Objective To examine the effects of Pseudomonas aeruginosa(PA)quorum-sensing systems on airway mucus hypersecretion．Methods Sixty Sprague-Dawley rats were intubated with a silicone tube pre-coated with PAO1(wild-type PA strain)，PAO1-JP2(quorum-sensing-mutant strain)or saline in the bronchus．After 28 days，the mRNA and protein expression of MUC5AC in the rats’bronchial epithelia were detected by RT-PCR，alcian blue／periodic acid—Schif(AB／PAS)staining and enzyme linked immunosorbent assay(ELISA)．Results In the PAO1 group，bronchiolar epithelium goblet cells by AB／PAS staining was significantly more than those in the PAO1-JP2 and control groups(both P lt;0．05)．The expression level of MUC5AC mRNA in the PAO1 group was significantly higher than those in the PAO1-JP2 and control groups(both P lt;0．05)．The ELISA showed that the concentration of MUC5AC protein in bronchoalveolar lavage fluid(BALF)in the PAO1 group was much higher than that in the PAO1-JP2 group(P lt;0．05)．Conclusion PA quorum-sensing system plays an important role in airway mucus hypersecretion
Citation：
ZHANG Ping,YANG Qimei,SHEN Ce. Efect of Pseudomonas aeruginosa quorum-sensing systems on airway mucus hypersecretion. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(2): 93-96. doi:
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de Kievit TR，Iglewski BH．Bacterial quorum sensing in pathogenic relationships．Infect Immun，2000，68：4839-4849．.
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Imamura Y，Yanagihara K，Mizuta Y，et al．Azithromycin inhibits MUC5AC production induced by the Pseudomonas aeruginosa autoinducer N一(3-Oxododecanoy1)homoserine lactone in NCI．H292 Cells．Antimicrob Agents Chemother．2004，48：3457-3461．.
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Mill MB，Bassler BL．Quorum sensing in bacteria．Annu Rev Microbiol，2001，55：165-199．.
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Schaber JA，Carry NL，McDonald NA，et al．Analysis of quorum sensing-·deficient clinical isolates of Pseudomonas aeruginosa．J Med Microbiol，2004，53：841．853．.
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He SH，Zheng J，Duan MK．Induction of mucin secretion from human bronchial tissue and epithelial cells by rhinovirus and lipopolysaccharide．Acta Pharmacol Sin，2004．25：1176．1181．.
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Li JD，Feng W ，Gallup M，et al．Activation of NF-kappaB via a Src-dependent Ras-·MAPK··pp90rsk pathway is required for Pseudomonas aeruginosa induced mucin overproduction in epithelial cells．Proc Natl Acad Sci USA，1998，95：5718-5723．.
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Kohri K，Ueki IF，Shim JJ，et al．Pseudomonas aeruginosa induces MUC5AC production via epidermal growth factor receptor．Eur Respir J，2002，20：1263-1270．.
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| 8. |
Nouwens AS，Beatson SA，Whitchurch CB，et al．Proteome analysis of extracellular proteins regulated by the las and rhl quorum sensing systems in Pseudomonas aeruginosa PAO1．Microbiology，2003，149：13 1 1·1322.
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- 1. de Kievit TR，Iglewski BH．Bacterial quorum sensing in pathogenic relationships．Infect Immun，2000，68：4839-4849．.
- 2. Imamura Y，Yanagihara K，Mizuta Y，et al．Azithromycin inhibits MUC5AC production induced by the Pseudomonas aeruginosa autoinducer N一(3-Oxododecanoy1)homoserine lactone in NCI．H292 Cells．Antimicrob Agents Chemother．2004，48：3457-3461．.
- 3. Mill MB，Bassler BL．Quorum sensing in bacteria．Annu Rev Microbiol，2001，55：165-199．.
- 4. Schaber JA，Carry NL，McDonald NA，et al．Analysis of quorum sensing-·deficient clinical isolates of Pseudomonas aeruginosa．J Med Microbiol，2004，53：841．853．.
- 5. He SH，Zheng J，Duan MK．Induction of mucin secretion from human bronchial tissue and epithelial cells by rhinovirus and lipopolysaccharide．Acta Pharmacol Sin，2004．25：1176．1181．.
- 6. Li JD，Feng W ，Gallup M，et al．Activation of NF-kappaB via a Src-dependent Ras-·MAPK··pp90rsk pathway is required for Pseudomonas aeruginosa induced mucin overproduction in epithelial cells．Proc Natl Acad Sci USA，1998，95：5718-5723．.
- 7. Kohri K，Ueki IF，Shim JJ，et al．Pseudomonas aeruginosa induces MUC5AC production via epidermal growth factor receptor．Eur Respir J，2002，20：1263-1270．.
- 8. Nouwens AS，Beatson SA，Whitchurch CB，et al．Proteome analysis of extracellular proteins regulated by the las and rhl quorum sensing systems in Pseudomonas aeruginosa PAO1．Microbiology，2003，149：13 1 1·1322.
