Expression of the hBD-2 induced by LPS and proinflammatory cytokines in human primary epitheliums_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

LIAO Wei ¹ , QIAN GuiSheng ¹ ,  LEI Han ²

Institute of Respiratory Disease，Xinqiao Hospital，Third Military Medical University.Chongqing，400037，ChinaCorresponding Author：LEI Han，E-mail：leihan@sina.com;

Corresponding author：

LEI Han, Email: leihan@sina.com

Keywords：

Human; Airway epithelium; Human β-defensin-2; Lipopolysaccharide; Proinflammatory cytokines

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Objective To investigate the mRNA and protein expression of human β-defensin-2 (hBD-2) induced by lipopolysaccharide (LPS)，IL-1β and TNF-α in human airway primary epitheliums.Methods The bronchial primary epitheliums from human were stimulated with LPS，IL-1β and TNF-α respectively and then were harvested for hBD-2 expression detection.The mRNA expression of hBD-2 was detected by RT-PCR，and the protein expression by immunocytochemistry and western blot.Results There was a small expression of hBD-2 mRNA in human airway primary epitheliums before stimulation.The hBD-2 mRNA expression was significantly increased after 3 hours of LPS，IL-1β and TNF-α stimulation respectively and the expression increasement was in a dose dependent manner.The hBD-2 protein could be detected in cytoplasm after 4 hours of LPS (0.1 μg/mL)，IL-1β (1 ng/mL) and TNF-α (10 ng/mL) stimulation.Conclusions LPS and proinflammatory cytokines can induce the mRNA and protein expression of hBD-2 in a short time.The expression of hBD-2 may play an initial defense role against bacterial invasion.

Citation： LIAO Wei,QIAN GuiSheng,LEI Han. Expression of the hBD-2 induced by LPS and proinflammatory cytokines in human primary epitheliums. Chinese Journal of Respiratory and Critical Care Medicine, 2007, 6(2): 127-130. doi: Copy

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2. Yang D，Chertov O，Bykovskaia SN，et al.Beta-defensins：linking innate and adaptive immunity through dendritic and T cell CCR6.Science，1999，286：525-528.
3. 黄宁，唐彬，潘小玲，等.人气管上皮细胞气液界面无血清培养.基础医学与临床，2000，20：185-188.
4. Harder J，Bartels E，Christophers，JM，et al.A peptide antibiotic from human skin.Nature，1997，387：861.
5. 张旭，束蓉，罗礼君，等.β-防御素-2多肽在不同类型牙周炎及健康牙龈组织中的表达.上海口腔医学，2006，15：23-27.
6. 李东升，王军，李家文，等.寻常性银屑病患者皮损中抗菌肽LL-37和人β防御素-2 mRNA的表达.临床皮肤科杂志，2005；34：356-358.
7. Hiratsuka T，Mukae H，Iiboshi H，et al.Increased concentrations of human beta-defensins in plasma and bronchoalveolar lavage fluid of patients with diffuse panbronchiolitis.Thorax，2003，58：425-430.
8. Rivas-Santiago B，Schwander SK，Sarabia C，et al.Human beta-defensin 2 is expressed and associated with Mycobacterium tuberculosis during infection of human alveolar epithelial cells.Infect Immun，2005，73：4505-4511.
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Chinese Journal of Respiratory and Critical Care Medicine

Expression of the hBD-2 induced by LPS and proinflammatory cytokines in human primary epitheliums

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