EFFECT OF LOW DENSITY LIPOPROTEIN RECEPTOR ACTIVITY OF HEPATOCYTE ON BILE ACID DURING GALLSTONE FORMATION (AN EXPERIMENTAL STUDY)_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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Department of General Surgery, First Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041;

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Keywords：

Low density lipoprotein receptor of hepatocyte; Bile acid; Gallstone; Animal experiment

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To study the mechanism of cholesterol gallstone formation, rabbit models were induced by feeding with high cholesterol diet. Bile acids were tested with bi-wavelengh thin layer scan and low density lipoprotein receptor activity of hepatocytes binding to 125I-LDL were tested with radio immunoassay in different feeding phases as 1,2,3 and 4-week groups, as well as the control group. The results showed that cholesterol gallstones in 2,3 and 4-week groups were induced in respectively. The contents of glucocholic acid (GCA) in bile were decreased significantly (vs control group, Ｐ＜0.05). The Bmax values of LDL receptor of hepatocytes binding to 125I-LDL were decreased significantly (Ｐ＜0.05). Kd values of those gradually increased (Ｐ＜0.05). These suggest that the decreased activity of LDL receptor of hepatocytes would reduce the synthesis of GCA, thus resulting in the formation of cholesterol gallstones.

Citation： Zhao Jichun,Shu Ye,Hou Caifang,et al.. EFFECT OF LOW DENSITY LIPOPROTEIN RECEPTOR ACTIVITY OF HEPATOCYTE ON BILE ACID DURING GALLSTONE FORMATION (AN EXPERIMENTAL STUDY). CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1999, 6(4): 207-209. doi: Copy

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1. 赵纪春，肖路加，朱红等. 兔胆囊结石形成过程中脂代谢和载脂蛋白变化研究. 华西医科大学学报， 1997； 28(4)∶361.
2. 孟高渊，东云华，李彬等. 九种混合胆汁酸的薄层光密度定量分析. 华西预防医学， 1989； 43(1)∶24.
3. Makwell MA. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Ann Biochem, 1978; 87(2)∶206.
4. Brown MS, Goldstein JL. A receptormediated pathway for cholesterol homeostasis. Science, 1986; 232(1)∶34.
5. Seglen PO. Preparation of rat liver cells. Exp Cell Res, 1993; 82(2)∶391.
6. Kern F Jr. Effects of dietary cholesterol on cholesterol and bile acid homeostasis in patients with cholesterol gallstones. J Clin Invest, 1994; 93(3)∶1186.
7. Rudel L, Deckelman C, Wilson M, et al. Dietary cholesterol and down regulation of cholesterol 7αhydroxylase and cholesterol absorption in African green monkeys. J Clin Invest, 1994; 93(8)∶2463.
8. Khallon J, Riottot M, Parquet M, et al. Biodynamics of cholesterol and bile acids in the lithasic hamster. Br J Nutr, 1991; 66(3)∶479.
9. Hayes KC, Livingston A, Trautwein EA. Dietary impact on biliary lipids and gallstones. Ann. Rev Nutr, 1992; 12∶299.
10. Nicolosi RJ, Stucchi AF, Kowala MC, et al. Effect of dietary fat saturation and cholesterol on LDL composition and metabolism. Arteriosclerosis, 1990; 10(1)∶119.
11. Everson GT, Daggy BP, Mckinley GT, et al. Dietary fiber (psyllium) lowers LDL cholesterol by stimulating bile acid synthesis in man. Hepatology, 1991; 14(Suppl)∶148a.
12. Reihner E, Angelin B, Rudling M, et al. Regulation of hepatic cholesterol metabolism in human: stimulatory effects of cholestyramine on HMGCoA reductase activity and low density lipoprotein receptor expression in gallstone patients. J Lipid Res, 1990; 31(12)∶2219.
13. Nanjee MN, Miller NE. Human hepatic low density lipoprotein receptors : associations of receptor activities in vitro with plasma lipid and apolipoprotein concentrations in vivo. Biochem Biophys Acta, 1989; 1002(2)∶245.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

EFFECT OF LOW DENSITY LIPOPROTEIN RECEPTOR ACTIVITY OF HEPATOCYTE ON BILE ACID DURING GALLSTONE FORMATION (AN EXPERIMENTAL STUDY)

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