The effects of pentagastrin (PG) on the viable cell count (Α value) and the synthesis of DNA (CPM value) of primary cultured large bowel carcinoma cells in 25 patients were evaluated in vitro by MTT assay,3H-TdR incorporation. The results showed that Α value and CPM value in well， moderately and poorly-differentiated carcinoma cells were higher than normal control (Ｐ lt;0.01，Ｐ＜0.05). The proliferative effect was significant at a dose of 0.3907 μg/ml in well-differentiated carcinoma cells, and at a dose of 6.2500μg/ml in moderately and poorly-differentiated carcinoma cells. These indicat that PG has the proliferative effect on large bowel carcinoma cells. These results provide an experimental foundation for the endocrine therapy for patients with large intestine carcinoma, especially by using gastrin receptor antagonists for well-differentiated carcinoma.
Citation：
He Shuangwu,Xie Shangkui,Zhang Lianyang.. EFFECTS OF GASTRIN ON PROLIFERATION OF PRIMARY CULTURED CANCER CELLS OF HUMAN LARGE INTESTINE. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1998, 5(3): 143-145. doi:
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- 2. Kiss R, Salman I, Pauwels O, et al. In vitro influence of gastrin, oestradiol and gonadotropinreleasing hormone on HCT15 and LOVO human colorectal neoplastic cell proliferation. Eur J Cancer, 1991; 27(10)∶1268.
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- 5. Mauss S, Niederau C, Hengels K. Effects of gastrin, proglumide, loxiglumide and L365.260 on growth of human colon carcinoma cells .Anticancer Res, 1994; 14(1A)∶215.
