A STUDY ON BACTERIAL DNA IN CHOLESTERAL GALLSTONES AND ITS CLINICAL SIGNIFICANCE BY NP-PCR_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

Wu Xiaoting ¹ , Xiao Lujia ² , Li Jieshou ¹ , et al. ³

Research Institute of General Surgery, Nanjing General Hospital of PLA, Nanjing 210002;

Corresponding author：

Keywords：

Cholesterol gallstone; Bacteria; Deoxyribonucleic acid; 16S rRNA; Polymerase chain reaction

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The aim of the this study was to search for bacterial DNA sequences in cholesterol gallstones with negative bacterial culture by NP-PCR technique. Bacterial gene fragments were amplified in vitro from DNA which were extracted from cholesterol gallstones in gallbladder for identifying the existence of bacteria. The gallbladder gallstones of 30 patients were analysed. Bacterial DNA was found in the stones of 26 patients, indicating that most cholesterol gallstones harbor bacterial DNA.

Citation： Wu Xiaoting,Xiao Lujia,Li Jieshou,et al.. A STUDY ON BACTERIAL DNA IN CHOLESTERAL GALLSTONES AND ITS CLINICAL SIGNIFICANCE BY NP-PCR. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1998, 5(2): 67-69. doi: Copy

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1. Holzbach RT, Busch N. Nucleation and growth of cholesterol crystals. Gastroenterol Clin North Am, 1991; 20(1)∶67.
2. Swidsinski A, Ludwig N, Pahlig H, et al. Molecular genetic evidence of bacterial colonization of cholesterol gallstones. Gastroenterology, 1995; 108(3)∶860.
3. 林万明主编. PCR技术操作和应用指南. 北京：人民军医出版社， 1995∶231～232.
4. Goodhart GL, Levison ME, Trotman BW, et al. Pigment vs cholesterol lithiasis. Bacteriology of gallbladder stone, bile and tissue correlated with biliary lipid analysis. Am J Dig Dis, 1978; 23(10)∶877.
5. Aurelius E, Johansson B, Skoldenberg B, et al. Rapid diagnosis of herpes simplex encephalitis by nested polymerase chain reaction assay of cerebrospinal fluid. Lancet, 1991; 337(8735)∶189.
6. Schleifer KH, Ludwig W. Phylogenetic relationships among bacteria. In： Fernholm B, Bremer K, Jornval H, eds. The hierarchy of life. Amsterdam: Elsevier, 1989∶ 278～282.
7. Sung JY, Costerton JW, Shaffer EA. Defense system in the biliary tract against bacterial infection. Dig Dis Sci, 1992; 37(5)∶689.
8. 伍晓汀，肖路加. 细菌在胆石形成中的作用研究进展. 国外医学外科学分册， 1996； 23(5)∶277.
9. Neiderhiser DH, Roth HP, Webster LT. Studies on the importance of lecithin for cholesterol solubilization in bile. Gastroenterology, 1990; 99(1)∶1798.
10. Soloway RD, Growther RS. Bacteria and cholesterol gallstones: molecular biology comes to gallstones pathogenesis. Gastroenterology, 1995; 108(3)∶934.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

A STUDY ON BACTERIAL DNA IN CHOLESTERAL GALLSTONES AND ITS CLINICAL SIGNIFICANCE BY NP-PCR

Abstract Full text Figures/Tables Video References Cited by

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