Laldwin D, Nilescki MM, et al. Stability and covalent modification of Pglycoprotein in multidrug resistance KB cells 〔Ｊ〕. Biochemistry, 1988; 27(20)∶7607.
- 9. Walder RY, Walder JA. Role of Rnase H in hybridarrested translation by antisense oligonucleotides 〔Ｊ〕. Proc Natl Acad Sci USA, 1988; 85(14)∶5011.
- 10. Kulka M, Simith CC, Aurelian R, et al. Site specificity of the inhibitory effects of oligo (nucleotide methylphonate)s complementary to the acceptor splice junction of herpes simplex virus type1 immediate early mRNA 〔Ｊ〕. Proc Natl Acad Sci USA, 1989; 86(18)∶6868.
- 11. Boiziau C, Kurfurst C, Cazenave V, et al. Inhibition of translation initiation by antisense oligonucleotides via an RnaseH independent mechanism 〔Ｊ〕. Nucleic Acids Res, 1991; 19(5)∶1113.
