To observe the pathologic changes of normal tissue in nude mice after peritoneal perfusion with hyperthermia, hyper-osmolar solution and mitomycin C (MMC). Fifty BALB/c nunu mices (7-10 weeks old) bearing HT-2 lines were chosen for the study, and were randomly divided into five groups: isotonic solution (control group), hyperosmolar solution (HOS group), HOS plus MMC group, hyperthermia (HT group) and HOS plus HT plus MMC group. After continuous hyperthermic peritoneal perfusion (42℃/30min) with 7.5% NaCl and 5μg/ml MMC, the liver, spleen, small intestine and kidney were examined by light microscopy. Results: ①In HOS and HOS plus MMC groups, no changes of liver, spleen and kidney were found. ②In HT and HOS plus HT plus MMC groups, slight degeneration of liver, hyperemia of spleen, swelling of kidney tubule cells and small intestine were found. ③In HOS plus HT plus MMC group, partial loss of small intestinal villi were also observed. Conclusioin: After continuous hyperthermic perfusion conbined with hyper-osmolar solutions and mitomycin C, a slight injury was showed in normal tissue of nude mice.
Citation：
Cao Hong,Wang Daike,Wu Xiaohua.. EFFECT OF PERITONEAL PERFUSION WITH HYPERTHERMIA, HYPER-OSMOLAR SOLUTIONS AND ANTINEOPLASTIC DRUG ON NORMAL TISSUE IN NUDE MICE. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1999, 6(2): 79-80. doi:
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- 1. 曹红， 王代科. 温热高渗液化疗药物对人大肠癌移植瘤的体内作用. 中华实验外科杂志， 1996； 13(5)∶121.
- 2. 曹红， 王代科， 吴小华等. 高渗热化疗对人结肠癌裸鼠移植瘤的病理影响. 第三军医大学学报， 1998； 20(2)∶69.
- 3. 史历， 刘旭， 刘曼杰等. 人结肠腺癌裸鼠皮下和腹腔移植瘤株的建立及主要生物学特性的观察. 实用肿瘤学杂志， 1993； 16(3)∶34.
- 4. Larkin JM, Edwards WS, Daniel E, et al. Systemic thermotherapy: description of a method and physiologic tolerance in clinical subjects. Cancer, 1977; 40(6)∶3155.
- 5. Zavagno G, Vespa D, Moschin N, et al. Effect of hyperthermia on isolated perfused rat liver. Eur Surg Res, 1989; 21(5)∶243.
- 6. Gilly FN, Carry PY, Sayag AC, et al. Tolerance of intraperitoneal chemohyperthermia with mitomycin C： in vivo study in dogs. Hyperthermia, 1992; 8(5)∶659.
- 7. Tsubouchi S, Kano E, Nishimoto Y, et al. The effect of microwave induced whole body hyperthermia on the mucosa of mouse small intestine. J Radiat Res, 1984; 25(2)∶131.
