Human SW480 colonic cancer cell line was evaluated for its growth response to pentagastrin, gastrin receptor antagonist proglumide (PGL) in vitro by MTT assay and flow cytometry. The results showed that gastrin possessed a proliferative effect on SW480 cell, PGL alone had no obvious effect on SW480 cell, but it inhibited gastrin-induced growth of SW480 cell with dosage dependent when it was used with gastrin, its inhibitive effect did not steadly increase at a dose＞32μg/ml. This suggests that effect of gastrin is achieved via gastrin receptor. Gastrin promoted the sythesis of DNA, protein and triggered the cancer cell shifting from phase G0/G1 to phase S, G2M. PLG inhibited the effect of gastrin, it suggests that gastrin possessed a proliferation on SW480 cell at post receptor is achieved by the effect of gastrin on cell cycle.
Citation：
Xie Shangkui,He Shuangwu,Wu Yingai,et al.. EFFECTS OF GASTRIN AND GASTRIN RECEPTOR ANTAGONIST ON HUMAN SW480 COLONIC CANCER CELL LINE IN VITRO. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1998, 5(1): 1-3. doi:
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Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
| 1. |
Johnson LR. New aspects of the trophic actions of gastrointestinal hormones. Gastroenterology, 1977; 72(3)∶788.
|
| 2. |
Watson S, Durrant LG, Morris D. Gastrin:growth enhancing effects on human gastric and colonic tumor cells. Br J Cancer, 1989; 59(3)∶554.
|
| 3. |
Mosman T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 1983; 63(1)∶55.
|
| 4. |
宋平根，李素文.流式细胞术的原理和应用.第1版.北京:北京师范大学出版社， 1992∶254～268.
|
| 5. |
Kusyk CJ, Mcniel NO, Johnson LR, et al. Stimulation of growth of a colon cancer cell line by gastrin. Am J Physiol, 1986; 251(7)∶G597.
|
| 6. |
Imdah LA, Eggstein S, Crone C, et al. Growth of colorectal carcinoma cells: regulation in vitro by gastrin, pentagastrin and the gastrinreceptor antagonist proglumide. J Cancer Clin Oncol, 1989; 115(4)∶388.
|
| 7. |
Smith JP, Solomon TE. Effects of gastrin, proglumide, and somatostatin on growth of human colon cancer. Gastroenterology,1988; 95(1)∶1541.
|
| 8. |
CivitellS, GalganiP, PacchiarottiMC, et al. Blood gastrin and colorectal neoplasma. GChir, 1994; 15(5)∶219.
|
| 9. |
De-Bruine AP, Wiggers T, Beek C, et al. Endocrine cells in colorectal adenocarcinomas: in cidence,hormone profile and prognostic relevance. In J Cancer, 1993; 54(5)∶765.
|
- 1. Johnson LR. New aspects of the trophic actions of gastrointestinal hormones. Gastroenterology, 1977; 72(3)∶788.
- 2. Watson S, Durrant LG, Morris D. Gastrin:growth enhancing effects on human gastric and colonic tumor cells. Br J Cancer, 1989; 59(3)∶554.
- 3. Mosman T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 1983; 63(1)∶55.
- 4. 宋平根，李素文.流式细胞术的原理和应用.第1版.北京:北京师范大学出版社， 1992∶254～268.
- 5. Kusyk CJ, Mcniel NO, Johnson LR, et al. Stimulation of growth of a colon cancer cell line by gastrin. Am J Physiol, 1986; 251(7)∶G597.
- 6. Imdah LA, Eggstein S, Crone C, et al. Growth of colorectal carcinoma cells: regulation in vitro by gastrin, pentagastrin and the gastrinreceptor antagonist proglumide. J Cancer Clin Oncol, 1989; 115(4)∶388.
- 7. Smith JP, Solomon TE. Effects of gastrin, proglumide, and somatostatin on growth of human colon cancer. Gastroenterology,1988; 95(1)∶1541.
- 8. CivitellS, GalganiP, PacchiarottiMC, et al. Blood gastrin and colorectal neoplasma. GChir, 1994; 15(5)∶219.
- 9. De-Bruine AP, Wiggers T, Beek C, et al. Endocrine cells in colorectal adenocarcinomas: in cidence,hormone profile and prognostic relevance. In J Cancer, 1993; 54(5)∶765.
