A PRIMARY EXPERIMENTAL STUDY OF SUPPRESSIVE EFFECTS OF NM23-H1 ON METASTASIS OF PRIMARY HEPATOCELLULAR CARCINOMA CELLS_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

Zhang Kaitai ¹ , Wang Yuzhi ¹ , Han Benli. ²

Institute of Beijing Radiation Medicine, Beijing 100850;

Corresponding author：

Keywords：

Primary hepatocellular carcinoma; Invasion; nm23-H1 gene

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For an advanced elucidation of mechanisms of nm23-H1 suppressive effects on metastasis of primary hepatocellular carcinoma (HCC), it is necessary to investigate the correlation between nm23-H1 expression and relative factors involved in the HCC invasion. In present report, full-length cDNA of nm23-H1 was subcloned into pBKCMV vector and transfected into HCC cell line to observe its effects on invasion, cytosolic free Ca2+ and Nras mRNA expression. The results showed that lower expression of N-ras and higher cytosolic free Ca2+ in transfected cell line were detected, while the potential of invasion was depressed. It suggests that the suppressive effects on HCC metastasis might interact with intracellular signal transduction which is essential for stimulating cell invasion.

Citation： Zhang Kaitai,Wang Yuzhi,Han Benli.. A PRIMARY EXPERIMENTAL STUDY OF SUPPRESSIVE EFFECTS OF NM23-H1 ON METASTASIS OF PRIMARY HEPATOCELLULAR CARCINOMA CELLS. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1999, 6(5): 273-275. doi: Copy

1.	Seishima M, Esaki C, Osada K. Pemphigus IgG, but not bullous pemphigoid IgG, causes a trasient increase in intracellular calcium and inositol 1,4,5triphosphate in DJM1 cells, a squamous cell carcinoma line. J Invest Dermatol, 1995; 104(1)∶33.
2.	Munoz Dorado J, Almaula N, Inouye S. Autophosphorylation of nucleoside diphosphate kinase from myxococcus xanthus. J Bact, 1993; 175(4)∶1176.
3.	MacKonald NJ, De La Rosa A, Benedict M. A novel phosphorylation of nm23, and not its nucleoside diphophate kinase actinity, correlates with suppression of tumor metastasis. J Biol Chem, 1993; 269(13)∶25780.
4.	Fuller LC, Allen MH, Montesu M. Expression of Ecadherin in human epidermal nonmelanoma cutaneous tumors. Br J Dermatol, 1996; 134(1)∶28.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

A PRIMARY EXPERIMENTAL STUDY OF SUPPRESSIVE EFFECTS OF NM23-H1 ON METASTASIS OF PRIMARY HEPATOCELLULAR CARCINOMA CELLS

Abstract Full text Figures/Tables Video References Cited by

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