Objective To study the effect of nontypeable Hemophilus influenzae(NTHi) strain ATCC49247 on proinflammatory cytokines expression of human A549 lung epithelial cell line. Methods Confluent A549 cells were co-incubated with NTHi, NTHi+Erythromycin(10 mg/L), NTHi+Gentamicin(100 mg/L), and NTHi+Dexamethasone(100 μmol/L),and nuclear factor kappa B(NF-κB) inhibitor primed cells were co-incubated with NTHi for 24 h. Then levels of interleukin-8（IL-8） and tumor necrosis factor-α(TNF-α) in the supernatant was assayed by enzyme-linked immunosorbent assay(ELISA) and the expression of intercellular adhesion molecule-1(ICAM-1) in cells was detected by immunohistochemistry staining. Results A549 cells were transformed and died after co-intubated with NTHi for 24 h. NTHi induced A549 cells to release significantly greater amounts of IL-8, which was inhibited by NF-κB inhibitor pyrrolidine dithiocarbamate（PDTC）. Incubating of A549 cells with NTHi significantly induced release of IL-8 and the expression of ICAM-1, which was blocked by erythromycin and dexamethasone and not by gentamicin. TNF-α was not detected in all circumstances. Conclusions NTHi can increase significantly the release and expression of proinflammatory cytokines through NF-κB pathway. Antibacterial drug erythromycin also has anti-inflammatory effect.
Citation：
XIU Qingyu,QIAN Jianmei,WANG Guifang,Huang Hai,FANG Zheng. The effects of nontypeable Hemophilus influenzae on proinflammatory cytokine expression of human lung epithelial cell line. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(3): 210-213. doi:
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Copyright © the editorial department of Chinese Journal of Respiratory and Critical Care Medicine of West China Medical Publisher. All rights reserved
| 1. |
1
方正，修清玉，罗文侗. 慢性阻塞性肺疾病中的流感嗜血杆菌感染. 第二军医大学学报 1999, 20:624-626.
|
| 2. |
4
钱建美，修清玉，王桂芳,等.不可分型流感嗜血杆菌对气管黏膜上皮细胞的作用.第二军医大学学报,2004，25：769-772.
|
| 3. |
Sethi S, Evans N, Grant BJ, et al. New strains of bacteria and exacerbations of chronic obstructive pulmonary disease. N Engl J Med, 2002, 347:465-471.
|
| 4. |
Murphy TF, Brauer AL, Schiffmacher AT,et al.Persistent colonization by Haemophilus influenzae in chronic obstructive pulmonary disease. Am J Respir Crit Care Med, 2004,170:266–272.
|
| 5. |
Khair OA, Devalia JL, Abdelaziz MM, et al. Effect of Haemophilus influenzae endotoxin on the synthesis of IL-6,IL-8,TNF-α and expression of ICAM-1 in cultured human bronchial epithelial cells. Eur Respir J,1994,7:2109-2116.
|
| 6. |
Marcet B, Libert F, Boeynaems JM, et al. Extracellular nucleotides induce COX-2 up-regulation and prostaglandin E (2) production in human A549 alveolar type II epithelial cells. Eur J Pharmacol, 2007, 566: 167-171.
|
| 7. |
Carterson AJ, Höner zu Bentrup K, Ott CM, et al .A549 lung epithelial cells grown as three-dimensional aggregates: alternative tissue culture model for Pseudomonas aeruginosa pathogenesis. Infect. Immun, 2005, 73:1129-1140.
|
| 8. |
Clemans DL, Bauer RJ, Hanson JA, et al. Induction of proinflammatory cytokines from human respiratory epithelial cells after stimulation by nontypeable Haemophilus influenzae. Infect Immun, 2000, 68:4430-4440.
|
| 9. |
Frick AG, Joseph TD, Pang LY, et al. Haemophilus influenzae stimulates ICAM-1 expression on respiratory epithelial cells. J Immunol, 2000, 164:4185-4196.
|
- 1. 1
方正，修清玉，罗文侗. 慢性阻塞性肺疾病中的流感嗜血杆菌感染. 第二军医大学学报 1999, 20:624-626.
- 2. 4
钱建美，修清玉，王桂芳,等.不可分型流感嗜血杆菌对气管黏膜上皮细胞的作用.第二军医大学学报,2004，25：769-772.
- 3. Sethi S, Evans N, Grant BJ, et al. New strains of bacteria and exacerbations of chronic obstructive pulmonary disease. N Engl J Med, 2002, 347:465-471.
- 4. Murphy TF, Brauer AL, Schiffmacher AT,et al.Persistent colonization by Haemophilus influenzae in chronic obstructive pulmonary disease. Am J Respir Crit Care Med, 2004,170:266–272.
- 5. Khair OA, Devalia JL, Abdelaziz MM, et al. Effect of Haemophilus influenzae endotoxin on the synthesis of IL-6,IL-8,TNF-α and expression of ICAM-1 in cultured human bronchial epithelial cells. Eur Respir J,1994,7:2109-2116.
- 6. Marcet B, Libert F, Boeynaems JM, et al. Extracellular nucleotides induce COX-2 up-regulation and prostaglandin E (2) production in human A549 alveolar type II epithelial cells. Eur J Pharmacol, 2007, 566: 167-171.
- 7. Carterson AJ, Höner zu Bentrup K, Ott CM, et al .A549 lung epithelial cells grown as three-dimensional aggregates: alternative tissue culture model for Pseudomonas aeruginosa pathogenesis. Infect. Immun, 2005, 73:1129-1140.
- 8. Clemans DL, Bauer RJ, Hanson JA, et al. Induction of proinflammatory cytokines from human respiratory epithelial cells after stimulation by nontypeable Haemophilus influenzae. Infect Immun, 2000, 68:4430-4440.
- 9. Frick AG, Joseph TD, Pang LY, et al. Haemophilus influenzae stimulates ICAM-1 expression on respiratory epithelial cells. J Immunol, 2000, 164:4185-4196.
