Human SW480 colonic cancer cell line was evaluated for its growth response to pentagastrin, gastrin receptor antagonist proglumide (PGL) in vitro by MTT assay and flow cytometry. The results showed that gastrin possessed a proliferative effect on SW480 cell, PGL alone had no obvious effect on SW480 cell, but it inhibited gastrin-induced growth of SW480 cell with dosage dependent when it was used with gastrin, its inhibitive effect did not steadly increase at a dose＞32μg/ml. This suggests that effect of gastrin is achieved via gastrin receptor. Gastrin promoted the sythesis of DNA, protein and triggered the cancer cell shifting from phase G0/G1 to phase S, G2M. PLG inhibited the effect of gastrin, it suggests that gastrin possessed a proliferation on SW480 cell at post receptor is achieved by the effect of gastrin on cell cycle.
Citation： Xie Shangkui,He Shuangwu,Wu Yingai,et al.. EFFECTS OF GASTRIN AND GASTRIN RECEPTOR ANTAGONIST ON HUMAN SW480 COLONIC CANCER CELL LINE IN VITRO. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1998, 5(1): 1-3. doi: Copy