For research the relationship between the expression of vascular endothelial growth factor (VEGF) and the cell proliferation. The expression of VEGF was evaluated while the cell cycle of hepatoma cell line Hep G2, which was synchronized at G0 phase with serum deprivation, and reinitiated with TPA and blocked with antisense oligonucleotides of c-jun. Results: Hep G2 cell did not express VEGF at G0 phase. TPA could induce the expression of VEGF as well as initiation of cell cycle. The antisense oligonucleotides of c-jun could prohibit the expression of VEGF and arrest the cell cycle at G0 phase. Conclusion: The fact that the expression of VEGF accompanies the initiation of cell cycle suggests that they be regulated by the same singnal pathway, the expression of VEGF may be a marker indicating the proliferation of hepatoma cells.
Citation：
Zhang Xiaoshi,Yao Zhenxiang,Tao Xiaohong.. THE RELATIONSHIP BETWEEN THE EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR AND INITIATION OF CELL CYCLE IN HEPATOMA CELL LINE HEPG2. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1999, 6(6): 342-343. doi:
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Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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- 1. Mattern J, Koomagi R, Volm M. Association of vascular endothelial growth factor expression with intratumoral microvessel density and tumor cell proliferation in human epidermoid lung carcinomas. Br J Cancer, 1996; 73(7)∶931.
- 2. Chow NH, Hsu PI, Lin XZ, et al. Expression of vascular endothelial growth factor in normal and hepatocellular carcinomas: an immunohisto chemical study. Hum pathol, 1997; 28(6)∶698.
- 3. Arts J, Grimbergen J, Bosma PJ, et al. Role or cjun and proximal phorbol12myristate13acetate(PMA)responsive elements in the regulation of basal and PMAstimulated plasminogenactivator inhibitor1 gene expression on Hep G2, Eur J Biochem, 1996; 241(2)∶393.
- 4. Tischer E, Mitchell R, Hartman T, et al. The human gene for vascular endothelial growth factor. J Biol Chen, 1991; 266(18)∶11947.
- 5. Kolch W, MartinyBaron G, Kieser A, et al. Regulation of the expression of the VEGF/VPF and its receptors: role in tumor angiogenesis. Breast Cancer Res Treat, 1995; 36(2)∶139.
- 6. Karin M. The regulation of AP1 activity by mitogenactivated protein kinase. J Biol Chem, 1995; 270(28)∶16483.
- 7. Yoshida S, Ono M, Shono T, et al. Involvement of interleukin8, vascular endothelial growth factor, and basic fibroblast factor in tumor necrosis factor alphadependent angiogenesis. Mol Cell Biol, 1997; 17(7)∶4015.
- 8. Brusselbach S, Mohlesteinlein U, Wang ZQ, et al. Cell proliferation and cell cycle progression are not impaired in fibroblasts and ES cells laking cfos, Oncogene, 1995; 10(1)∶79.
