To quantitatively measure the α1-adrenergic receptors and select the suitable conditions of receptor-ligand binding assay. Radioligand binding analysis was used to measure the concentration of α1-adrenergic receptors, and the measuring conditions were selected, respectively. Under the selected conditions, the α1-adrenergic receptors in lier plasma membranes of 8 nonhepatopathy subjects were measured.Under such suitable conditions as ３H-prazosin concentration 0.1-2.0nmol/L, incubating temperature 37℃, reaction time 20 minutes and seperating free ligand from system with double layer of filter paper, the binding of 3H-prazosin to α1receptors from 8 nonhepatopathy subjects were saturable with a high affinity. The Bmax, Kd, RMC and Hill coefficient were 142.1±14.1 fmol per milligram of protein, 0.2382±0.0548 nM, 739.0±167.6 fmol per gram of liver and 0.90±0.03, respectively.The suitable conditions may be very important for measuring α1-adrenergic receptors in human liver plasma membranes, which should be considered in hepatopathy studies.
Citation：
Zhang Youcheng,Leng Xisheng,Peng Jirun.. MEASURING CONDITIONS OF HUMAN HEPATIC α1-ADRENERGIC RECEPTORS WITH RADIOLIGAND BINDING ANALYSIS. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1998, 5(4): 202-204. doi:
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- 1. Rosen SG, Clutter WE, Shah SD, et al. Direct αadrenergic stimulation of hepatic glucose production in human subjects. Am J Physiol, 1983; 245(5)∶E616.
- 2. 冷希圣，黄萃庭，杜如昱.组织胺、去甲肾上腺素、多巴胺、胰高血糖素对肝脏血流动力学的影响.北京医学院学报， 1982； 14(1)∶1.
- 3. Michalopoulos G, Cianciulli HD, Novotny AR, et al. Liver regeneration studies with rat hepatocytes in primary culture. Cancer Res, 1982; 42(8)∶4673.
- 4. Williams LT, Mullikin D, Lefkowitz RJ. Identification of αadrenergic receptors in uterine smooth muscle membranes by 3Hdihydroergocryptine binding. J Biol Chem, 1976; 251(22)∶6915.
- 5. Bradford MM. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem, 1976; 72(2)∶248.
- 6. 夏宗勤主编.实验核医学与核药学.第1版.上海：同济大学出版社， 1989∶220～240.
- 7. 冯国平.受体分析常用数据处理方法的比较.中华核医学杂志，1987; 7(3)∶167.
- 8. Kawai Y, Powell A, Arinze IJ. Adrenergic receptors in human liver plasma membranes: predominance of beta 2and alpha 1receptor subtypes. J Clin Endocrinol Metab, 1986; 62(5)∶827.
- 9. Hwang TL, Lau YT, Huang SF,et al. Changes of α1adrenergic receptors in human liver during intraabdominal sepsis. Hepatology, 1994; 20(3)∶638.
