To observe the change in plasma endotoxin and cytokine during the early period of intra-abdominal infection (IAI) complicated by multiple system organ dysfunction (MSOD) in animals. Twenty rabbits were randomly divided in to two groups. One group received the operation of cecal ligation plus puncture (CLP) inducing IAI complicated by MSOD, and another group received sham operation as a control. All animals were placed in metabolic cages and maintained with intravenous infusion for one week. Plasma levels of endotoxin and cytokine (TNF, IL-1, IL-6) were determined seperately at the beginning (0 hour) or 1, 2, 3, 4, 5, 6 and 24 hours after CLP. Blood bacteria cultures and pathological examination of several organs were made when the animal was dead or killed. Results: The levels of plasma endotoxin, TNF and IL-6 were found to be significantly increased at one or two hours after CLP, the incidence rate of bacteriemia was 80% and the pathological alterations in the abdomen and organs were remarkale, with an average survival time of 84.1±39.0 hours in CLP group. No change in plasma IL-1 level was found in the CLP group. Conclusion: The plasma levels of endotoxin and cytokine (TNF and IL-6) do increase in the early period of IAI complicated by MSOD, and the change in plasma IL-1 is not obvious.
Citation：
Quan Zhufu,Li Jieshou,Zang Jing,et al.. EARLY CHANGE IN PLASMA ENDOTOXIN AND CYTOKINE RELATIING TO INTRA-ABDOMINAL INFECTION COMPLICATED BY MULTIPLE SYSTEM ORGAN DYSFUNCTION. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1999, 6(2): 73-75. doi:
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- 1. Davies MG, Hagen PO. Systemic inflammatory response syndrome. Br J Surg, 1997; 84(7)∶920.
- 2. 全竹富， 黎介寿. 腹腔感染致多器官功能衰竭的动物模型. 中华实验外科杂志， 1991； 8(2)∶91.
- 3. Eiseman B, Sloan R, Hansbrough J, et al. Multiple organ failure: clinical and experimental. Am Surg, 1980; 46(1)∶14.
- 4. Creasey AA, Stevens P, Kenney J, et al. Endotoxin and cytokine profile in plasma of baboons challenged with lethal and sublethal E. Coli. Circ Shock, 1991; 33(2)∶84.
- 5. Spinas GA, Bloesch D, Keller LL, et al. Pretreatment with ibuprofen augemnts criculating TNFα, IL6, and elastase during acute endotoxaemia. J Infect Dis, 1991; 163(1)∶89.
- 6. Fisher CJ Jr, Slotaman CJ, Opal SM, et al. Initial evaluation of human recombinant IL1ra in the treatment of sepsis syndrome. Crit Care Med, 1994; 22(1)∶12.
- 7. Cannon JG, Van der Meer JWM, Kwiatowski D, et al. IL1 in human plasma: optimization of blood collection, plasma extraction, and radioimmunoassay methods. Lymphokine Res, 1988; 7(6)∶457.
- 8. Zhong WW, Burke PA, Hand AT, et al. Regulation of cytokine mRNA expression in LPSstimulated human macrophages. Arch Surg, 1993; 128(2)∶158.
