Effect of aerosolized perfluorocarbon on the tumor necrosis factor-α mRNA expression and lung histopathology in rabbits with acute lung injury_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

HUANG Zhijian ,  LI Qi , WANG Changzheng , QIAN Guisheng , SONG Peng

.Institute of Respiratory Diseases of PLA，Xinqiao Hospital，The Third Military Medical University.Chongqing，400037，ChinaCorresponding author：LI Qi，E-mail：liqioliver@sina.com;

Corresponding author：

LI Qi, Email: liqioliver@sina.com

Keywords：

Acute lung injury; Perfluorocarbon; Pathology; Tumor necrosis factor-alpha

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Objective To investigate the effect of aerosolized perfluorocarbon (PFC) (FC77) on gas exchange，histopathological changes of lung in acute lung injury and pulmonary expression of tumor necrosis factor-α (TNF-α) mRNA.Methods After acute lung injury (ALI) was induced by oleic acid (OA)，16 rabbits were assigned randomly into 2 groups,ie.aerosolized perfluorocarbon group (PFC group) and conventional mechanical ventilation group (CMV group).Gas exchange parameters were measured before and after ALI，at 1，2，3，4 h after treatment.Histological sections taken from 6 different parts of lung were stained by hematoxylin and eosion.The express of TNF-α mRNA in the 2 different parts of lung were detected by in situ hybridization (ISH).Results Compared with CMV group，the PaO2 and static lung compliance (CLst) were significantly increased (P lt;0.05)，the histopathological lesions of lung were attenuated，and the TNF-α mRNA expression was decreased significantly in PFC group (all P lt;0.05).There was more expression of TNF-α mRNA in backside than that in foreside of lung in two groups (P lt;0.05).Conclusion Aerosolized perfluorocarbon (PFC) can decrease expression of tumor necrosis factor-α mRNA in the lung，and improve the CLst and oxygenation during acute lung injury.

Citation： HUANG Zhijian,LI Qi,WANG Changzheng,QIAN Guisheng,SONG Peng. Effect of aerosolized perfluorocarbon on the tumor necrosis factor-α mRNA expression and lung histopathology in rabbits with acute lung injury. Chinese Journal of Respiratory and Critical Care Medicine, 2007, 6(1): 45-48. doi: Copy

1.	Leach CL，Greenspan JS，Rubenstein SD，et al.Partial liquid ventilation with perflubron in premature infants with severe respiratory distress syndrome.N Engl J Med，1996，335：761-767.
2.	Ragaller M，Bleyl J，Tscho U，et al.Effects of inhalation of perfluorocarbon aerosol on oxygenation and pulmonary function compared to PGI2 inhalation in a sheep model of oleic acid-induced lung injury.Intensive Care Med，2001，27：889-897.
3.	Bleyl JU，Ragaller M，Tscho U，et al.Vaporized perfluorocarbon improves oxygenation and pulmonary function in an ovine model of acute respiratory distress syndrome.Anesthesiology，1999，91：461-469.
4.	Kelly KP，Stenson BJ，Drummond GB.Randomised comparison of partial liquid ventilation，nebulised perfluorocarbon，porcine surfactant，artificial surfactant，and combined treatments on oxygenation，lung mechanics，and survival in rabbits after saline lung lavage.Intensive Care Med，2000，26：1523-1530.
5.	Kandler MA，von der Hardt K，Schoof E，et al.Persistent improvement of gas exchange and lung mechanics by aerosolized perfluorocarbon.Am J Respir Crit Care Med，2001，164：31-35.
6.	王晓光，刘又宁.全氟化碳治疗急性肺损伤/急性呼吸窘迫综合征的新进展.国外医学呼吸系统分册，2005，25：517-519.
7.	Nakazawa K，Yokoyama K，Matsuzawa Y，et al.Pulmonary administration of prostacyclin (PGI2) during partial liquid ventilation in an oleic acid-induced lung injury：inhalation of aerosol or intratracheal instillation? Intensive Care Med，2001，27：243-250.
8.	Noble WH，Severinghaus JW.Thermal and conductivity dilution curves for rapid quantitation of pulmonary edema.J Appl Physiol，1972，32：770-775.
9.	钱桂生.在实践中提高对急性肺损伤和急性呼吸窘迫综合征新概念的认识.中国呼吸与危重监护杂志，2002，1：199-201.
10.	von der Hardt K，Kandler MA，Fink L，et al.Laser-assisted microdissection and real-time PCR detect anti-inflammatory effect of perfluorocarbon.Am J Physiol Lung Cell Mol Physiol，2003，285：L55-L62.
11.	Quintel M，Heine M，Hirschl RB，et al.Effects of partial liquid ventilation on lung injury in a model of acute respiratory failure：a histologic and morphometric analysis.Crit Care Med，1998，26：833-843.
12.	朱光发，张国清，郭海英，等.肺表面活性物质对急性呼吸窘迫综合征肺内炎症反应的影响.中国呼吸与危重监护杂志，2002，1：206-209.
13.	樊毫军，张健鹏，刘又宁.吸入全氟化碳治疗急性肺损伤研究进展.中国呼吸与危重监护杂志，2006，5：63-65.
14.	Brower RG，Ware LB，Berthiaume Y，et al.Treatment of ARDS.Chest，2001，120：1347-1367.
15.	彭雪梅，孙婵，王华东.部分液体通气对急性肺损伤小猪肺组织结构及气体交换的影响.中国病理生理杂志，2005，21：129-133.

