On the basis of established JF305 cell line from human pancreatic cancer at this university, cell clone technique, cell electrophoresis, flower cytometer, and cancer orthotopically implanted nude mice technique were used to establish the sublines with different metastatic potential from human pancreatic cancer line-JF305 and the nude mice model implanted orthotopically with human pancreatic cancer monoclonal sublines with different metastatic potential. The results showed that the monoclonal cell sublines with different metastatic potential from human pancreatic caner-JF305 and the nude mice model implanted orthotopically with the sublines, would provided a useful method to study the metastatic mechanism of human pancreatic cancer.
Citation:
Cui Hong,He Sanguang,Liu Yongfeng,et al.. THE ESTABLISHMENT OF THE MONOCLONAL CELL SUBLINES WITH DIFFERENT METASTATIC POTENTIAL FROM HUMAN PANCREATIC CANCER JF305. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1999, 6(6): 334-335. doi:
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1. |
李昕, 王宏, 姜奕等. 表达p53基因的人胰腺癌细胞系JF305的建立. 中华肿瘤杂志, 1994; 16(3)∶181.
|
2. |
高进主编. 癌的侵袭与转移. 第1版. 北京: 北京医科大学中国协和医科大学联合出版社, 1996∶2~3.
|
3. |
高进, 钱书森, 薛克勋等. 两个小鼠前胃癌细胞株的不同电泳率与转移率之间的关系观察. 中国病理学杂志, 1988; 17(1)∶45.
|
4. |
万海粟, 赵雪梅, 高进. 小鼠肺腺癌LA795细胞系不同转移克隆株的分离及其膜质流动性的研究. 中国实验动物学杂志, 1993; 3(2)∶102.
|
5. |
刘亚琴, 高进. 转移潜能不同的小鼠肺腺癌亚系的生物学性质的比较研究. 基础医学与临床, 1993; 13(2)∶36.
|
6. |
Fidler JJ. Critical factors in the biology of human cancer metastasis. Cancer Res, 1990; 50(19)∶6130.
|
7. |
刘秋珍, 脱朝伟, 张龙石等. 两株人胰腺癌裸鼠胰腺内原位移植模型的建立及其生物学特性. 解放军医学杂志, 1993; 18(2)∶124.
|
- 1. 李昕, 王宏, 姜奕等. 表达p53基因的人胰腺癌细胞系JF305的建立. 中华肿瘤杂志, 1994; 16(3)∶181.
- 2. 高进主编. 癌的侵袭与转移. 第1版. 北京: 北京医科大学中国协和医科大学联合出版社, 1996∶2~3.
- 3. 高进, 钱书森, 薛克勋等. 两个小鼠前胃癌细胞株的不同电泳率与转移率之间的关系观察. 中国病理学杂志, 1988; 17(1)∶45.
- 4. 万海粟, 赵雪梅, 高进. 小鼠肺腺癌LA795细胞系不同转移克隆株的分离及其膜质流动性的研究. 中国实验动物学杂志, 1993; 3(2)∶102.
- 5. 刘亚琴, 高进. 转移潜能不同的小鼠肺腺癌亚系的生物学性质的比较研究. 基础医学与临床, 1993; 13(2)∶36.
- 6. Fidler JJ. Critical factors in the biology of human cancer metastasis. Cancer Res, 1990; 50(19)∶6130.
- 7. 刘秋珍, 脱朝伟, 张龙石等. 两株人胰腺癌裸鼠胰腺内原位移植模型的建立及其生物学特性. 解放军医学杂志, 1993; 18(2)∶124.