Objective To investigate the expression of aquaporin-1（AQP-1） on pleura in rats with carrageenan-induced pleural effusion and explore the role of AQP-1 in effusion formation.Methods Fifty-six healthy Wistar rats were randomly divided into a normal control group and 6 pleuritis groups（6，12，24，36，48 and 72 h groups respectively）.The rat model of inflammatory pleurisy was induced by injecting l-Carrageenan into the pleural cavity.The expression of AQP-1 on pleura was detected with immunohistochemistry.The mRNA and protein expression of AQP-1 on visceral pleura and parietal pleura were measured by RT-PCR and Western blot assay respectively.The volume of pleural effusions were measured.Results The volume of pleural effusion was 2.10±0.22，4.10±0.15，4.40±0.36，3.20±0.27，2.60±0.18，0.12±0.02 mL in the 6，12，24，36，48 and 72 h pleuritis groups respectively.AQP-1 were mainly expressed on visceral and parietal pleural mesothelial cells and capillary endothelial cells，and significantly increased in all pleuritic rats The mRNA and protein expression of AQP-1 on parietal pleura increased after 6 h and reached peak level at 24 h in pleuritic groups.The mRNA and protein expression of AQP-1 on visceral pleura increased after 12 h and reached peak level at 24 h in pleuritic groups.The expression of AQP-1 on parietal pleura at 12 h and 24 h in pleuritic groups was correlated positively with the volume of pleural effusion（r=0.857，r=0.846，all P lt;0.01）.The expression of AQP-1 on visceral pleura at 24 h in pleuritic groups was positively correlated with the volume of pleural effusion（r=0.725，P lt;0.05）.Conclusion The expression of AQP-1 on pleura were increased in rats with e carrageenan-induced pleural effusion.AQP-1 may play a role in pleural fluid transportation in pleural effusion.
Citation：
ZHANG Wei,XIE Canmao. Expression of aquaporin-1 in rat model of carrageenan-induced pleural effusion. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(3): 190-194. doi:
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Light RW，LeeYCG..Textbook of Pleural Diseases.Firsted.Oxford University Press Inc，London，2003.xiii.
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Manley GT，Fujimura M，Ma T，et al.Aquaporin-4 deletion in mice reduces brain edema after acute water intoxication and ischemic stroke.Nat Med，2000，6：159-163.
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孙明瑜，谢鸣，张娜，等.小柴胡汤及其药群配伍对角叉菜胶诱导大鼠胸膜炎模型细胞因子的影响.中国中西医结合杂志，2004，24：628-631.
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Corsini E，Di Paola R，Viviani B，Genovese T，Mazzon E，Lucchi L，Marinovich M，Galli CL，Cuzzocrea S.Increased carrageenan-induced acute lung inflammation in old rats.Immunology.2005 Jun；115：253-61.
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Marzocco S，Di Paola R，Serraino I，et al.Effect of methylguanidine in carrageenan-induced acute inflammation in the rats.Eur J Pharmacol，2004，484：341-350.
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Sakaguchi Y，Shirahase H，Kunishiro K，et al.Effect of combination of nitric oxide synthase and cyclooxygenase inhibitors on carrageenan-induced pleurisy in rats.Life Sci，2006，79：442-447.
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Di Paola R，Mazzon E，Muia C，et al.Green tea polyphenol extract attenuates lung injury in experimental model of carrageenan-induced pleurisy in mice.Respir Res，2005，6：66.
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Agre P.The aquaporin water channels.Proc Am Thorac Soc，2006，3：5-13.
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King LS，Nielsen S，Agre P.Aquaporins and the respiratory system：advice for a lung investigator.J Clin Invest，2000，105：15-16.
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Bai C，Fukuda N，Song Y，et al.Lung fluid transport in aquaporin-1 and aquaporin-4 knockout mice.J Clin Invest，1999，103：555-561.
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Antony VB.Immunological mechanisms in pleural disease.Eur Respir J，2003，21：539-44.
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- 1. Light RW，LeeYCG..Textbook of Pleural Diseases.Firsted.Oxford University Press Inc，London，2003.xiii.
- 2. Manley GT，Fujimura M，Ma T，et al.Aquaporin-4 deletion in mice reduces brain edema after acute water intoxication and ischemic stroke.Nat Med，2000，6：159-163.
- 3. Cuzzocrea S，Mazzon E，Calabro G，et al.Inducible nitric oxide synthase-knockout mice exhibit resistance to pleurisy and lung injury caused by carrageenan.Am J Respir Crit Care Med，2000，162：1859-1866.
- 4. 孙明瑜，谢鸣，张娜，等.小柴胡汤及其药群配伍对角叉菜胶诱导大鼠胸膜炎模型细胞因子的影响.中国中西医结合杂志，2004，24：628-631.
- 5. Corsini E，Di Paola R，Viviani B，Genovese T，Mazzon E，Lucchi L，Marinovich M，Galli CL，Cuzzocrea S.Increased carrageenan-induced acute lung inflammation in old rats.Immunology.2005 Jun；115：253-61.
- 6. Marzocco S，Di Paola R，Serraino I，et al.Effect of methylguanidine in carrageenan-induced acute inflammation in the rats.Eur J Pharmacol，2004，484：341-350.
- 7. Sakaguchi Y，Shirahase H，Kunishiro K，et al.Effect of combination of nitric oxide synthase and cyclooxygenase inhibitors on carrageenan-induced pleurisy in rats.Life Sci，2006，79：442-447.
- 8. Di Paola R，Mazzon E，Muia C，et al.Green tea polyphenol extract attenuates lung injury in experimental model of carrageenan-induced pleurisy in mice.Respir Res，2005，6：66.
- 9. Agre P.The aquaporin water channels.Proc Am Thorac Soc，2006，3：5-13.
- 10. King LS，Nielsen S，Agre P.Aquaporins and the respiratory system：advice for a lung investigator.J Clin Invest，2000，105：15-16.
- 11. Bai C，Fukuda N，Song Y，et al.Lung fluid transport in aquaporin-1 and aquaporin-4 knockout mice.J Clin Invest，1999，103：555-561.
- 12. Antony VB.Immunological mechanisms in pleural disease.Eur Respir J，2003，21：539-44.
