Objective To examine the levels of nitrite /nitrate( NO2 /NO3 ) , IL-4 and IFN-γin exhaled breath condensates( EBCs) in patients with asthma, and to investigate the effect of corticosteroid treatment. Methods Forty patients diagnosed as mild to moderate asthma were recruited. Among them, twenty were newly diagnosed and steroid naive( non-treatment group) . The other 20 patients had been treated with corticosteroid treatment( treatment group) . Twenty healthy volunteers were enrolled as normal control.EBC samples were taken for measurement of NO2 /NO3, IL-4 and IFN-γlevels. Serum IL-4 and IFN-γ concentrations were also measured. Results NO2 /NO3 level in EBC of the non-treatment group was significantly higher than that of the normal controls and the treatment group[ ( 48. 55 ±27. 37) μmol / L vs
( 24. 51 ±18. 22) μmol /L, ( 36. 06 ±25. 13) μmol /L, respectively, both P lt; 0. 05] , and no significant difference was found between the treatment group and the normal controls( P gt;0. 05) . The IL-4 and IFN-γ levels in both EBC and serum had no significant difference between the three groups ( all P gt; 0. 05) . Conclusion NO2 /NO3 in EBCs increases in asthmatic patients, and decreases after corticosteroid treatment. Whether IL-4 and IFN-γlevels can be used for reflecting airway inflammation need further study.
Citation：
CHEN Yanwen,HE Quanying,SHANG Ying,GAI Jun.. Clinical Significance of Nitrite/Nitrate, Interleukin-4 and Interferon-gamma in Exhaled Breath Condensate of Asthmatic Patients. Chinese Journal of Respiratory and Critical Care Medicine, 2009, 09(2): 131-134. doi:
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Shahid SK, Kharitonov SV, Wilson NM, et al. Increases interleukin-4 and decreased interferon-gamma in exhaled breath condensate of children with asthma. Am J Respir Crit Care Med, 2002, 165 : 1290 -1293.
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Leung TF, Wong GW, Ko FW, et al. Analysis of growth factors and inflammatory cytokines in exhaled breath condensate from asthmatic children. Int Arch Allergy Immunol, 2005, 137: 66-72.
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Carpagnano GE, Foschino Barbaro MP, Resta O, et al. Exhaled markers in the monitoring of airways inflammation and its response to steroid’s treatment in mild persistent asthma. Eur J Pharmacol,2005, 519: 175-181.
|
- 1. Jeffery PK, Laitinen A, Venge P. Biopsy markers of airway inflammation and remodeling. Respir Med, 2000, 94( suppl F) : S9-S15.
- 2. Kharitonov SA, Barnes PJ. Biomarkers of some pulmonary diseases in exhaled breath. Biomarkers, 2002, 7: 1 -32 .
- 3. American Thoracic Society. Standardization of spirometry 1994. Am J Respir Crit Care Med, 1995, 152: 1107-1136.
- 4. Hanazawa T, Kharitonov SA, Barnes PJ. Increased nitrotyrosine in exhaled breath condensate of patients with asthma. Am J Respir Crit Care Med, 2000, 162: 1273-1276.
- 5. Corradi M, Pesci A, Casana R, et al. Nitrate in exhaled breath condensate of patients with different airway diseases. Nitric Oxide,2003, 8: 26-30.
- 6. Formanek W, Inci D, Lauener RP, et al. Elevated nitrite in breath condensate of children with respiratory disease. Eur Respir J, 2002 ,19: 487-491.
- 7. Ganas K, Loukides S, Papatheodorou G, et al. Total nitrite/ nitrate in expired breath condensate of patients with asthma. Respir Med, 2001, 95: 649-654.
- 8. Shahid SK, Kharitonov SV, Wilson NM, et al. Increases interleukin-4 and decreased interferon-gamma in exhaled breath condensate of children with asthma. Am J Respir Crit Care Med, 2002, 165 : 1290 -1293.
- 9. Leung TF, Wong GW, Ko FW, et al. Analysis of growth factors and inflammatory cytokines in exhaled breath condensate from asthmatic children. Int Arch Allergy Immunol, 2005, 137: 66-72.
- 10. Carpagnano GE, Foschino Barbaro MP, Resta O, et al. Exhaled markers in the monitoring of airways inflammation and its response to steroid’s treatment in mild persistent asthma. Eur J Pharmacol,2005, 519: 175-181.
