【Abstract】Objective To study the mechanisms of enhancing effect of mild hypothermia (MH) to ischemic preconditioning (IP) on hepatic ischemiareperfusion (I-R) injury. Methods To observe the content of the marker enzymes of liver damage (ALT,AST,LDH) and malondialdehyde (MDA), and activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPX), total antioxidase (TAX) in inferior vena cava blood above liver in nonischemic control group (n=6), I-R group (n=6), IP group (n=6) and mild hypothermic ischemic preconditioning (MHIP) group (n=6). Results After I-R the content of ALT,AST, LDH and MDA were significantly elevated (P<0.01), SOD,CAT,GSH-PX,ACT activities were declined obviously (P<0.01). The content of ALT,AST,LDH and MDA were significantly lower in IP group than those in I-R group, and in MHIP group than those in IP group (P<0.01,P<0.05), and the content of SOD, CAT,GSH-PX, ACT activities were significantly higher in IP group than those in I-R group, and in MHIP group than those in IP group (P<0.01,P<0.05). Conclusion Ischemic preconditioning may enhance the oxidation-resistance of liver, and reduce the oxygen free radical injury to liver after ischemia-reperfusion. Mild hypothermia may enhance the protective effect of IP on hepatic ischemiareperfusion injury.
Citation:
HE Jinsong,ZHU Wenfeng,JIANG Lihua,et al.. ENHANCE EFFECT OF MILD HYPOTHERMIA TO ISCHEMIC PRECONDITIONING ON LIVER INJURY OF HEPATIC ISCHEMIAREPERFUSION. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2001, 8(6): 370-372. doi:
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- 1. Parratt JR. Protection of the heat by ischemic preconditioning: mechanisms and possibilities for pharmacological ploitation 〔J〕. Trends Pharmacol Sci, 1994; 15(1)∶19.
- 2. 陈莹莹,鲁巍峰. 缺血预处理的心肌保护作用机制及其临床意义 〔J〕. 心血管病学进展, 1997; 18(5)∶271.
- 3. 刘秀华, 庞永政,陈魁等. 预处理对大鼠小肠缺血再灌注损伤的保护作用 〔J〕. 北京医科大学学报, 1996; 28(1)∶20.
- 4. 何劲松,姜立华, 吕新生等. 缺血预处理对肝缺血再灌注后氧自由基损伤的保护作用 〔J〕. 现代临床普通外科杂志, 2000; 5(2)∶88.
- 5. 朱彤,叶启发,陈实等. 缺血预处理对肝脏缺血再灌注损伤的保护作用的实验研究 〔J〕. 肝胆外科杂志, 1999; 7(3)∶222.
- 6. Murry BS. Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardial 〔J〕. Ciculation, 1986; 74(5)∶1124.
- 7. Li YW. The transient nature of the effect of ischemic preconditioning on myocardial infarct size and ventricular arrhrthmia 〔J〕. Am Heart J, 1992; 123(2)∶346.
- 8. 郭曲练,潭秀娟,蔡宏伟等. 亚低温对完全性脑缺血再灌注后丙二醛含量和超氧化物歧化酶的影响 〔J〕. 中华创伤杂志, 1997; 13(1)∶10.
- 9. 何劲松, 姜立华, 扬新等. 亚低温对肝缺血再灌注后氧自由基损伤的保护作用 〔J〕. 中国普通外科杂志, 2000; 9(2)∶132.
- 10. 皮业庆. 肝缺血再灌注损伤模型. 见: 汪谦主编. 现代医学实验方法 〔M〕. 北京: 人民卫生出版社, 1997∶909~910.
- 11. Marklund S. Distribution of Cu, ZnSOD in human tissue 〔J〕. Acta Physiol Scand (Suppl), 1980; 492(1)∶19.
- 12. 尤家禄. 自由基与肿瘤. 见: 潘世坤,罗正曜主编. 病理生理学进展(三) 〔M〕. 北京: 人民卫生出版社, 1987∶97~103.
- 13. Hiroyuki R. Mechanism and prevention of ischemiareperfusioninduced liver injury in rats 〔J〕. Surg Res, 1991; 51(2)∶240.
- 14. 王文杰. 超氧自由基与超氧化物歧化酶 〔J〕. 生理科学进展, 1985; 16(3)∶196.