• Department of Digestion,Tianhao Affiliated Hospital of Lanzhou University.Lanzhou,Gansu,730060,China. Corresponding author:FU Ming-sheng,E-mail:msf768300@163.com;
FU Mingsheng , Email: msf768300@163.com
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Objective   To investigate the effects of inhaled prostaglandin E1 (PGE1)on Th1/Th2 polarity in rat model of lipopolysaccharide(LPS) induced acute lung injury(ALI).Methods  Healthy adult male Wistar rats [weight (200±20)g] were randomly divided into normal control(NS) group,LPS group and PGE1 group.The model of ALI were established by injecting LPS of 5 mg/kg into caudal vein.The rats in PGE1 group inhaled aerosolized PGE1(2 μg/mL)for 30 minutes after LPS injection,then repeat the procedure 12 hours later. 1 h,6 h,12 h and 24 h after last PGE1 inhalation,enzyme linked immunosorbant assay (ELISA) was empolyed to measure the level of interferon-γ(IFN-γ)and interleukin-4(IL-4)in the serum and bronchoalveolar lavage fluid(BALF)and the ratio of IFN-γ/IL-4(Th1/Th2)was calculated.Pathological examination was made under light microscope.Results   Pathological examination of lung tissue demonstrated success ALI model.Compared to NS group,the ratio of IFN-γ/IL-4(Th1/Th2)both in serum and BALF in LPS group elevated significantly(P lt;0.01). PGE1 administration significantly decreased the ratio IFN-γ/IL-4 in serum after 6h(P lt;0.01)and in BALF at all time points(P lt;0.01).Conclusion   The imbalance of was found in the LPS induced ALI,inhaled PGE1 aerosol inhalation could restore Th1/Th2 cytokine balance in the rats model induced by LPS.

Citation: FU Mingsheng ,XIAO Xiaohui,WAN Yixin.. The effects of inhaled prostaglandin E1 on Th1/Th2 lipopolysaccharideinduced acute lung injury in rats. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(1): 47-51. doi: Copy

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