Objective To investigate the effect of aerosolized perfluorocarbon (PFC) (FC77) on gas exchange，histopathological changes of lung in acute lung injury and pulmonary expression of tumor necrosis factor-α (TNF-α) mRNA.Methods After acute lung injury (ALI) was induced by oleic acid (OA)，16 rabbits were assigned randomly into 2 groups,ie.aerosolized perfluorocarbon group (PFC group) and conventional mechanical ventilation group (CMV group).Gas exchange parameters were measured before and after ALI，at 1，2，3，4 h after treatment.Histological sections taken from 6 different parts of lung were stained by hematoxylin and eosion.The express of TNF-α mRNA in the 2 different parts of lung were detected by in situ hybridization (ISH).Results Compared with CMV group，the PaO2 and static lung compliance (CLst) were significantly increased (P lt;0.05)，the histopathological lesions of lung were attenuated，and the TNF-α mRNA expression was decreased significantly in PFC group (all P lt;0.05).There was more expression of TNF-α mRNA in backside than that in foreside of lung in two groups (P lt;0.05).Conclusion Aerosolized perfluorocarbon (PFC) can decrease expression of tumor necrosis factor-α mRNA in the lung，and improve the CLst and oxygenation during acute lung injury.
Citation：
HUANG Zhijian,LI Qi,WANG Changzheng,QIAN Guisheng,SONG Peng. Effect of aerosolized perfluorocarbon on the tumor necrosis factor-α mRNA expression and lung histopathology in rabbits with acute lung injury. Chinese Journal of Respiratory and Critical Care Medicine, 2007, 6(1): 45-48. doi:
Copy
Copyright © the editorial department of Chinese Journal of Respiratory and Critical Care Medicine of West China Medical Publisher. All rights reserved
| 1. |
Leach CL，Greenspan JS，Rubenstein SD，et al.Partial liquid ventilation with perflubron in premature infants with severe respiratory distress syndrome.N Engl J Med，1996，335：761-767.
|
| 2. |
Ragaller M，Bleyl J，Tscho U，et al.Effects of inhalation of perfluorocarbon aerosol on oxygenation and pulmonary function compared to PGI2 inhalation in a sheep model of oleic acid-induced lung injury.Intensive Care Med，2001，27：889-897.
|
| 3. |
Bleyl JU，Ragaller M，Tscho U，et al.Vaporized perfluorocarbon improves oxygenation and pulmonary function in an ovine model of acute respiratory distress syndrome.Anesthesiology，1999，91：461-469.
|
| 4. |
Kelly KP，Stenson BJ，Drummond GB.Randomised comparison of partial liquid ventilation，nebulised perfluorocarbon，porcine surfactant，artificial surfactant，and combined treatments on oxygenation，lung mechanics，and survival in rabbits after saline lung lavage.Intensive Care Med，2000，26：1523-1530.
|
| 5. |
Kandler MA，von der Hardt K，Schoof E，et al.Persistent improvement of gas exchange and lung mechanics by aerosolized perfluorocarbon.Am J Respir Crit Care Med，2001，164：31-35.
|
| 6. |
王晓光，刘又宁.全氟化碳治疗急性肺损伤/急性呼吸窘迫综合征的新进展.国外医学呼吸系统分册，2005，25：517-519.
|
| 7. |
Nakazawa K，Yokoyama K，Matsuzawa Y，et al.Pulmonary administration of prostacyclin (PGI2) during partial liquid ventilation in an oleic acid-induced lung injury：inhalation of aerosol or intratracheal instillation? Intensive Care Med，2001，27：243-250.
|
| 8. |
Noble WH，Severinghaus JW.Thermal and conductivity dilution curves for rapid quantitation of pulmonary edema.J Appl Physiol，1972，32：770-775.
|
| 9. |
钱桂生.在实践中提高对急性肺损伤和急性呼吸窘迫综合征新概念的认识.中国呼吸与危重监护杂志，2002，1：199-201.
|
| 10. |
von der Hardt K，Kandler MA，Fink L，et al.Laser-assisted microdissection and real-time PCR detect anti-inflammatory effect of perfluorocarbon.Am J Physiol Lung Cell Mol Physiol，2003，285：L55-L62.
