To investigate the characteristic of antibodies that induce the rejection of xenografting,SD rats were immunized with NIH mice spleen to prepare rat antimouse antiseras. The antibodies were purified by caprylic acid precipitation and hydroxy lapatite(HPT)chromatography.The results showed that the title of rat antimouse antisera was 1∶640,and the antibodies purified by caprylic acidHPT had reliable purity and good immunocompetence.This method coule be used in preparation and research of major histocompatibility antibodies.
Citation:
兰平,王吉甫. PREPARATION OF RAT ANTIBODIES AGAINST MOUSE MAJOR HISTOCOMPATIBILITY ANTIGEN. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1997, 4(3): 129-131. doi:
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金玫薷,张瑞忠,鲍世民.小鼠H2,Hc和Thy1单价特异性抗血清的制作研究.免疫学杂志,1991;7(4)∶220.
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Ogden JR,Leung K.Purification of murine monoclonal antibodies by caprylic acid.J Immunol Methods,1988;111∶283.
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Stanker LH,Vanderlan M.Onestep purification of mouse monoclonal antibodies from ascites fluid by hydroxy lapatite chromatography. J Immunol Methods,1985;76∶157.
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牛占坡,来锦明,罗学姬.腹水中单克隆抗体的简单快速纯化法.中国医学科学院学报,1987;9(3)∶212.
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- 1. 金玫薷,张瑞忠,鲍世民.小鼠H2,Hc和Thy1单价特异性抗血清的制作研究.免疫学杂志,1991;7(4)∶220.
- 2. Ogden JR,Leung K.Purification of murine monoclonal antibodies by caprylic acid.J Immunol Methods,1988;111∶283.
- 3. Stanker LH,Vanderlan M.Onestep purification of mouse monoclonal antibodies from ascites fluid by hydroxy lapatite chromatography. J Immunol Methods,1985;76∶157.
- 4. 牛占坡,来锦明,罗学姬.腹水中单克隆抗体的简单快速纯化法.中国医学科学院学报,1987;9(3)∶212.