Objective To investigate the antibiotic resistance and their genetic homology of stenotrophomonas maltophilia isolated from January 2005 to February 2006 at intensive care unit（ICU） of 6 hospitals in Bejing area.Methods The minimal inhibitory concentration（MIC） of 12 antibiotics against 82 strenotrophomonas maltophilia was determined by broth dilution method.PFGE was used to analyze the homology of 82 stenotrophomonas maltophilia.Results The drug sensitivity tests in vitro showed these strains were resistance to commonly-used antibiotics.Antibiotics with sensitive rate over 50% included Doxycycline, gatifloxacin,cefoperazone-sulbactam,levofloxacin,Compound sulfamethoxazole,Ceftazidime and ticarcillin- clavulanate. 7-18 DNA bands of different size were present in the gel and different homology was shown among the 82 strains.Four couples with homology over 85% were isolated from the same ICU.Three strain were same clones in PLA General Hospitals first hospital.2 couples from the different wards had homology of 80.6% and 79.6% of，respictively.Others strains had either poor or no homology.Conclusions No clonal outbreak is certified at ICU of 6 hospitals in Beijing area. There are only vertical dissemination of single clone in 6 ICU wards.PFGE is an effective approach for drug resistance test and epidemic analysis.
Citation：
DENG Xiaowei,LIU Changting,ZHENG Jingchen,LIANG Liwu,LI Yan,LI Hui,WANG Haiyan.. Drug resistance and its genetic homology of stenotrophomonas maltophilia at department of intensive care unit of 6 hospitals in Beijing. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(1): 15-19. doi:
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孙荣青，冯敏，徐珉，等.重症监护病房革兰阴性杆菌的感染分布与耐药性监测.中国呼吸与危重监护杂志，2004，3：298-299.
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卓超，钱元恕，李崇智，等.产金属β-内酰胺酶嗜麦芽寡养单胞菌的研究.中国抗生素杂志，2003，28：753-756.
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孔晖，赵珊.嗜麦芽窄食单胞菌呼吸机相关性肺炎12例临床分析.中国呼吸与危重监护杂志，2003，9：309.
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黎毅敏，黄红川，刘晓青，等.重症监护病房铜绿假单胞菌医院获得性感染暴发的流行病学调查.中国呼吸与危重监护杂志，2006，11：413-417.
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Lavigne JP，Bouziges N，Chanal C，et al.Molecular epidemiology of Enterobacteriaceae isolates producing extended-spectrum beta-lactamases in a French hospital.J Clin Microbiol，2004，42：3805-3808.
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- 1. 孙荣青，冯敏，徐珉，等.重症监护病房革兰阴性杆菌的感染分布与耐药性监测.中国呼吸与危重监护杂志，2004，3：298-299.
- 2. 岳文香，黄绍光，聂森，等.重症监护室嗜麦芽窄食单胞菌的分子流行病学研究.中华结核和呼吸杂志，2001，24：651-654.
- 3. 卓超，李小青.重症监护病病房下呼吸道感染或定植嗜麦芽窄食单胞菌患者48例分析.中华烧伤杂志，2006，22：88-91.
- 4. Barbier-Frebour N，Boutiba-Boubake I，Nouvello M，et al.Molecular investigation of Stenotrophomonas maltophilia isolates exhibiting rapid emergence of ticarcillin-clavulanate resistance.J Hosp Infect，2000，45：35-41.
- 5. Chang LL，Chen HF，Chang CY，et al.Contribution of integrons，and SmeABC and SmeDEF efflux pumps to multidrug resistance in clinical isolates of Stenotrophomonas maltophilia.J Antimicrob Chemother，2004，53：518-521.
- 6. 卓超，钱元恕，李崇智，等.产金属β-内酰胺酶嗜麦芽寡养单胞菌的研究.中国抗生素杂志，2003，28：753-756.
- 7. 孔晖，赵珊.嗜麦芽窄食单胞菌呼吸机相关性肺炎12例临床分析.中国呼吸与危重监护杂志，2003，9：309.
- 8. 黎毅敏，黄红川，刘晓青，等.重症监护病房铜绿假单胞菌医院获得性感染暴发的流行病学调查.中国呼吸与危重监护杂志，2006，11：413-417.
- 9. Lavigne JP，Bouziges N，Chanal C，et al.Molecular epidemiology of Enterobacteriaceae isolates producing extended-spectrum beta-lactamases in a French hospital.J Clin Microbiol，2004，42：3805-3808.
