Objective To investigate the gene expression of beta-defensin-4 (mBD-4) and mBD-6 in acute lung injury (ALI) mouse.Methods Sixty adult mice were randomly divided into a control group and a ALI group.ALI was induced by intraperitoneal injection of lipopolysaccharide (LPS) in the ALI group.The control group was treated with same dose of normal saline.The lung tissues were harvested at different time point after stimulation.The expression of mBD-4 and mBD-6 mRNA was measured by real-time quantitative reverse transcription polymerase chain reaction.DNA sequencing was used to confirm the specificity of mBD-4 and mBD-6 cDNA fragment.Results There were no obvious mBD-4 and mBD-6 mRNA expression in mouse lung in the control group at all time points and ALI 6 h group.In the ALI group a marked increasing expression was found on 12 h，1 d and 3 d after LPS stimulation.The mBD-4 mRNA expression was significant higher in the ALI groups of 1 d and 3 d points than that of ALI 12 h group with no obvious difference between each other.There were no significant differences of mBD-6 mRNA expression between ALI groups of 12 h，1 d and 3 d points Conclusion mBD-4 and mBD-6 mRNA is not constitutive expressed in mouse lung and show a up-regulative expression pattern after ALI.
Citation：
TIAN Cuihuan,YU Senyang. Gene expression of β-defensin-4 and β-defensin-6 in lung tissue of acute lung injury mouse. Chinese Journal of Respiratory and Critical Care Medicine, 2007, 6(2): 131-134. doi:
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- 1. Harder J，Bartels J，Chritophers E，et al.A peptide antibiotic from human skin.Nature，1997，387：861.
- 2. Yang D，Chertov O，Bykovskaia SN，et al.Beta-defensins：linking innate and adaptive immunity through dendritic and T cell CCR6.Science，1999，286：525-528.
- 3. Bals R，Wang X，Wu Z，et al.Human β-defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung.J Clin Invest，1998，102：874-880.
- 4. Hiratsuka T，Nakazato M，Date Y，et al.Identification of human beta-defensin-2 in respiratory tract and plasma and its increase in bacterial pneumonia.Biochem Biophys Res Commun，1998，249：943-947.
- 5. Jia HP，Wowk SA，Schutte BC，et al.A noval murine beta-defensin expressed in tongue，esophagus，and trachea.J.Biol.Chem，2000，275：33314-33320.
- 6. Yamaguchi Y，Fukuhara S，Nagase T，et al.A novel mouse beta-defensin，mBD-6，predominantly expressed in skeletal muscle.J Biol Chem，2001，276：31510-31514.
- 7. Jia HP，Mills JN，Barahmand-Pour F，et al.Molecular cloning and characterization of rat genes encoding homologues of human beta-defensins.Infec Immun，1999，67：4827-4833.
- 8. Liu L，Wang L，Jia HP，et al.Structure and mapping of the human beta- defensin hBD-2 gene and its expression at sites of inflammation.Gene，1998，222：237-244.
- 9. Diamond G，Kaiser V，Rhodes J，et al.Transcription regulation of beta-defensin gene expression in tracheal epithelial cells.Infect Immun，2000，68：113-119.