|
| 11. |
Quintel M，Heine M，Hirschl RB，et al.Effects of partial liquid ventilation on lung injury in a model of acute respiratory failure：a histologic and morphometric analysis.Crit Care Med，1998，26：833-843.
|
| 12. |
朱光发，张国清，郭海英，等.肺表面活性物质对急性呼吸窘迫综合征肺内炎症反应的影响.中国呼吸与危重监护杂志，2002，1：206-209.
|
| 13. |
樊毫军，张健鹏，刘又宁.吸入全氟化碳治疗急性肺损伤研究进展.中国呼吸与危重监护杂志，2006，5：63-65.
|
| 14. |
Brower RG，Ware LB，Berthiaume Y，et al.Treatment of ARDS.Chest，2001，120：1347-1367.
|
| 15. |
彭雪梅，孙婵，王华东.部分液体通气对急性肺损伤小猪肺组织结构及气体交换的影响.中国病理生理杂志，2005，21：129-133.
|
- 1. Leach CL，Greenspan JS，Rubenstein SD，et al.Partial liquid ventilation with perflubron in premature infants with severe respiratory distress syndrome.N Engl J Med，1996，335：761-767.
- 2. Ragaller M，Bleyl J，Tscho U，et al.Effects of inhalation of perfluorocarbon aerosol on oxygenation and pulmonary function compared to PGI2 inhalation in a sheep model of oleic acid-induced lung injury.Intensive Care Med，2001，27：889-897.
- 3. Bleyl JU，Ragaller M，Tscho U，et al.Vaporized perfluorocarbon improves oxygenation and pulmonary function in an ovine model of acute respiratory distress syndrome.Anesthesiology，1999，91：461-469.
- 4. Kelly KP，Stenson BJ，Drummond GB.Randomised comparison of partial liquid ventilation，nebulised perfluorocarbon，porcine surfactant，artificial surfactant，and combined treatments on oxygenation，lung mechanics，and survival in rabbits after saline lung lavage.Intensive Care Med，2000，26：1523-1530.
- 5. Kandler MA，von der Hardt K，Schoof E，et al.Persistent improvement of gas exchange and lung mechanics by aerosolized perfluorocarbon.Am J Respir Crit Care Med，2001，164：31-35.
- 6. 王晓光，刘又宁.全氟化碳治疗急性肺损伤/急性呼吸窘迫综合征的新进展.国外医学呼吸系统分册，2005，25：517-519.
- 7. Nakazawa K，Yokoyama K，Matsuzawa Y，et al.Pulmonary administration of prostacyclin (PGI2) during partial liquid ventilation in an oleic acid-induced lung injury：inhalation of aerosol or intratracheal instillation? Intensive Care Med，2001，27：243-250.
- 8. Noble WH，Severinghaus JW.Thermal and conductivity dilution curves for rapid quantitation of pulmonary edema.J Appl Physiol，1972，32：770-775.
- 9. 钱桂生.在实践中提高对急性肺损伤和急性呼吸窘迫综合征新概念的认识.中国呼吸与危重监护杂志，2002，1：199-201.
- 10. von der Hardt K，Kandler MA，Fink L，et al.Laser-assisted microdissection and real-time PCR detect anti-inflammatory effect of perfluorocarbon.Am J Physiol Lung Cell Mol Physiol，2003，285：L55-L62.
- 11. Quintel M，Heine M，Hirschl RB，et al.Effects of partial liquid ventilation on lung injury in a model of acute respiratory failure：a histologic and morphometric analysis.Crit Care Med，1998，26：833-843.
- 12. 朱光发，张国清，郭海英，等.肺表面活性物质对急性呼吸窘迫综合征肺内炎症反应的影响.中国呼吸与危重监护杂志，2002，1：206-209.
- 13. 樊毫军，张健鹏，刘又宁.吸入全氟化碳治疗急性肺损伤研究进展.中国呼吸与危重监护杂志，2006，5：63-65.
- 14. Brower RG，Ware LB，Berthiaume Y，et al.Treatment of ARDS.Chest，2001，120：1347-1367.
- 15. 彭雪梅，孙婵，王华东.部分液体通气对急性肺损伤小猪肺组织结构及气体交换的影响.中国病理生理杂志，2005，21：129-133.
