The expression and clinical features of macrophage inflammatory protein-1beta in patients with non-small cell lung cancer_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

WANG Hongmei ¹ , NI Songshi ² , LU Minghua ¹

Department of Pulmonary Medicine，Affiliated Hospital of Jiangnan University.Wuxi，Jiangsu，214073，ChinaCorresponding Author：Wang Hong-mei，E-mail：cdy19761108@yahoo.com.cn;

Corresponding author：

Keywords：

Macrophage inflammatory protein-1beta; Non-small cell cancer; Gene.

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Objectives To explore the expression of macrophage inflammatory protein-1beta (MIP-1β) in patients with none-small cell lung cancer (NSCLC) of different pathological types and its association with cancer clinical stages and metastasis of lymph nodes.Methods MIP-1β mRNA from fresh lung tissue of 38 NSCLC patients was amplified by RT-PCR and half-quantified.Immunohistochemical technique was performed to find out the expression of MIP-1β in paraffin-embedded lung tissue from 66 patients with NSCLC.The area and degree of stain were evaluated to determine the positive rate，which was compared between with or without metastasis of lymph nodes，different pathological types and TNM clinical stages.Results MIP-1β protein was found in cytoplasm of malignant cells of squama cell cancer and adenocarcinoma without significant difference between them，while not found in bronchus-alveolus cell cancer.The MIP-1β mRNA expression in squama cell cancer and adenocarcinoma were significant higher than which in bronchus-alveolus cell cancer without significant difference between each other.The positive rates of MIP-1β in lung cancer of Ⅰ，Ⅱ and Ⅲ stages were 74.2%，29.4% and 85.7% respectively，which of Ⅰ and Ⅲ stages cancer were significant higher than Ⅱ stage without significant difference between each other.The positive rates of MIP-1β in lung cancer with or without metastasis of lymph nodes were 45.8% and 76.3% respectively with significant difference between them.Conclusion MIP-1β is expressed in lung cancer cells and relates to the pathological type，TNM stage and the metastasis of lymph nodes.

Citation： WANG Hongmei,NI Songshi,LU Minghua. The expression and clinical features of macrophage inflammatory protein-1beta in patients with non-small cell lung cancer. Chinese Journal of Respiratory and Critical Care Medicine, 2007, 6(2): 119-122. doi: Copy

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1. 曹雪涛.肿瘤免疫.见：陈慰峰，主编.医学免疫学.第4版.北京：人民卫生出版社，2004，224-225.
2. Zlotnik A，Yoshie O.Chemokine：A new classification system and their role in immunity.Immunity，2000，12：121-127.
3. Lu J，Honczarenko M，Sloan SR，et al.Independent expression of the two paralogous CCL4 genes in monocytes and B lymphocytes.Immunogenetics，2004，55：706-711.
4. Moran CJ，Arenberg DA，Huang CC，et al.RANTES expression is a predictor of survival in stage I lung adenocarcinoma.Clin Cancer Res，2002，8：3803-3812.
5. 蒋国梁.肺癌.见：汤钊酋，主编.现代肿瘤学.第2版.上海：复旦大学出版社，2003，862-865.
6. Raychaudhuri B，Abraham S，Bonfield TL，et al.Surfactant blocks lipopolysaccharide signaling by inhibiting both mitogen-activated protein and IkappaB kinases in human alveolar macrophages.Am J Respir Cell Mol Biol，2004，30：228-232.
7. Saito S，Kitayama J，Jin ZX，et al.Beta-chemokine，macrophage inflammatory protein-1beta (MIP-1beta)，is highly expressed in diffuse type human gastric cancers.J Exp Clin Cancer Res，2003，22：453-459.
8. Menten P，Saccani A，Dillen C，et al.Role of the autocrine chemokines MIP-1alpha and MIP-1beta in the metastatic behavior of murine T cell lymphoma.J Leukoc Biol，2002，72：780-789.
9. Miyata T，Yamamoto S，Sakamoto K，et al.Novel immunotherapy for peritoneal dissemination of murine colon cancer with macrophage inflammatory protein-1beta mediated by a tumor- specific vector，HVJ cationic liposomes.Cancer Gene Ther，2001，8：852-860.
10. 罗小玲，张学荣，梁安民，等.MIP-1β基因修饰增强肿瘤相关抗原致敏树突状细胞的抗肿瘤免疫效果.中国肿瘤生物治疗杂志，2004，11：89-91.

Chinese Journal of Respiratory and Critical Care Medicine

The expression and clinical features of macrophage inflammatory protein-1beta in patients with non-small cell lung cancer

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