Objective To observe the effects of hydrogen peroxide on the expression of transforming growth factorβ1(TGF-β1) and Smad3 protein in A549 cells. Methods A549 cells were cultured with different concentrations of hydrogen peroxide. MTT assay was used to determine the cell growth and survival rates. The level of TGF-β1 and p-Smad3 protein were detected by western blotting. Results It was observed that hydrogen peroxide significantly inhibit proliferation of A549 cells. When the concentration of hydrogen peroxide was 1.0 mmol/L, the inhibition ratio reaches 46.34%, and the level of TGF-β1 and p-Smad3 protein were increased in a time-dependence manner and reached a peak after 24 h, then decreased a little but also remained at high level. Conclusions In the early oxidative damage, A549 cells express high level of TGF-β1 and p-Smad3 protein. It may be relevant to tissue repair and remodeling after lung injury.
Citation：
YANG Dongmei,DING Yu,YING Binwu,JIANG Fei,XU Dan,MEILANG QUCUO,FAN Hong. Effects of hydrogen peroxide on expression of transforming growth factor-β1 and Smad3 in A549 cells. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(1): 43-46. doi:
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- 1. Matthay MA, Geiser T, Matalon S,et al.Oxidant-mediated lung injury in the acute respiratory distress syndrome. Crit Care Med, 1999,27:2028-2030.
- 2. Chabot F, Mitchell JA, Gutteridge JM, et al. Reactive oxygen species in acute lung injury. Eur Respir J, 1998,11:745-757.
- 3. Waxman AB, Mahboubi K, Knickelbein RG,et al. Interleukin-11 and interleukin-6 protect cultured human endothelial cells from H2O2-induced cell death. Am J Respir Cell Mol Biol, 2003,29:513-522.
- 4. Bellingan GJ.The pulmonary physician in critical care 6: The pathogenesis of ALI/ARDS.Thorax,2002,57:540-546.
- 5. 陈谦.二喹啉甲酸（BCA）检测法.见：汪家政，范明，主编.蛋白质技术手册．北京：科学出版社，2001，51-54．.
- 6. Fehrenbach H. Alveolar epithelial type II cell: defender of the alveolus revisited. Respir Res,2001,2:33-46.
- 7. 张静，吴瑜.N-乙酰-L-半胱氨酸对H2O2引起的血管内皮细胞损伤的拮抗效应.中国呼吸与危重监护杂志，2003，2：91-94.
- 8. Derynck R, Zhang YE. Smad-dependent and Smad-independent pathways in TGF-beta family signalling. Nature,2003,425:577-584.
- 9. Ebner R, Chen RH, Lawler S, et al . Determination of type I receptor specificity by the type II receptors for TGF-beta or activin.Science,1993,262:900-902.
- 10. Verrecchia F, Chu ML, Mauviel A.Identification of novel TGF-beta /Smad gene targets in dermal fibroblasts using a combined cDNA microarray/promoter transactivation approach.J Biol Chem,2001,276:17058-17062.
- 11. Muthukrishnan L, Warder E, McNeil PL.Basic fibroblast growth factor is efficiently released from a cytolsolic storage site through plasma membrane disruptions of endothelial cells. J Cell Physiol,1991,148:1-16.
