【Abstract】Objective To investigate whether liver resection for hepatocellular carcinoma (HCC) causes dissemination of liver tumor cells into blood circulation. Methods Fourteen patients with HCC, but without evidences of metastasis, were enrolled for the study. Blood samples of peripheral blood before skin incision and after abdominal wall suture, and of hepatic venous blood and portal venous blood after liver parenchyma dissection, were obtained. AFPmRNA was detected by reverse transcription polymerase chain reaction assays, the change of the level of its expression during operation was assessed by semi-quantitative analysis. Results The rate of its expression before and after operation in peripheral blood, and during operation in portal venous blood and in hepatic venous was 42.9%, 35.7%, 42.9% and 57.1% respectively. There were no differences between them. However, the level of its expression in hepatic venous blood was significantly higher than others (P＜0.05). Conclusion Liver resection for HCC induces releases of cells from the liver, probably including tumor cells, into blood circulation.
Citation：
ZHAO Hong,CHEN Xiaoping,ZHAO Xiping,et al.. THE ALTERNATION OF AFP-mRNA DETECTED IN BLOOD DURING LIVER RESECTION FOR HEPATOCELLULAR CARCINOMA AND ITS SIGNIFICANCE. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2001, 8(6): 403-405. doi:
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Moreno JG, Groce CM, Fischer R, et al. Detection of hematogenous micrometastasis in patients with prostate cancer 〔Ｊ〕. Cancer Res, 1992; 52(21)∶6110.
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Louha M, Poussin K, Ganne N, et al. Spontaneous and latrogenic sprending of liverderived cells into peripheral blood of patients with primary liver cancer 〔Ｊ〕. Hepatology, 1997; 26(4)∶998.
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Naoto O, Akimasa N, Shin T, et al. Clinical significance of αfetoprotein mRNA during perioperative period in HCC 〔Ｊ〕. Hepatogastrienterology, 1999; 46(25)∶381.
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Malek L, Jerome N, Herve Z, et al. Liver resection and needle biopsy cause hematogenous dissemination of liver cells 〔Ｊ〕. Hepatology, 1999; 29(4)∶ 879.
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Lemoine A, Le Bricon T, Salvucci M, et al. Prospective evaluation of circulating hepatocytes by alphafetoprotein mRNA in humans during liver surgery 〔Ｊ〕. Ann Surg, 1997; 226(1)∶43.
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- 1. Smith B, Selby P, Southgate J, et al. Detection of melanoma cells in peripheral blood by means of reverse transcriptase and polymerase chain reaction 〔Ｊ〕. Lancet, 1991; 338(8777)∶1227.
- 2. Naito H, Kuzumaki N, Uchino J, et al. Detection of tyrosine hydroxylase mRNA and minimal neuroblastoma cells by the reverse transcriptionpolymerase chain reaction 〔Ｊ〕. Eur J Cancer, 1991; 27(6)∶762.
- 3. Carr BI, Kar S. An assay for hepatoma micrometastasis: albumin gene expression in peripheral blood from patients with advanced hepatocellular carcimona (HCC) 〔Ｊ〕. Hepatology, 1991; 14(1)∶108A.
- 4. Brown DC, Purushotham AD, Birnie GD, et al. Detection of intraoperative tumor cell dissemination in patients with breast cancer by use of reverse transcription and polymerase chain reaction 〔Ｊ〕. Surgery, 1995; 117(1)∶96.
- 5. Glaves D, Huben RP, Weis L. Hematogenous dissemination of cells from human renal adenocarcinomas 〔Ｊ〕. Br J Cancer, 1988; 57(1)∶32.
- 6. Leather AJ, Gallegos NC, Kocjan G, et al. Detection and numeration of circulating tumor cells in colorectal cancer 〔Ｊ〕. Br J Surg, 1993; 80(6)∶777.
- 7. Moreno JG, Groce CM, Fischer R, et al. Detection of hematogenous micrometastasis in patients with prostate cancer 〔Ｊ〕. Cancer Res, 1992; 52(21)∶6110.
- 8. Louha M, Poussin K, Ganne N, et al. Spontaneous and latrogenic sprending of liverderived cells into peripheral blood of patients with primary liver cancer 〔Ｊ〕. Hepatology, 1997; 26(4)∶998.
- 9. Naoto O, Akimasa N, Shin T, et al. Clinical significance of αfetoprotein mRNA during perioperative period in HCC 〔Ｊ〕. Hepatogastrienterology, 1999; 46(25)∶381.
- 10. Malek L, Jerome N, Herve Z, et al. Liver resection and needle biopsy cause hematogenous dissemination of liver cells 〔Ｊ〕. Hepatology, 1999; 29(4)∶ 879.
- 11. Lemoine A, Le Bricon T, Salvucci M, et al. Prospective evaluation of circulating hepatocytes by alphafetoprotein mRNA in humans during liver surgery 〔Ｊ〕. Ann Surg, 1997; 226(1)∶43.
- 12. Esteban C, Trojan J, Macho A, et al. Activation of an alphafetoprotein or receptor pathway in human normal and malignant peripheral blood mononuclear cells 〔Ｊ〕. Leukemia，1993; 7(11)∶1807.
