• First Department of General Surgery, First Clinical College, China Medical University, Shenyang 110001;
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On the basis of established JF305 cell line from human pancreatic cancer at this university, cell clone technique, cell electrophoresis, flower cytometer, and cancer orthotopically implanted nude mice technique were used to establish the sublines with different metastatic potential from human pancreatic cancer line-JF305 and the nude mice model implanted orthotopically with human pancreatic cancer monoclonal sublines with different metastatic potential. The results showed that the monoclonal cell sublines with different metastatic potential from human pancreatic caner-JF305 and the nude mice model implanted orthotopically with the sublines, would provided a useful method to study the metastatic mechanism of human pancreatic cancer.

Citation: Cui Hong,He Sanguang,Liu Yongfeng,et al.. THE ESTABLISHMENT OF THE MONOCLONAL CELL SUBLINES WITH DIFFERENT METASTATIC POTENTIAL FROM HUMAN PANCREATIC CANCER JF305. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1999, 6(6): 334-335. doi: Copy