1. Leach CL，Greenspan JS，Rubenstein SD，et al.Partial liquid ventilation with perflubron in premature infants with severe respiratory distress syndrome.N Engl J Med，1996，335：761-767.
2. Ragaller M，Bleyl J，Tscho U，et al.Effects of inhalation of perfluorocarbon aerosol on oxygenation and pulmonary function compared to PGI2 inhalation in a sheep model of oleic acid-induced lung injury.Intensive Care Med，2001，27：889-897.
3. Bleyl JU，Ragaller M，Tscho U，et al.Vaporized perfluorocarbon improves oxygenation and pulmonary function in an ovine model of acute respiratory distress syndrome.Anesthesiology，1999，91：461-469.
4. Kelly KP，Stenson BJ，Drummond GB.Randomised comparison of partial liquid ventilation，nebulised perfluorocarbon，porcine surfactant，artificial surfactant，and combined treatments on oxygenation，lung mechanics，and survival in rabbits after saline lung lavage.Intensive Care Med，2000，26：1523-1530.
5. Kandler MA，von der Hardt K，Schoof E，et al.Persistent improvement of gas exchange and lung mechanics by aerosolized perfluorocarbon.Am J Respir Crit Care Med，2001，164：31-35.
6. 王晓光，刘又宁.全氟化碳治疗急性肺损伤/急性呼吸窘迫综合征的新进展.国外医学呼吸系统分册，2005，25：517-519.
7. Nakazawa K，Yokoyama K，Matsuzawa Y，et al.Pulmonary administration of prostacyclin (PGI2) during partial liquid ventilation in an oleic acid-induced lung injury：inhalation of aerosol or intratracheal instillation? Intensive Care Med，2001，27：243-250.
8. Noble WH，Severinghaus JW.Thermal and conductivity dilution curves for rapid quantitation of pulmonary edema.J Appl Physiol，1972，32：770-775.
9. 钱桂生.在实践中提高对急性肺损伤和急性呼吸窘迫综合征新概念的认识.中国呼吸与危重监护杂志，2002，1：199-201.
10. von der Hardt K，Kandler MA，Fink L，et al.Laser-assisted microdissection and real-time PCR detect anti-inflammatory effect of perfluorocarbon.Am J Physiol Lung Cell Mol Physiol，2003，285：L55-L62.
11. Quintel M，Heine M，Hirschl RB，et al.Effects of partial liquid ventilation on lung injury in a model of acute respiratory failure：a histologic and morphometric analysis.Crit Care Med，1998，26：833-843.
12. 朱光发，张国清，郭海英，等.肺表面活性物质对急性呼吸窘迫综合征肺内炎症反应的影响.中国呼吸与危重监护杂志，2002，1：206-209.
13. 樊毫军，张健鹏，刘又宁.吸入全氟化碳治疗急性肺损伤研究进展.中国呼吸与危重监护杂志，2006，5：63-65.
14. Brower RG，Ware LB，Berthiaume Y，et al.Treatment of ARDS.Chest，2001，120：1347-1367.
15. 彭雪梅，孙婵，王华东.部分液体通气对急性肺损伤小猪肺组织结构及气体交换的影响.中国病理生理杂志，2005，21：129-133.

Chinese Journal of Respiratory and Critical Care Medicine

Effect of aerosolized perfluorocarbon on the tumor necrosis factor-α mRNA expression and lung histopathology in rabbits with acute lung injury

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